Incorporation of Achyrocline satureioides (Lam.) DC extracts into topical nanoemulsions obtained by means of spontaneous emulsification procedure

Bidone, Juliana; Zorzi, Giovanni K.; Carvalho, Edison Luis Santana; Simões, C. M. O.; Koester, Letícia Scherer; Bassani, Valquíria Linck; Teixeira, Hélder Ferreira

doi:10.1016/j.indcrop.2014.08.042

Cited by 23 publications

(33 citation statements)

References 34 publications

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“…However, such difference can be attributed to the 10-times higher concentration of quercetin (1 mg/mL) used by these authors. The results found for the NEE are in line with those reported by Bidone et al (2014) who studied nanoemulsions with similar extracts for 3-Omethylquercetin association (22). Concerning the high association efficiency found, this may be attributed to greater affinity of quercetin to the nanoemulsion internal phase (37).…”

Section: Discussion Preparation and Characterization Of Nanoemulsionssupporting

confidence: 90%

“…The physicochemical properties of the nanoemulsions are in accordance to formulations with similar composition obtained by spontaneous emulsification (18,22,32). However, some properties presented by the NEE were different than those for NEB and NEQ.…”

Section: Discussion Preparation and Characterization Of Nanoemulsionssupporting

confidence: 73%

“…For example, herpes infection by HSV-1 is known to damage both the skin and oral mucosa. The antiviral activity of A. satureioides ethanolic extract was already described (47), and recently, a nanoemulsion associating this extract was proposed for topical treatment of herpes (22).…”

Section: In Vitro Percutaneous Permeation Of Quercetinmentioning

confidence: 90%

“…They present kinetic stability in comparison to classic emulsions and offer higher surface area, optimal for topical applications (20,21). A welldocumented literature has shown that the association of isolated bioactive compounds into nanotechnology-based delivery systems may present several advantages when compared to conventional dosage forms, including increase in the solubility, reduction of side effects, controlled released of the active compound, and increase in the drug stability (22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant Effect of Nanoemulsions Containing Extract of Achyrocline satureioides (Lam) D.C.—Asteraceae

et al. 2015

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Abstract. Ethanolic extracts of Achyrocline satureioides have pronounced antioxidant activity mainly due to the presence of the flavonoid quercetin. However, direct topical application of the extract is not possible due to the presence of high amounts of ethanol. In this sense, nanoemulsions arise as an alternative for topical formulation associating molecules with limited aqueous solubility. This article describes the development of topical nanoemulsions containing either A. satureioides extract or one of its most abundant flavonoid, quercetin. Nanoemulsions composed of octyldodecanol, egg lecithin, water and extract (NEE), or quercetin (NEQ) were prepared by spontaneous emulsification. This process led to monodisperse nanoemulsions presenting a mean droplet size of approximately 200-300 nm, negative zeta potential, and high association efficiency. A study of quercetin skin retention using porcine skin which was performed using a Franz diffusion cell revealed a higher accumulation of quercetin in skin for NEE when compared to NEQ. Finally, the antioxidant activity of formulations was measured by thiobarbituric acidreactive species and the APPH model. A lower lipoperoxidation for the extract in respect to quercetin solution was observed. However, no difference between NEQ and NEE lipoperoxidation could be seen. The protection against lipoperoxidation by the formulations was also measured in the skin, where lower formation of reactive species was observed after treatment with NEE. In conclusion, this study shows the formulation effect on the physicochemical properties of nanoemulsions as well as on the skin retention and antioxidant activity of quercetin.

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Section: Discussion Preparation and Characterization Of Nanoemulsionssupporting

confidence: 90%

Section: Discussion Preparation and Characterization Of Nanoemulsionssupporting

confidence: 73%

Section: In Vitro Percutaneous Permeation Of Quercetinmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

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Antioxidant Effect of Nanoemulsions Containing Extract of Achyrocline satureioides (Lam) D.C.—Asteraceae

et al. 2015

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“…[15][16][17] Considering the advantages of nanoemulsions, many studies on incorporation of herbal drugs into these systems have been carried. 11,[18][19][20][21] Rapanea ferruginea (Ruiz & Pav) Mez (synonymy Myrsine coriacea) is a tree popularly known in Brazil as capororoca. Its leaves and stem barks are prepared as tea and are recommended, in popular use, to treat diseases of the urinary tract, as well as itching, rashes, hives, eczema, rheumatism, and diseases of the liver.…”

