Incorporation of silver nanoparticles on the surface of orthodontic microimplants to achieve antimicrobial properties

Venugopal, Adith; Muthuchamy, Nallal; Tejani, Harsh; Gopalan, A.; Lee, Heon-Jin; Kyung, Hee-Moon

doi:10.4041/kjod.2017.47.1.3

Cited by 42 publications

(26 citation statements)

References 31 publications

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“…Venugopal et al reported that 10~30 nm AgNPs had no statistical cytotoxicity. 46 In this study, we fabricated the AgNPs from Ag+ by the method of photocatalytic reduction, and the size of AgNPs coated on the TiO 2 -NTs layer was satisfied with around 20nm detected by TEM images ( Figure 1B). 9,11 For this method, the concentration of the silver salt plays a very important role for both the size and the shape of AgNPs which can be regulated by changing the concentration of AgNO 3 solution.…”

Section: Results and Discussion Surface Characterization And Ag + Relmentioning

confidence: 76%

Improved Immunoregulation of Ultra-Low-Dose Silver Nanoparticle-Loaded TiO2 Nanotubes via M2 Macrophage Polarization by Regulating GLUT1 and Autophagy

Chen¹,

Guan²,

Ren³

et al. 2020

IJN

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Introduction: The bone regeneration of endosseous implanted biomaterials is often impaired by the host immune response, especially macrophage-related inflammation which plays an important role in the bone healing process. Thus, it is a promising strategy to design an osteo-immunomodulatory biomaterial to take advantage of the macrophage-related immune response and improve the osseointegration performance of the implant. Methods: In this study, we developed an antibacterial silver nanoparticle-loaded TiO 2 nanotubes (Ag@TiO 2-NTs) using an electrochemical anodization method to make the surface modification and investigated the influences of Ag@TiO 2-NTs on the macrophage polarization, osteo-immune microenvironment as well as its potential molecular mechanisms in vitro and in vivo. Results: The results showed that Ag@TiO 2-NTs with controlled releasing of ultra-low-dose Ag + ions had the excellent ability to induce the macrophage polarization towards the M2 phenotype and create a suitable osteo-immune microenvironment in vitro, via inhibiting PI3K/Akt, suppressing the downstream effector GLUT1, and activating autophagy. Moreover, Ag@TiO 2-NTs surface could improve bone formation, suppress inflammation, and promote osteo-immune microenvironment compared to the TiO 2-NTs and polished Ti surfaces in vivo. These findings suggested that Ag@TiO 2-NTs with controlled releasing of ultra-low-dose Ag + ions could not only inhibit the inflammation process but also promote the bone healing by inducing healing-associated M2 polarization. Discussion: Using this surface modification strategy to modulate the macrophage-related immune response, rather than prevent the host response, maybe a promising strategy for implant surgeries in the future.

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Section: Results and Discussion Surface Characterization And Ag + Relmentioning

confidence: 76%

Improved Immunoregulation of Ultra-Low-Dose Silver Nanoparticle-Loaded TiO2 Nanotubes via M2 Macrophage Polarization by Regulating GLUT1 and Autophagy

Chen¹,

Guan²,

Ren³

et al. 2020

IJN

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“…Researches indicated that 5-50 nm silver nanosilver particles have antimicrobial properties against microorganisms 32 and that 10-30 nm nanosilver particles effectively suppress pathogens and show no statistically cytotoxicity. 39 In this study, 20-30 nm diameter spherical nanosilver particles were utilized. Several polymers have been used to stabilize nanosilver particles because of their tendency to agglomerate and form large clusters, including polyallylamine, 40 polyethylenimine, 41 chitosan, 42 and poly (vinyl-pyrrolidone).…”

Section: Discussionmentioning

confidence: 99%

Nanosilver/poly (DL-lactic-co-glycolic acid) on titanium implant surfaces for the enhancement of antibacterial properties and osteoinductivity

