Incorporation of partially purified hen egg white lysozyme into zein films for antimicrobial food packaging

Mecitoğlu, Çiğdem; Yemenicioğlu, Ahmet; Arslanoğlu, Alper; Elmacı, Zehra Seda; Korel, Figen; Çetin, Ali Emrah

doi:10.1016/j.foodres.2005.05.007

Cited by 128 publications

(88 citation statements)

References 24 publications

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“…Table 3 shows the application area when lysozyme is used as an antimicrobial agent and when packaging materials are used in antimicrobial food packaging. Several studies have shown that incorporating lysozyme as a preservative in packaging materials extends the shelf life of foods (Appendini & Hotchkiss, 1997;Buonocore et al, 2004;Mecitoglu et al, 2006). Food-borne microbial outbreaks have garnered much of the media's attention and as such have sparked a greater interest in antimicrobial food packaging.…”

Section: Lysozyme In Food Packagingmentioning

confidence: 99%

“…For instance, the combination of lysozyme with EDTA makes lysozyme highly effective on Gram-negative bacteria (Mecitoglu et al, 2006), and conjugates of lysozyme with dextran, galactomannan or xyloglucan have good antimicrobial activity against both Gram-positive and Gram-negative bacteria when applied in combination with mild heating at 50°C (Nakamura et al, 1992).…”

Section: Lysozyme In Food Packagingmentioning

confidence: 99%

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Discovery, Modification and Production of T4 Lysozyme for Industrial and Medical Uses

Alhazmi¹,

Stevenson²,

Amartey³

et al. 2014

IJB

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Lysozyme has attracted immense attention as an antimicrobial agent because of its ability to lyse the bacterial cell wall. It is found in a wide variety of body fluids and in cells of the innate immune system. Lysozyme can act as muramidase or as a Cationic Antimicrobial Peptide (CAMP). Lysozyme has many applications in the medical and industrial fields. Based on enzyme nomenclature, lysozyme is classified as a glycosylase under the group hydrolases. This manuscript covers a fundamental review of lysozyme in terms of discovery, history, functions and various sources and types of lysozyme. The biological and molecular structure is discussed as well as notable bioengineering and protein modifications. Furthermore, the mechanisms of resistance to lysozyme in microorganisms have also been discussed. Lastly, different methods that have been developed for detecting and measuring the activity of lysozyme are outlined. Although, a recombinant lysozyme has not yet been produced, several studies have attempted to generate a modified lysozyme either for large-scale production or that which is more suitable for industrialization purposes.

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Section: Lysozyme In Food Packagingmentioning

confidence: 99%

Section: Lysozyme In Food Packagingmentioning

confidence: 99%

Discovery, Modification and Production of T4 Lysozyme for Industrial and Medical Uses

Alhazmi¹,

Stevenson²,

Amartey³

et al. 2014

IJB

View full text Add to dashboard Cite

show abstract

“…After 6 h at room temperature, the mixture was centrifuged at 15, 000 Â g for 15 min at 4°C and the precipitate was discarded. For preparation of lysozyme, the supernatant containing the enzyme was first dialyzed for 21 h at 4°C against three changes of 2 L distilled water and then lyophilized by using a freeze drier (Labconco, FreeZone, 6 l, Kansas City, MO, USA) (Mecitoglu et al, 2006). The lyophilized enzyme was stored at À18°C and its activity was determined as U/mg before each film preparation.…”

Section: Production Of Lysozymementioning

confidence: 99%

“…The activity of soluble lysozyme was determined as given in Mecitoglu et al (2006) by measuring the decrease in absorbance of M. lysodeikticus suspension (0.26 mg/ml) prepared in 0.05 M Na-phosphate buffer at pH 7.0. A volume of 2.3 ml of the substrate was mixed with 0.2 ml of lysozyme solution.…”

Section: Determination Of Soluble Lysozyme Activitymentioning

confidence: 99%

“…The immobilized lysozyme activity was determined as described by Mecitoglu et al (2006). The films used in release studies were cut into 2 pieces, placed into glass Petri dishes and brought into contact with 25 ml M. lysodeikticus suspension (0.26 mg/ml) at 30°C prepared in 0.05 M Na-phosphate buffer at pH 7.0.…”

Section: Determination Of the Immobilized Lysozyme Activity Retained mentioning

confidence: 99%

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Development of cellulose acetate based antimicrobial food packaging materials for controlled release of lysozyme

Gemili

Yemenicioğlu

Altınkaya

2009

Journal of Food Engineering

Self Cite

180

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a b s t r a c tAntimicrobial packaging materials were obtained by incorporation of lysozyme into cellulose acetate (CA) films. In order to achieve controlled release of lysozyme, the structure of the films was changed from highly asymmetric and porous to dense by modulating the composition of the initial casting solution. The highest release rate, soluble lysozyme activity and antimicrobial activity were obtained with the film prepared from 5% CA solution including 1.5% lysozyme. Increasing CA content in the casting solution decreased the porosity of the films, hence, reduced the release rate, maximum released lysozyme activities and the antimicrobial activities of the films. In contrast, immobilized lysozyme activities and the tensile strength of the films increased. The incorporation of lysozyme did not cause significant reductions in tensile strength and elongation at break values except in films prepared with 15% CA. This study showed the good potential of asymmetric CA films to achieve controlled release in antimicrobial packaging.

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Antimicrobial Enzymes and Natural Extracts in Plastics

Mastromatteo

Conte

Nobile

2011

Antimicrobial Polymers

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Incorporation of partially purified hen egg white lysozyme into zein films for antimicrobial food packaging

Cited by 128 publications

References 24 publications

Discovery, Modification and Production of T4 Lysozyme for Industrial and Medical Uses

Discovery, Modification and Production of T4 Lysozyme for Industrial and Medical Uses

Development of cellulose acetate based antimicrobial food packaging materials for controlled release of lysozyme

Antimicrobial Enzymes and Natural Extracts in Plastics

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