Incorporation of capillary microsampling into whole body plethysmography and modified Irwin safety pharmacology studies in rats

Prior, Helen; Marks, Louise; Grant, Claire; South, Marie C.

doi:10.1016/j.yrtph.2015.06.002

Cited by 7 publications

(2 citation statements)

References 24 publications

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“…CMS provides an automated handling tool with less time for stabilization, which offers a new opportunity for labile analytes. As a technique that can provide refinement in blood sample preparation, CMS can be easily adapted to safety pharmacological studies [ 159 ]. CMS was used to determine NSAIDs (e.g., diclofenac, celecoxib, and tenoxicam) in Swiss albino mice [ 160 ].…”

Section: Sample Preparation Techniques In Bioanalyticsmentioning

confidence: 99%

Current developments of bioanalytical sample preparation techniques in pharmaceuticals

Ingle

Zeng

Jiang

et al. 2022

Journal of Pharmaceutical Analysis

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Section: Sample Preparation Techniques In Bioanalyticsmentioning

confidence: 99%

Current developments of bioanalytical sample preparation techniques in pharmaceuticals

Ingle

Zeng

Jiang

et al. 2022

Journal of Pharmaceutical Analysis

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“…Therefore, we then focused on assessing the effects of microsampling on toxicity evaluation under conditions after the administration of toxic drugs. To the best of our knowledge, previous reports investigating such an influence are limited to studies evaluating the influence on neurobehavioral clinical observation, respiratory parameters and some parameters in blood chemistry as well as the influence of a hematotoxic drug phenacetin or immunotoxic drug azathioprine (Li et al, 2020;Prior et al, 2015;Ohtsuka et al, 2022;Tochitani et al, 2022). In particular, we exclusively targeted the liver, an organ to which drugs pass first after absorption at the digestive tract.…”

Section: Introductionmentioning

confidence: 99%

Effect of microsampling (50 μL) on toxicological evaluation of methapyrilene, a hepatotoxic substance, in a collaborative 28-day study in female rats

Hattori

Yokoyama²,

Takumi

et al. 2023

J. Toxicol. Sci.

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Although microsampling of blood is recommended to promote the 3Rs in toxicokinetic (TK) evaluation, there are few reports applying microsampling in actual toxicity evaluation. Here, we assessed the effects of microsampling on toxicological evaluation of methapyrilene hydrochloride, a hepatotoxic substance. Female SD rats received methapyrilene hydrochloride orally at dose levels of 0 (vehicle), 10, and 30 mg/kg BW, once daily for 4 weeks. Each dose level included a microsampling group and a non-microsampling group (n = 5). In the microsampling groups, blood sampling (50 µL/time point) was performed at 6 time points on day 1 of administration and 7 time points on day 27-28; all the animals underwent necropsy on day 29. Toxicity studies and TK analysis were performed, and through these studies in 2 organizations, cross-organization validation of the effect on toxicity evaluation was conducted. In one organization, microsampling obscured changes in some parameters in hematology due to the administration of methapyrilene hydrochloride. In the other organization, although the relationship between the developing pattern of histopathological findings in the liver and the blood sampling was suspected, it was associated with poor reproducibility; this was considered as a change within a variation range of biological reactions. Each of these phenomena was observed in only one organization without consistency. In both organizations, no effect of blood microsampling was observed in other endpoints. In conclusion, microsampling is considered to be a technique applicable to safety studies of drugs showing hepatotoxicity, as it did not show a marked influence on the toxicological evaluation of methapyrilene hydrochloride.

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Non-toxic doses of modified titanium dioxide nanoparticles (m-TiO2NPs) in albino CFW mice

Basante-Romo

Gutiérrez-M

Camargo-Amado

2021

Heliyon

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Modified titanium dioxide (m-TiO 2 NPs) is a novel photocatalytic nanomaterial. Its level of toxicity was evaluated to be used in photodynamic treatment for cervical cancer. In the toxicity studies (Irwin test, acute and repeated doses (10 days)), female albino Swiss Webster (CFW) mice, 28 days old were used; the m-TiO 2 NPs was administered in single 300, 600 and 5,000 mg/kg of body weight (b.w) doses injected in the peritoneal zone. No adverse events or mortality were produced. Daily intraperitoneal doses of 300 and 600 mg/kg b.w every 24 h for 10 days did not produce adverse effects or mortality. There were no abnormal clinical signs or behavioral changes (neurological or physiological) in any of the mice. All organs exhibited normal architecture, and histological studies determined that m-TiO 2 NPs does not produce changes in the cells or tissues. Based on the test results, it is concluded that the m-TiO 2 NPs has not a toxic effect in doses equal to or less than 5,000 mg/kg b.w.

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Incorporation of capillary microsampling into whole body plethysmography and modified Irwin safety pharmacology studies in rats

Cited by 7 publications

References 24 publications

Current developments of bioanalytical sample preparation techniques in pharmaceuticals

Current developments of bioanalytical sample preparation techniques in pharmaceuticals

Effect of microsampling (50 μL) on toxicological evaluation of methapyrilene, a hepatotoxic substance, in a collaborative 28-day study in female rats

Non-toxic doses of modified titanium dioxide nanoparticles (m-TiO2NPs) in albino CFW mice

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