Zeng

Xiong

Zhuo

et al. 2019

IJN

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Background: Despite titanium (Ti) implants have been commonly used in the medical device field due to their superior biocompatibility, implant-associated bacterial infection remains a major clinical complication. Nanosilver, an effective antibacterial agent against a wide spectrum of bacterial strains, with a low-resistance potential, has attracted much interest too. Incorporation of nanosilver on Ti implants may be a promising approach to prevent biofilm formation. Purpose: The objective of the study was to investigate the antibacterial effects and osteoinductive properties of nanosilver/poly (dl-lactic-co-glycolic acid)-coated titanium (NSPTi). Methods: Gram-positive methicillin-resistant Staphylococcus aureus (MRSA) and the Gramnegative opportunistic pathogen Pseudomonas aeruginosa (PAO-1) were used to evaluate the antibacterial activity of NSPTi implants through the analysis of bacterial colonization in vitro and in vivo. Furthermore, we examined the osteoinductive potential of NSPTi implants by investigating the proliferation and differentiation of MC3T3-E1 preosteoblast cells. In vivo, the osteoinductive properties of NSPTi implants were assessed by radiographic evaluation, H&E staining, and Masson's trichrome staining. Results: In vitro, bacterial adhesion to the 2% NSPTi was significantly inhibited and ,1% of adhered bacteria survived after 24 hours. In vitro, the average colony-forming units (CFU)/g ratios in the 2% NSPTi with 10 3 CFU MRSA and PAO-1 were 1.50±0.68 and 1.75±0.6, respectively. In the uncoated Ti groups, the ratios were 1.03±0.82×10 3 and 0.94±0.49×10 3 , respectively. These results demonstrated that NSPTi implants had prominent antibacterial properties. Proliferation of MC3T3-E1 cells on the 2% NSPTi sample was 1.51, 1.78, and 2.22 times that on the uncoated Ti control after 3, 5, and 7 days' incubation, respectively. Furthermore, NSPTi implants promoted the maturation and differentiation of MC3T3-E1 cells. In vivo, NSPTi accelerated the formation of new bone while suppressing bacterial survival. Conclusion: NSPTi implants have simultaneous antibacterial and osteoinductive activities and therefore have the potential in clinical applications.

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“…Although our results might support this possible argument, more studies that determine the effect of AgNPs according to the corrosion level, topographic alterations, roughness, ion release, and other important properties of the most used orthodontic archwires should be investigated. In addition, the AgNPs have also been used for other different applications in the orthodontic field; the results of those investigations demonstrate that the orthodontic appliances with AgNPs have the ability to improve their antibacterial properties in several types of microorganisms involved in the white spots [36][37][38][39]. Our results suggest that the AgNP samples used in this study have the potential to be an excellent antimicrobial alternative with antiadherence properties that might facilitate the prevention of white spots caused by the S. mutans bacteria during the orthodontic therapeutics for the most conventional orthodontic appliances.…”

Section: Discussionmentioning

confidence: 99%

Antiadherence and Antimicrobial Properties of Silver Nanoparticles against Streptococcus mutans on Brackets and Wires Used for Orthodontic Treatments

Espinosa-Cristóbal

López-Ruiz

Cabada-Tarín

et al. 2018

Journal of Nanomaterials

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White spot lesions (WSLs) are very frequent alterations during orthodontic treatments causing demineralization of the dental enamel. Various dental treatments have been developed to prevent WSLs; the prevalence and incidence of these lesions remain significantly high. Although silver nanoparticles (AgNPs) have demonstrated good inhibitory effects against several microorganisms, more studies about antiadherence activity on different orthodontic appliance surfaces are necessary. To determine the inhibitory effect and antiadherence activity of AgNPs on the adhesion of S. mutans on surfaces of brackets and wires for orthodontic therapies, two sizes of AgNPs were prepared and characterized. The evaluation of S. mutans adhesion was performed with microbiological assays on surfaces of brackets and orthodontic modules in triplicate. Topographic characteristics of orthodontic brackets and wires were made by scanning electron and atomic force microscopies. All AgNP samples inhibited S. mutans adhesion; however, the smaller AgNPs had better inhibition than the larger ones. The presence of the module influenced the adhesion of S. mutans but not in the activity of AgNPs. The AgNPs used in this study showed to have good antimicrobial and antiadherence properties against S. mutans bacteria determining its high potential use for the control of WSLs in orthodontic treatments.

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Incorporation of silver nanoparticles on the surface of orthodontic microimplants to achieve antimicrobial properties

Cited by 42 publications

References 31 publications

<p>Improved Immunoregulation of Ultra-Low-Dose Silver Nanoparticle-Loaded TiO<sub>2</sub> Nanotubes via M2 Macrophage Polarization by Regulating GLUT1 and Autophagy</p>

<p>Improved Immunoregulation of Ultra-Low-Dose Silver Nanoparticle-Loaded TiO<sub>2</sub> Nanotubes via M2 Macrophage Polarization by Regulating GLUT1 and Autophagy</p>

<p>Nanosilver/poly (DL-lactic-co-glycolic acid) on titanium implant surfaces for the enhancement of antibacterial properties and osteoinductivity</p>

Antiadherence and Antimicrobial Properties of Silver Nanoparticles against Streptococcus mutans on Brackets and Wires Used for Orthodontic Treatments

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