Silver turns up the A-C: In the presence of Ag(I) ions, a DNA polymerase incorporated deoxyadenosine (from dATP) at the site opposite cytosine in the template strand to afford the full-length product (see scheme), meaning that DNA polymerases prefer a C-Ag(I)-A base pair to the more thermodynamically stable C-Ag(I)-C base pair.
A dangerous matchmaker: In the presence of HgII ions, DNA polymerases incorporated thymidine 5′‐triphosphate (TTP) at the site opposite thymine in a template strand and made a phosphodiester bond to elongate the primer strand. This unusual metal‐mediated base pair was recognized by the DNA polymerases, which went on to synthesize the full‐length product (see picture).
Metal-mediated base pairs formed by the coordination of metal ions to natural or artificial bases impart unique chemical and physical properties to nucleic acids and have attracted considerable interest in the field of nanodevices. Ag(I) ions were found to mediate DNA polymerase catalyzed primer extension through the formation of a C-Ag(I)-T base pair, as well as the previously reported C-Ag(I)-A base pair. The comparative susceptibility of dNTPs to Ag(I)-mediated enzymatic incorporation into the site opposite cytosine in the template was shown to be dATP>dTTP≫dCTP. Furthermore, two kinds of metal ions, Ag(I) and Hg(II), selectively mediate the incorporation of thymidine 5'-triphosphate into sites opposite cytosine and thymine in the template, respectively. In other words, the regulated incorporation of different metal ions into programmed sites in the duplex by DNA polymerase was successfully achieved.
Mit Silber zu A–C: In Gegenwart von AgI‐Ionen baut eine DNA‐Polymerase Desoxyadenosin (aus dATP) gegenüber einem Cytosinrest im Templatstrang unter Bildung des Volllängenprodukts ein (siehe Schema). Das bedeutet, dass DNA‐Polymerasen ein C‐AgI‐A‐Basenpaar dem thermodynamisch stabileren C‐AgI‐C‐Basenpaar vorziehen.
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