Increase in cytosolic and mitochondrial protein synthesis in rat hepatocytes irradiated in vitro by HeNe laser

Vacca, Rosa Anna; Marra, Ersilia; Passarella, Salvatore; Petragallo, Vito A.; Greco, Margherita

doi:10.1016/1011-1344(95)07280-2

Cited by 30 publications

(21 citation statements)

References 14 publications

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“…It has been argued that the biological response of cells to monochromatic light involves activation of a photoacceptor molecule followed by a cascade of biochemical reactions in the time scale of minutes and hours after irradiation [24]. For example, an increase in intracellular calcium and activation of the ATP-dependent K + ion channel was observed within minutes after irradiation [25,26], while RNA and protein synthesis peaks at 1 h after irradiation [3,4]. Therefore, it appears that the protective effect of He-Ne laser involves the induction of biochemical reactions which needs further investigations.…”

Section: Discussionmentioning

confidence: 99%

“…He-Ne laser irradiation (632.8 nm) of cells has been reported to result in a variety of effects on cell structure and function, such as remodelling of the cytoskeletal network [1], formation of giant mitochondria [2], enhanced protein and DNA synthesis [3,4,5] and ATP (adenosine triphosphate) generation [6]. Pre-exposure to He-Ne laser irradiation has been observed to modulate the damaging effects of ionising radiations and to lead to a decrease in x-ray sensitivity of E. coli K-12 [7] as well as in the cytotoxic effect of gamma-radiation in HeLa cells [8].…”

Section: Introductionmentioning

confidence: 99%

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He-Ne laser irradiation protects B-lymphoblasts from UVA-induced DNA damage

Dube¹,

Bock²,

Bauer³

et al. 2001

Radiation and Environmental Biophysics

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The effect of He-Ne laser (632.8 nm) pre-irradiation on UVA (343 nm)-induced DNA damage in the human B-lymphoblast cell line NC37 was investigated using the comet assay. He-Ne laser pre-irradiation was observed to result in a dose-dependent decrease in UVA-induced DNA damage. This effect was also found to be dependent on the incubation period between He-Ne laser pre-irradiation and the UVA exposure. Whereas the control cells with a higher DNA damage point to an initial ability of faster repair, both the control and the He-Ne laser pre-irradiated cells subsequently show the same rate of DNA repair. The results suggest that He-Ne laser irradiation protect the cells from UVA-induced DNA damage primarily through an influence on processes that prevent an initial DNA damage.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

He-Ne laser irradiation protects B-lymphoblasts from UVA-induced DNA damage

Dube¹,

Bock²,

Bauer³

et al. 2001

Radiation and Environmental Biophysics

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“…In this context, Leal Júnior et al [38] showed that phototherapy can delay the onset of fatigue, probably by local mechanisms, including minimizing oxidative stress. Studies have shown that infrared laser application before high-intensity exercise can increase the removal of blood lactate and reduce muscle damage, providing a faster muscle recovery [39,40]. …”

Section: Discussionmentioning

confidence: 99%

Effects of light-emitting diodes on muscle fatigue and exercise tolerance in patients with COPD: study protocol for a randomized controlled trial

