Incorporation of Fucoidan in β-Tricalcium phosphate-Chitosan scaffold prompts the differentiation of human bone marrow stromal cells into osteogenic lineage

Abstract

In our previous study, we reported the fabrication and characterization of a novel tricalcium phosphatefucoidan-chitosan (TCP-Fu-Ch) biocomposite scaffold. However, the previous report did not show whether the biocomposite scaffold can exhibit osteogenic differentiation of human bone marrow stromal cells in osteogenic media and normal media supplemented with platelet-derived growth factor (PDGF-BB). On day 15, the release of osteocalcin, was significant in the TCP-Fu-Ch scaffold, when compared with that in the TCP-Ch scaffold, and the level of release was approximately 8 and 6 ng/ml in osteogenic and normal media supplemented with PDGF-BB, respectively. Scanning electron microscopy of the TCP-Fu-Ch scaffold demonstrated mineralization and apatite layer formation on day 14, while the addition of PDGF-BB also improved the osteogenic differentiation of the scaffold. An array of gene expression analysis demonstrated that TCP-Fu-Ch scaffold cultured in osteogenic and normal media supplemented with PDGF-BB showed significant improvement in the expression of collagen 1, Runt-related transcription factor 2, osteonectin, bone gamma-carboxyglutamate protein, alkaline phosphatase, and PPA2, but a decline in the expression of integrin. Altogether, the present study demonstrated that fucoidan-incorporated TCP-Ch scaffold could be used in the differentiation of bone marrow stromal cells and can be a potential candidate for the treatment of bone-related ailments through tissue engineering technology.

Marine Polysaccharides in Pharmaceutical Applications: Fucoidan and Chitosan as Key Players in the Drug Delivery Match Field

“…Fucoidan has important properties for artificial bone development, namely, inducing osteogenic genes and enhancing febrile collagen matrix formation, while chitosan can be used to modify cell adhesion, proliferation, and differentiation. Scaffolds composed of fucoidan and chitosan have been established as biomaterials for bone regeneration [141][142][143][144][145][146]. Likewise, several examples of hydrogels based on fucoidan and chitosan can be found in the literature developed mainly as a solution for wound healing [144,[147][148][149][150].…”

Incorporation of Human-Platelet-Derived Growth Factor-BB Encapsulated Poly(lactic-co-glycolic acid) Microspheres into 3D CORAGRAF Enhances Osteogenic Differentiation of Mesenchymal Stromal Cells

“…To examine the level of osteogenic differentiation, gene xpression was employed according to a previously established method. 36…”

Preparation, Characterization and Biological Applications of Biosynthesized Silver Nanoparticles with Chitosan-Fucoidan Coating

“…Chitosan and fucoidan are oppositely charged polysaccharides, thus enabling the formation of polyelectrolyte complexes via self-assembly [ 31 , 32 , 33 ]. Several studies have suggested that chitosan–fucoidan polyelectrolyte/nanoparticles have great advantages in drug delivery [ 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 ] and tissue engineering [ 40 , 41 , 42 ] due to their efficient drug encapsulation and cellular uptake.…”