Incorporation of fast dissolving glucose porogens into an injectable calcium phosphate cement for bone tissue engineering

Smith, Brandon T.; Santoro, Marco; Grosfeld, Eline C.; Shah, Sarita R.; Beucken, Jeroen J.J.P. van den; Jansen, John A.; Mikos, Antonios G.

doi:10.1016/j.actbio.2016.12.024

Cited by 44 publications

(51 citation statements)

References 33 publications

(44 reference statements)

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“…All experimental groups showed a decrease in mass following immersion in PBS. Theoretically, 19.7 wt% of the samples’ mass can be attributed to glucose that has precipitated out of the 24 wt% Na 2 HPO 4 solution [16]. The G0P0 composite retained 82.2 ± 1.8% of its initial weight after 3 days, which is in accordance with the theoretical amount of glucose that precipitates out of the saturated liquid.…”

Section: Resultssupporting

confidence: 75%

“…The glucose microparticles (GMPs) were prepared as previously described [16]. Briefly, 40 g of > 99.5% glucose crystals (D-(+)-glucose (Sigma-Aldrich, St. Louis, MO)) were mixed with 15 mL deionized water (diH2O) to create a glucose slurry.…”

Section: Methodsmentioning

confidence: 99%

“…Following previously established protocols, composite porosity was evaluated by microcomputed tomography (μCT) [16,26,27]. In brief, samples at 3 days, 4 wks, and 8 wks were scanned at a current of 250 mA and a voltage of 40 kV with no filter using a SkyScan 1172 μCT imaging system (SkyScan, Aartselaar, Belgium).…”

Section: Methodsmentioning

confidence: 99%

“…In this method, a mixture of the CPC and porogen is cast in a mold, allowed to dry, and then the porogen is leached, generating the pores [15]. Glucose and poly(lactic-co-glycolic acid) (PLGA) have both been widely studied as potential additives to CPCs to generate porosity by this method [11,12,16]. Glucose rapidly dissolves in an aqueous solution, which allows the creation of porosity within a matter of minutes when incorporated within CPCs [16,17].…”

Section: Introductionmentioning

confidence: 99%

“…Glucose and poly(lactic-co-glycolic acid) (PLGA) have both been widely studied as potential additives to CPCs to generate porosity by this method [11,12,16]. Glucose rapidly dissolves in an aqueous solution, which allows the creation of porosity within a matter of minutes when incorporated within CPCs [16,17]. Glucose is also efficiently processed by cells with no harmful degradation byproducts [18].…”

Section: Introductionmentioning

confidence: 99%

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Incorporation of fast dissolving glucose porogens and poly(lactic-co-glycolic acid) microparticles within calcium phosphate cements for bone tissue regeneration

Smith

Watson

et al. 2018

Acta Biomaterialia

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A multitude of strategies and techniques have been investigated for the introduction of macropores with calcium phosphate cements (CPC). However, many of these strategies take several weeks to months to generate a maximal porosity or the degradation products of the porogen can trigger a localized inflammatory response in vivo. As such, it was hypothesized that the fast dissolution of glucose microparticles (GMPs) in a CPC composite also incorporating poly(lactic-co-glycolic acid) (PLGA) microparticles (MPs) will create an initial macroporosity and increase the surface area within the CPC, thus enhancing the diffusion of PLGA degradation products and preventing a significant decrease in pH. Furthermore, as PLGA degradation occurs over several weeks to months, additional macroporosity will be generated at later time points within CPCs. The results offer a new method for generating macroporosity in a multimodal fashion that also mitigates the effects of acidic degradation products.

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Section: Resultssupporting

confidence: 75%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Incorporation of fast dissolving glucose porogens and poly(lactic-co-glycolic acid) microparticles within calcium phosphate cements for bone tissue regeneration

Smith

Watson

et al. 2018

Acta Biomaterialia

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Advances in 3D printing of composite scaffolds for the repairment of bone tissue associated defects

Anandhapadman¹,

Venkateswaran²,

Jayaraman³

et al. 2022

Biotechnology Progress

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The conventional methods of using autografts and allografts for repairing defects in bone, the osteochondral bone, and the cartilage tissue have many disadvantages, like donor site morbidity and shortage of donors. Moreover, only 30% of the implanted grafts are shown to be successful in treating the defects. Hence, exploring alternative techniques such as tissue engineering to treat bone tissue associated defects is promising as it eliminates the above‐mentioned limitations. To enhance the mechanical and biological properties of the tissue engineered product, it is essential to fabricate the scaffold used in tissue engineering by the combination of various biomaterials. Three‐dimensional (3D) printing, with its ability to print composite materials and with complex geometry seems to have a huge potential in scaffold fabrication technique for engineering bone associated tissues. This review summarizes the recent applications and future perspectives of 3D printing technologies in the fabrication of composite scaffolds used in bone, osteochondral, and cartilage tissue engineering. Key developments in the field of 3D printing technologies involves the incorporation of various biomaterials and cells in printing composite scaffolds mimicking physiologically relevant complex geometry and gradient porosity. Much recently, the emerging trend of printing smart scaffolds which can respond to external stimulus such as temperature, pH and magnetic field, known as 4D printing is gaining immense popularity and can be considered as the future of 3D printing applications in the field of tissue engineering.

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Multimodal pore formation in calcium phosphate cements

Lodoso‐Torrecilla

Gestel

Díaz-Gómez

et al. 2017

J Biomedical Materials Res

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Calcium phosphate cements (CPCs) are commonly used as bone substitute materials. However, their slow degradation rate and lack of macroporosity hinders new bone formation. Poly(dl-lactic-co-glycolic acid) (PLGA) incorporation is of great interest as, upon degradation, produces acidic by-products that enhance CPC degradation. Yet, new bone formation is delayed until PLGA degradation occurs a few weeks after implantation. Therefore, the aim of this study was to accelerate the early stage pore formation within CPCs in vitro. With that purpose, we incorporated the water-soluble porogen sucrose at different weight percentages (10 or 20 wt %) to CPC and CPC/PLGA composites. The results revealed that incorporation of sucrose porogens increased mass loss within the first week of in vitro degradation in groups containing sucrose compared to control groups. After week 1, a further mass loss was observed related to PLGA and CPC degradation. Macroporosity analysis confirmed that macroporosity formation is influenced by the dissolution of sucrose at an early stage and by the degradation of PLGA and CPC at a later stage. We concluded that the combination of sucrose and PLGA porogens in CPC is a promising approach to promote early stage bone tissue ingrowth and complete replacement of CPC through multimodal pore formation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 500-509, 2018.

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Incorporation of fast dissolving glucose porogens into an injectable calcium phosphate cement for bone tissue engineering

Cited by 44 publications

References 33 publications

Incorporation of fast dissolving glucose porogens and poly(lactic-co-glycolic acid) microparticles within calcium phosphate cements for bone tissue regeneration

Incorporation of fast dissolving glucose porogens and poly(lactic-co-glycolic acid) microparticles within calcium phosphate cements for bone tissue regeneration

Advances in 3D printing of composite scaffolds for the repairment of bone tissue associated defects

Multimodal pore formation in calcium phosphate cements

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