Incorporation of ovalbumin into ISCOMs and related colloidal particles prepared by the lipid film hydration method

Evaluation of ISCOM matrices clearance from rabbit nasal cavity by gamma scintigraphy

“…The size of the particles obtained was in the range of 42-67 nm as measured by photon correlation spectroscopy with polydispersity index of 0.216. These results are in agreements with other reports (Demana et al, 2004;Lendemans et al, 2005) demonstrating that lipid hydration method produces more heterogeneous formulation as compare to dialysis method. Table 1 Stability of 99m Tc labelled ISCOM matrices formulations.…”

“…ISCOM matrices were prepared by lipid hydration method (Demana et al, 2004). Briefly, 8 mg PC and 4 mg cholesterol were dissolved in 1 ml chloroform.…”

Effect of incorporation of the adjuvant Quil A on structure and immune stimulatory capacity of liposomes

“…Ovalbumin (OVA; Grade V, purity approx. 98%, Sigma‐Aldrich Pty Ltd, USA) was conjugated to fluorescein isothiocyanate (fitc; Sigma‐Aldrich, USA) and phosphatidylethanolamine (PE; Sigma‐Aldrich) using a previously described method 16 , 19 . Briefly, 100 mg of OVA was added to a solution of 20 mg of fitc in 10 mL of 20 mmol/L carbonate buffer (pH 9.5).…”

“…Inclusion of Quil A disrupts the lipid bilayer structure of the liposomes causing a variety of particles to be formed depending on the ratios of phospholipids, cholesterol and Quil A used. In previous studies we have constructed pseudo‐ternary phase diagrams to characterize the structures produced 16 , 17 , 18 . A number of different colloidal structures are produced including classical ISCOM‐like particles, ring‐like micelles, worm‐like micelles, lamellae (hexagonal arrays of ring‐like micelles) and lipidic/layered structures.…”

Immunogenicity of Liposomes Containing Lipid Core Peptides and the Adjuvant Quil A

“…Liposomes, on the other hand, have a demonstrated safety profile in both vaccine and drug formulations (19). They also possess the ability to entrap a wide variety of potential antigens, thereby increasing their generic utility (20).…”