Section: Dal Mas Et Almentioning

confidence: 99%

Nanoemulsion as a carrier to improve the topical anti-inflammatory activity of stem bark extract of <em>Rapanea ferruginea</em>

Mas

Zermiani

Thiesen

et al. 2016

IJN

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The aim of this study was to develop nanoemulsion containing soft extract of stem bark of Rapanea ferruginea to improve the topical delivery and anti-inflammatory activity. The extract of R. ferruginea stem bark was incorporated into the oily phase of the nanoemulsion by the method of phase inversion at low energy. The developed nanoemulsion had an average droplet size of 47.88±8.20 nm and a polydispersibility index of 0.228. Uniformity of size, spherical shape of droplet, and absence of clusters were confirmed by transmission electronic microscopy. The zeta potential was −34.7±1.15 mV. The nanoemulsion showed a moderate degree of skin irritation in the agarose overlay assay in vitro. The content of the extract markers, myrsinoic acids A and B, was 54.10±0.08 and 53.03 μg/g in the formulation, respectively. The formulation demonstrated pseudoplastic and thixotropic rheological behavior. In vitro release of chemical markers was controlled by diffusion mechanism. An extract-loaded nanoemulsion showed a topical anti-inflammatory activity in a croton oil-induced edema ear model, with a decrease in tumor necrosis factor release and myeloperoxidase activity. The nanoemulsion was 160% more efficient than the conventional cream containing 0.13% of the extract. The nanoemulsion showed suitable properties as a carrier for topical use of R. ferruginea extract and the approach for improving the topical anti-inflammatory activity.

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A stability‐indicating ultra‐fast liquid chromatography method for the assay of the main flavonoids of Achyrocline satureioides (Marcela) in porcine skin layers and nanoemulsions

Balestrin

Fachel

Koester

et al. 2020

Phytochemical Analysis

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Introduction: Achyrocline satureioides (marcela or macela) is a plant widely used in folk medicine in South America. Recently, there has been increasing interest for the development of skin care products containing A. satureoides extracts, due to its welldocumented antioxidant, antiherpetic, and wound healing properties. Objectives: The present study aimed to develop and validate a yet unexplored stability-indicating and robust ultra-fast liquid chromatography (UFLC) method for the simultaneous quantification of the main flavonoids of A. satureioides in extracts, nanoemulsions, and porcine skin layers. Material and methods: The chromatographic separation of flavonoids quercetin, luteolin, and 3-O-methylquercetin was performed on a Luna C18 analytical column (100 mm × 2.0 mm i.d.; particle size 2.5 μm) using isocratic elution with methanol/phosphoric acid 1% (48:52 v/v) with a flow rate of 0.3 mL/min at 40 C. Results: The method was found to be specific, linear (R > 0.998), precise, accurate, and robust for all flavonoids assayed in A. satureioides extract, nanoemulsions, and porcine ear skin. A low matrix effect was noted for all complex matrices. The stability-indicating UFLC method was evaluated by submitting isolated flavonoids, a mixture of standards, and A. satureioides extract to acidic, alkaline, oxidative, UV-A/UV-C light, and thermal stress conditions. No peaks were found co-eluting with the flavonoids of interest in all matrices. The robustness of the method was confirmed using Plackett-Burman experimental design. Conclusion: The short run time (8 min) and reliability of the method could be useful for the determination of A. satureioides flavonoids in topical product development since extracts of this medicinal plant have been used to treat various skin disorders.

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Incorporation of Achyrocline satureioides (Lam.) DC extracts into topical nanoemulsions obtained by means of spontaneous emulsification procedure

Cited by 23 publications

References 34 publications

Antioxidant Effect of Nanoemulsions Containing Extract of Achyrocline satureioides (Lam) D.C.—Asteraceae

Antioxidant Effect of Nanoemulsions Containing Extract of Achyrocline satureioides (Lam) D.C.—Asteraceae

Nanoemulsion as a carrier to improve the topical anti-inflammatory activity of stem bark extract of <em>Rapanea ferruginea</em>

A stability‐indicating ultra‐fast liquid chromatography method for the assay of the main flavonoids of Achyrocline satureioides (Marcela) in porcine skin layers and nanoemulsions

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