Incorporation and controlled release of a hydrophilic antibiotic using poly(lactide-co-glycolide)-based electrospun nanofibrous scaffolds

Kim, Kwangsok; Luu, Yen K.; Chang, Ching‐Wen; Fang, Dufei; Hsiao, Benjamin S.; Chu, Benjamin; Hadjiargyrou, Michael

doi:10.1016/j.jconrel.2004.04.009

Cited by 702 publications

(445 citation statements)

References 21 publications

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“…So a high initial release rate in the first few days after the implant is best for eliminating the intruding bacteria that are introduced during the implantation and systemically afterwards before they begin to proliferate. For the few bacteria that may survive the initial burst, a continued drug release is necessary to prevent their further population and inhibit the occurrence of latent infection [38]. So this 3-week prolonged drug release is considered to be an optimal treatment.…”

Section: Discussionmentioning

confidence: 99%

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

Xue

Liu³

et al. 2014

Biomaterials

321

217

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Email address: sharell@126.com (R. Shi). AbstractInfection is the major reason for guided tissue regeneration/guided bone regeneration (GTR/GBR) membrane failure in clinical application. In this work, we developed GTR/GBR membranes with localized drug delivery function to prevent infection by electrospinning of poly(ε-caprolactone) (PCL) and gelatin blended with metronidazole (MNA). Acetic acid (HAc) was introduced to improve the miscibility of PCL and gelatin to fabricate homogeneous hybrid nanofiber membranes. The effects of the addition of HAc and the MNA content (0,1,5,10,20,30, and 40 wt.% of polymer) on the properties of the membranes were investigated. The membranes showed good mechanical properties, appropriate biodegradation rate and barrier function. The controlled and sustained release of MNA from the membranes significantly prevented the colonization of anaerobic bacteria. Cells could adhere to and proliferate on the membranes without cytotoxicity until the MNA content reached 30%.Subcutaneous implantation in rabbits for 8 months demonstrated that MNA-loaded membranes evoked a less severe inflammatory response depending on the dose of MNA than bare membranes. The biodegradation time of the membranes was appropriate for tissue regeneration. These results indicated the potential for using MNA-loaded PCL/gelatin electrospun membranes as anti-infective GTR/GBR membranes to optimize clinical application of GTR/GBR strategies.

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Section: Discussionmentioning

confidence: 99%

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

Xue

Liu³

et al. 2014

Biomaterials

321

217

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“…Blending the bioactive factor into the polymer solution during electrospinning has been used to load proteins, 15,40,43,73 small molecules, 15,74 and nucleic acids [75][76][77] for release. Depending on the relative properties of the polymer and bioactive factors, this method can lead to the factors being homogenously dispersed in the fibers, 15 randomly dispersed in aggregates throughout the fibers, 15,73 or localized to the surface of the fibers.…”

Section: Bioactive Factor Loadingmentioning

confidence: 99%

Polymeric Nanofibers in Tissue Engineering

Dahlin

Kasper

Mikos

2011

Tissue Engineering Part B: Reviews

287

193

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Polymeric nanofibers can be produced using methods such as electrospinning, phase separation, and selfassembly, and the fiber composition, diameter, alignment, degradation, and mechanical properties can be tailored to the intended application. Nanofibers possess unique advantages for tissue engineering. The small diameter closely matches that of extracellular matrix fibers, and the relatively large surface area is beneficial for cell attachment and bioactive factor loading. This review will update the reader on the aspects of nanofiber fabrication and characterization important to tissue engineering, including control of porous structure, cell infiltration, and fiber degradation. Bioactive factor loading will be discussed with specific relevance to tissue engineering. Finally, applications of polymeric nanofibers in the fields of bone, cartilage, ligament and tendon, cardiovascular, and neural tissue engineering will be reviewed.

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“…Mostly PGA and PLA non-woven scaffolds were used in TE research [89][90][91]. These fibrous structures have high porosity (up to 95%) with interconnected pores and high surface area to volume ratio, though fibre diameter is confined in the range 10-15 µm.…”

Section: Textile Technologiesmentioning

confidence: 99%

“…This opportunity has been extensively exploited to load fibres with biomolecules that were intended to be released upon contact with the biological environment. Among the several drugs employed, antibiotics [87][88][89], anti-inflammatory drugs [90][91][92] and anticancer drugs [93] have been mainly investigated.…”

Section: "Composite" Electrospun Scaffoldsmentioning

confidence: 99%

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Gualandi

2011

Springer Theses

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and some lactide copolymers) and of non-commercial polyesters (i.e. poly-ω-pentadecalactone and some of its copolymers) were explored and discussed.Two techniques were employed to fabricate scaffolds: supercritical carbon dioxide (scCO 2 ) foaming and electrospinning (ES). The former is a powerful technology that enables to produce 3D microporous foams by avoiding the use of solvents that can be toxic to mammalian cells. The scCO 2 process, which is II commonly applied to amorphous polymers, was successfully modified to foam a highly crystalline poly(ω-pentadecalactone-co-ε-caprolactone) copolymer and the effect of process parameters on scaffold morphology and thermo-mechanical properties was investigated.In the course of the present research activity, sub-micrometric fibrous nonwoven meshes were produced using ES technology. Electrospun materials are considered highly promising scaffolds because they resemble the 3D organization of native extra cellular matrix. A careful control of process parameters allowed to fabricate defect-free fibres with diameters ranging from hundreds of nanometers to several microns, having either smooth or porous surface. Moreover, versatility of ES technology enabled to produce electrospun scaffolds from different polyesters as well as "composite" non-woven meshes by concomitantly electrospinning different fibres in terms of both fibre morphology and polymer material. The 3D-architecture of the electrospun scaffolds fabricated in this research was controlled in terms of mutual fibre orientation by properly modifying the instrumental apparatus. This aspect is particularly interesting since the micro/nano-architecture of the scaffold is known to affect cell behaviour.Since last generation scaffolds are expected to induce specific cell response, the present research activity also explored the possibility to produce electrospun scaffolds bioactive towards cells. Bio-functionalized substrates were obtained by loading polymer fibres with growth factors (i.e. biomolecules that elicit specific cell behaviour) and it was demonstrated that, despite the high voltages applied during electrospinning, the growth factor retains its biological activity once

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Incorporation and controlled release of a hydrophilic antibiotic using poly(lactide-co-glycolide)-based electrospun nanofibrous scaffolds

Cited by 702 publications

References 21 publications

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

Polymeric Nanofibers in Tissue Engineering

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

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