Protection of swimming-induced oxidative stress in some vital organs by the treatment of composite extract of <i>Withania somnifera, Ocimum sanctum</i> and <i>Zingiber officinalis</i> in male rat

Abstract

Composite methanolic extract of roots of Withania somnifera, leaves of Ocimum sanctum, and rhizomes of Zingiber officinalis was administered by gavage at the dose of 40 mg 100 g −1 body weight day −1 to rat orally for 15 days prior to experimentation followed by co-administration of above extract at the same dose for 28 days of swimming to find out the remedial effect of this extract on exhaustive physical exercise-induced oxidative damage. Swimming resulted significant diminution (p<0.05) in the activities of catalase and superoxide dismutase along with elevation (p<0.05) in the levels of thiobarbituric acid reactive substances and conjugated dienes in cardiac, skeletal, hepatic tissues, cerebrum and cerebellum in respect to control. The levels of all these parameters were resettled significantly (p<0.05) towards the extract pretreated cum co-treated swimming group. The antioxidative potency of this composite extract was compared with standard non-enzymatic antioxidant (vitamin-E) in forced swimming state. The above extract has no general toxic effect as reflected here from the study of transaminase activities in liver and kidney. Results lead to conclude that the composite extract of above three plant parts has a therapeutic protective effect on forced swimming-induced oxidative stress in vital organs.

Effects of Gynostemma pentaphyllum (Thunb.) Makino polysaccharides supplementation on exercise tolerance and oxidative stress induced by exhaustive exercise in rats

“…Malondialdehyde (MDA) has been the most widely used parameter for evaluating oxidative damage to lipids, although, it is known that oxidative damage to amino acids, proteins and DNA also causes release of MDA. Previous studies had indicated that exhaustive exercise causes an increase in MDA and the MDA increasing due to excess oxygen radical reacting polyunsaturated acid in the muscle (Misra et al, 2009;Sun et al, 2010). The present study demonstrated that the GPMP supplementation can promote increases in the activities of these antioxidant enzymes (SOD, GPH-Px and CAT) and reduce lipid peroxidation.…”

“…It is known that antioxidant enzymes exhibit synergistic interactions by protecting each other from specific free radical attacks (Perse et al, 2009). The significant decrease in the activities of SOD, GPH-Px and CAT in the muscle tissue after forced swimming may be an indication of exercise-induced oxidative threat (Misra et al, 2009). Malondialdehyde (MDA) has been the most widely used parameter for evaluating oxidative damage to lipids, although, it is known that oxidative damage to amino acids, proteins and DNA also causes release of MDA.…”

Polysaccharides from <i>Portulaca oleracea</i> L Improve Exercise Endurance and Decrease Oxidative Stress in Forced Swimming Mice

“…After the final treatment with POP, the mice were taken out for the forced swimming exercise. The procedure used was described previously with some modifications [12]. Briefly, the mice were dropped individually into an acrylic plastic pool (50 cm × 50 cm × 40 cm) filled with fresh water maintained at 25 ± 2 °C, approximately 30 cm deep so that mice could not support themselves by touching the bottom with their tails.…”

Protective effects of Radix Pseudostellariae polysaccharides against exercise-induced oxidative stress in male rats

“…Exhaustive swimming was selected as a model of physical exercise since muscle trauma caused by other types of physical exercise, including prolonged running on a treadmill, exercise stimulated by electric shock and plyometric contractions, may be avoided (20,21). As shown in Fig.…”