Incorporation of graphene oxide into poly(ɛ-caprolactone) 3D printed fibrous scaffolds improves their antimicrobial properties

Melo, Sofia Ferreira; Neves, Sara C.; Pereira, Andreia; Borges, Inês; Granja, Pedro L.; Magalhães, Fernão D.; Gonçalves, Inês C.

doi:10.1016/j.msec.2019.110537

Cited by 32 publications

(40 citation statements)

References 73 publications

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“…Alternate materials to antibiotics are now being explored, particularly carbon-derived materials, such as graphene-based materials (GBMs), and they have started to receive increased attention owing to their potential antibacterial properties. Melo et al [ 53 ] incorporated graphene oxide (GO) to develop a polycaprolactone (PCL) fibrous-based scaffold because smaller oxidized versions of GBM’s have been reported to have a higher biocompatibility with cells. GO was dispersed in PCL using suitable solvents.…”

Section: Techniques and Effectiveness Of Developed Antibacterial 3d-printed Materialsmentioning

confidence: 99%

Embracing Additive Manufacturing Technology through Fused Filament Fabrication for Antimicrobial with Enhanced Formulated Materials

2021

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Antimicrobial materials produced by 3D Printing technology are very beneficial, especially for biomedical applications. Antimicrobial surfaces specifically with enhanced antibacterial property have been prepared using several quaternary salt-based agents, such as quaternary ammonium salts and metallic nanoparticles (NPs), such as copper and zinc, which are incorporated into a polymeric matrix mainly through copolymerization grafting and ionic exchange. This review compared different materials for their effectiveness in providing antimicrobial properties on surfaces. This study will help researchers choose the most suitable method of developing antimicrobial surfaces with the highest efficiency, which can be applied to develop products compatible with 3D Printing Technology.

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Section: Techniques and Effectiveness Of Developed Antibacterial 3d-printed Materialsmentioning

confidence: 99%

Embracing Additive Manufacturing Technology through Fused Filament Fabrication for Antimicrobial with Enhanced Formulated Materials

2021

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“…In particular, methicillin-sensitive S. aureus (MSSA), CNS, and methicillin-resistant S. aureus (MRSA) have percentages of about 35, 23, and 15%, respectively. The most frequently identified CNS species is Staphylococcus epidermidis (67.8%) [ 1 , 8 , 9 , 19 , 20 ]. Other Gram-positive bacteria ( Enterococcus spp., Streptococcus spp., Clostridium difficile ) account for 11.1%, and Gram-negative bacteria ( Pseudomonas aeruginosa , Enterobacteriaceae, Proteus spp.)…”

Section: Introductionmentioning

confidence: 99%

“…Other Gram-positive bacteria ( Enterococcus spp., Streptococcus spp., Clostridium difficile ) account for 11.1%, and Gram-negative bacteria ( Pseudomonas aeruginosa , Enterobacteriaceae, Proteus spp.) account for 2.5% of total infections [ 8 , 20 ]. Hence, as a general guide, the antibiotic choice should at least provide adequate coverage against S. aureus (including MRSA), CNS, and aerobic Gram-negative bacilli [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Silver-Functionalized Poly(ε-Caprolactone)/Biphasic Calcium Phosphate Scaffolds Designed to Counteract Post-Surgical Infections in Orthopedic Applications

Comini

Sparti

Coppola

et al. 2021

IJMS

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In this study, we designed and developed novel poly(ε-caprolactone) (PCL)-based biomaterials, for use as bone scaffolds, through modification with both biphasic calcium phosphate (BCP), to impart bioactive/bioresorbable properties, and with silver nitrate, to provide antibacterial protection against Staphylococcus aureus, a microorganism involved in prosthetic joint infections (PJIs). Field emission scanning electron microscopy (FESEM) showed that the samples were characterized by square-shaped macropores, and energy dispersive X-ray spectroscopy analysis confirmed the presence of PCL and BCP phases, while inductively coupled plasma–mass spectrometry (ICP–MS) established the release of Ag+ in the medium (~0.15–0.8 wt% of initial Ag content). Adhesion assays revealed a significant (p < 0.0001) reduction in both adherent and planktonic staphylococci on the Ag-functionalized biomaterials, and the presence of an inhibition halo confirmed Ag release from enriched samples. To assess the potential outcome in promoting bone integration, preliminary tests on sarcoma osteogenic-2 (Saos-2) cells indicated PCL and BCP/PCL biocompatibility, but a reduction in viability was observed for Ag-added biomaterials. Due to their combined biodegrading and antimicrobial properties, the silver-enriched BCP/PCL-based scaffolds showed good potential for engineering of bone tissue and for reducing PJIs as a microbial anti-adhesive tool used in the delivery of targeted antimicrobial molecules, even if the amount of silver needs to be tuned to improve osteointegration.

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“…Both biological and mechanical properties of the matrix were improved on the addition of GO. Melo et al [ 260 ] and Unagolla et al [ 261 ] reported improvement in the antimicrobial properties and the enhancement of cellular response. Melo et al found an 80% increase in bacterial death after 24 h in contact with a PCL/GO scaffold.…”

Section: 3d Printed Scaffolds With Carbon-based Nanomaterialsmentioning

confidence: 99%

“…Surface roughness and irregularity increased with the addition of GO. The average diameter of the fibres did not change with GO (reprinted from [ 260 ], with permission from Elsevier).…”

Section: Figurementioning

confidence: 99%

Advances in Biodegradable 3D Printed Scaffolds with Carbon-Based Nanomaterials for Bone Regeneration

et al. 2020

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Bone possesses an inherent capacity to fix itself. However, when a defect larger than a critical size appears, external solutions must be applied. Traditionally, an autograft has been the most used solution in these situations. However, it presents some issues such as donor-site morbidity. In this context, porous biodegradable scaffolds have emerged as an interesting solution. They act as external support for cell growth and degrade when the defect is repaired. For an adequate performance, these scaffolds must meet specific requirements: biocompatibility, interconnected porosity, mechanical properties and biodegradability. To obtain the required porosity, many methods have conventionally been used (e.g., electrospinning, freeze-drying and salt-leaching). However, from the development of additive manufacturing methods a promising solution for this application has been proposed since such methods allow the complete customisation and control of scaffold geometry and porosity. Furthermore, carbon-based nanomaterials present the potential to impart osteoconductivity and antimicrobial properties and reinforce the matrix from a mechanical perspective. These properties make them ideal for use as nanomaterials to improve the properties and performance of scaffolds for bone tissue engineering. This work explores the potential research opportunities and challenges of 3D printed biodegradable composite-based scaffolds containing carbon-based nanomaterials for bone tissue engineering applications.

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Incorporation of graphene oxide into poly(ɛ-caprolactone) 3D printed fibrous scaffolds improves their antimicrobial properties

Cited by 32 publications

References 73 publications

Embracing Additive Manufacturing Technology through Fused Filament Fabrication for Antimicrobial with Enhanced Formulated Materials

Embracing Additive Manufacturing Technology through Fused Filament Fabrication for Antimicrobial with Enhanced Formulated Materials

Novel Silver-Functionalized Poly(ε-Caprolactone)/Biphasic Calcium Phosphate Scaffolds Designed to Counteract Post-Surgical Infections in Orthopedic Applications

Advances in Biodegradable 3D Printed Scaffolds with Carbon-Based Nanomaterials for Bone Regeneration

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