Miranda¹,

Leal-Junior²,

Marchetti³

et al. 2013

Trials

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BackgroundLight-emitting diodes (LED) have been used to minimize muscle fatigue in athletes and healthy subjects. Patients with chronic obstructive pulmonary disease (COPD) are susceptible to early muscle fatigue.ObjectiveThe objective of this study is to investigate the acute effects of LED on muscle function, exercise capacity and cardiorespiratory responses during isometric and dynamic exercise in patients with COPD.MethodsThis study will assess 30 patients with moderate to severe obstruction (forced expiratory volume-one second,FEV1 ≤70% predicted). Isometric and dynamic protocols will be conducted in two visits each, for a total of four visits a week apart. First, venous blood will be taken from the patients. The isometric protocol will start with the determination of the maximum voluntary isometric contraction (MIVC) to determine the workload (60% of MIVC) for the isometric endurance test (IET). Patients will be randomized to receive either the placebo or LED application (each point will be irradiated for 30 s and the energy received at each point will be 41.7 J). Immediately after finishing this procedure, the patients will carry out the IET until the limit of tolerance or until a 20% fall of strength is observed. After the test, another blood draw will be taken. In another visit (one week later), the same order of procedures will be performed, except with the opposite (LED or placebo). For the dynamic endurance test (DET), the same procedures described above will be followed, except with 75% of the maximal workload obtained from the incremental cycle ergometer test used instead of the IET. The electromyography will be recorded during the isometric and dynamic protocols. Differences in muscle function, exercise capacity and cardiorespiratory responses between the LED and placebo applications will be analyzed. The therapeutic effects of LED could minimize muscle fatigue in patients with COPD by increasing exercise tolerance.Trial registrationTrial registration number: NCT01448564

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“…Vacca et al (1994) observaron que al irradiar templados de ADN, junto con enzimas de transcripción y traducción por separado en cultivo (2 joules/ cm 2 ), aumentó tanto la producción de ARN, como la producción de proteínas. Se comprobó también que el aumento de síntesis proteica ocurre tanto a nivel mitocondrial como citosólico (Vacca et al, 1996).…”

Section: Discussionunclassified

“…Entre los efectos observados en los tejidos biológi-cos, están el aumento en la producción de ATP intracelular por la estimulación mitocondrial, estímulo en la síntesis de ARN y proteínas (Greco et al, 1989;Vacca et al, 1994Vacca et al, , 1996 y la activación de canales para el intercambio iónico relacionado con un aumento de metabolismo celular (Karu, 1989). En otros estudios morfológicos, se ha observado un efecto bioestimulatorio en el tejido fibroconectivo (Vinck et al, 2003;Shu et al, 2002y Matamala et al, 2001.…”

Section: Introductionunclassified

Efecto Morfológico de la Irradiación Láser AsGa en el Músculo Esquelético de Rata

2007

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RESUMEN:La terapia láser AsGa es ampliamente utilizada en la rehabilitación de lesiones articulares y tendinosas. Por las características físicas del haz de luz utilizado, parte de esta irradiación es absorbida por tejido muscular adyacente, por lo que se planteó medir en un modelo experimental en rata, posibles efectos morfológicos de la irradiación láser AsGa en este tejido. Se realizó un estudio morfométrico y estereológico, utilizando ratas Sprague Dawley sanas, las que fueron irradiadas, por 10 días, en la zona del vientre del músculo gastrocnemio, utilizando tres diferentes dosis 0,8 (A1 y A2), 2 (B) y 5 (C) joules/cm 2 . Los miembros contralaterales no fueron irradiados (control).Se determinó el área de la fibra muscular por recuento de puntos (morfometría) en grupo A1. La densidad de volumen de tejido conectivo se midió a través un sistema test M42 (estereología) en los grupos A2, B y C.Los resultados obtenidos mostraron que el área de las fibras musculares fue significativamente mayor (p=0,001) en los músculos irradiados que en sus controles (1513±376 mm 2 -1146±286 mm 2 ). El análisis estereológico de la densidad de volumen de tejido conectivo (Vvtc) indicó que no existieron diferencias significativas (p=0,07) entre músculos irradiados y controles, en ninguna de las tres dosis utilizadas. Los resultados de este estudio indican que existe, a nivel morfológico, un efecto aparentemente hipertrófico del láser AsGa en el músculo esquelético de rata.

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Increase in cytosolic and mitochondrial protein synthesis in rat hepatocytes irradiated in vitro by HeNe laser

Cited by 30 publications

References 14 publications

He-Ne laser irradiation protects B-lymphoblasts from UVA-induced DNA damage

He-Ne laser irradiation protects B-lymphoblasts from UVA-induced DNA damage

Effects of light-emitting diodes on muscle fatigue and exercise tolerance in patients with COPD: study protocol for a randomized controlled trial

Efecto Morfológico de la Irradiación Láser AsGa en el Músculo Esquelético de Rata

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