Incorporation of Thymine Nucleotides by DNA Polymerases through T–Hg^II–T Base Pairing

Regulated Incorporation of Two Different Metal Ions into Programmed Sites in a Duplex by DNA Polymerase Catalyzed Primer Extension

“…By contrast, only the incorporation of dTTP or dCTP into the site opposite T, and not the incorporation of dTTP or dCTP opposite C, proceeded in the presence of Hg II ions (Figure 4 b). [11] These results indicate that the incorporation of dTTP into the site opposite C in the template is highly specific to Ag I ions. [11] These results indicate that the incorporation of dTTP into the site opposite C in the template is highly specific to Ag I ions.…”

“…[4] Metalmediated base pairs have thus attracted considerable interest in the field of nanodevices, for example, in the context of electronic wires, [5] magnetic devices, [6] and DNA-based logic gates. [11] A metal-mediated base pair consisting of an artificial base was reported to be recognized by DNA polymerase as well. [11] A metal-mediated base pair consisting of an artificial base was reported to be recognized by DNA polymerase as well.…”

Wie entsteht Leben: Ein altes Problem gebiert neue Chemie

“…[14][15][16][17] Zunächst einmal kann man eine neue Perspektive gewinnen, indem man Probleme aus ferner Vergangenheit abstrahiert und sie im Kontext zukünftiger Technologien und synthetischer Zellen betrachtet. Außerdem wurden in den letzten Jahren immer häufiger alternative biochemische Wege beschritten, bei denen der genetische Code um neuartige replizierbare Basenpaare, [20][21][22] Wechselwirkungen zwischen Strängen [23][24][25] und Rückgratverknüpfungen [26][27][28] erweitert wurde. Diese Modelle liefern Denkanstçße für neue Ideen, die experimentell überprüft werden kçnnen.…”

Metal ion-directed dynamic splicing of DNA through global conformational change by intramolecular complexation

“…For example, two thymines and cytosines coordinate to Hg 2 þ and Ag þ , respectively, to form a M-DNA (metal ion DNA complex) duplex through the formation of metal-centred nucleobase dimers 7,8,[12][13][14][15] . A silver ion can be accommodated in the nucleobase triplet CGC of a DNA triplex by substitution of the N3 proton of cytosine on the third strand, which stabilizes the triplex structure even under neutral pH 10,11 .…”