Incomplete viral genome in a non‐virogenic mouse tumour cell line (RVP<sub>3</sub>) transformed by prague strain of avian sarcoma virus

Svoboda, Jan; Popovic, M; Sainerová, H; Mach, O; Shoyab, Mohammed; Baluda, M A

doi:10.1002/ijc.2910190617

Cited by 22 publications

(14 citation statements)

References 21 publications

(11 reference statements)

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“…The results show that the integration of HBV-specific DNA sequences into the liver cells may not only occur as late and rare events as in the case of primary liver carcinomas, but also rather early and systemically as in another HBV-related liver disease, namely cirrhosis. As has been shown in various other virus-host systems (Gallimore et al, 1974;Svoboda et al, 1977), integration of only a part of the virus genome can take place. Apparently, malignant transformation can result from transfection of specific subgenomic fragments of virus DNA or cDNA (Canaani et al, 1979;Anderson et al, 1979;Copeland et al, 1980;Graham et al, 1974).…”

Section: Discussionmentioning

confidence: 69%

Detection of Hepatitis B Virus-specific DNA in the Genomes of Human Hepatocellular Carcinoma and Liver Cirrhosis Tissues

Koshy

Maupas

Müller³

et al. 1981

Journal of General Virology

102

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Section: Discussionmentioning

confidence: 69%

Detection of Hepatitis B Virus-specific DNA in the Genomes of Human Hepatocellular Carcinoma and Liver Cirrhosis Tissues

Koshy

Maupas

Müller³

et al. 1981

Journal of General Virology

102

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“…Svoboda et al (24) obtained evidence consistent with integration of only a partial provirus in a mammalian cell line nonproductively transformed with Rous sarcoma virus because they observed lower plateau hybridization values for these cells than with a producer cell line. However, other possibilitiese.g., only a small population of the cells being infected with whole provirus-are alternative interpretations of these results.…”

Section: Methodsmentioning

confidence: 98%

Retrovirus sequences in a leukemic gibbon and its contact: evidence for partial provirus in the nonleukemic gibbon.

Wong‐Staal¹,

Reitz²,

Gallo³

1979

Proc. Natl. Acad. Sci. U.S.A.

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ABSTRACr Integrated viral DNA sequences were detected in tissues from two gibbon apes, a leukemic gibbon (60-1) from whose leukocytes a distinct strain of gibbon ape leukemia virus (GaLVH) was isolated, and gibbon 604, a contact of 6G-1 from the same colony that had uremia and cachexia of unknown origin. Although 6G4 had no detectable neoplasia or viral proteins, its serum contained persistent antibody against GaLV antigens. Whereas DNA from most of the tissues of 60-1 contained GaLV provirus, DNA from only three tissues (kidney, spleen, and liver) from 604 showed detectable viral sequences, and the extent of hybridization in each case was lower than with 6G-1. After cleavage with BamHI, two virus-specific DNA fragments were detected in tissues of 60-1. Only one of these fragments was detected in the positive tissues of 6G4. The results indicate that: (i) 6G4 was exposed to and infected by GaLV; (ii) early target sites for infection of gibbon by GaLV may be limited to a few tissues; and (iii) infection can be contained by integration of only partial provirus in a few tissues.We recently reported the isolation of a type C virus from a gibbon with spontaneous malignant lymphoma and leukemia (1). This virus, designated GaLVH, is in the same family that contains other gibbon ape leukemia virus isolates (2-5) and the simian sarcoma virus (SSV) and simian sarcoma-associated virus (SSAV) isolated from a fibrosarcoma of a pet woolly monkey (6, 7). Although closely related, the members of this group including GaLVH may be distinguished from each other by analysis of their RNA (8) or of the gp70 envelope protein (9). Proviral DNA and viral RNA and proteins were detected in most tissues of the leukemic gibbon (6G-1), and virus was recovered from all tissues, including tissues of the oral cavity (1). In addition to leukemic gibbon 6G-1, at least four of the eight animals of this colony were also exposed to GaLVH as indicated by the detection of serum antibodies (9, 10) even though none was viremic. For unknown reasons, one of the antibody-positive, nonleukemic, nonviremic animals, designated 6G-4, developed renal failure, became emaciated, and died. This provided an opportunity for search for proviral sequences in DNA from various tissues of this gibbon and to compare these sequences to those of 6G-1, the leukemic, viremic animal.Here we report results of molecular hybridization experiments showing that proviral sequences were present in 6G-4 but only in DNA extracted from liver, kidney, and spleen, and the plateau hybridization values were much lower than those obtained with DNA from 6G-1. Using restriction enzyme cleavage of cellular DNA followed by hybridization of the fragments to 125I-labeled viral RNA, we confirmed and extended our previous finding of proviral DNA in tissues from 6G-1. Two viral fragments were detected after cleavage with BamHI. More interestingly, DNA from the positive tissues of 6G-4 was shown to contain only one of these viral fragments, indicating that only part of the GaLV provirus is integrated ...

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“…First, we studied one such mouse cell line from which no virus was rescuable-even after helper virus complementation-but which retained a specific transplantation antigen (TSTA) characteristic of RSV tumors of different species origin (31). We found, in collaboration with Marcel Baluda, that it carried about one third of the RSV genome (32). Despite the encouraging start, we revealed that the mouse tumor cell line used underwent karyological changes such as anomalous metacentric chromosomes and showed fluctuations in hybridization experiments.…”

Section: The Src Oncogenementioning

confidence: 94%

On board a raft or boat in the retrovirus sea

Svoboda¹

2016

Proc. Natl. Acad. Sci. U.S.A.

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This article summarizes the essential steps in understanding the chicken Rous sarcoma virus (RSV) genome association with a nonpermissive rodent host cell genome. This insight was made possible by in-depth study of RSV-transformed rat XC cells, which were called virogenic because they indefinitely carry virus genetic information in the absence of any infectious virus production. However, the virus was rescued by association of XC cells with chicken fibroblasts, allowing cell fusion between both partners. This and additional studies led to the interpretation that the RSV genome gets integrated into the host cell genome as a provirus. Study of additional rodent virogenic cell lines provided evidence that the transcript of oncogene v-src can be transmitted to other retroviruses and produce cell transformation by itself. As discussed in the text, two main questions related to nonpermissiveness to retrovirus infection remain to be solved. The first is changes in the retrovirus envelope gene allowing virus entry into a nonpermissive cell. The second is the nature of the permissive cell functions required by the nonpermissive cell to ensure infectious virus production. Both lines of investigation are being pursued.

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Incomplete viral genome in a non‐virogenic mouse tumour cell line (RVP₃) transformed by prague strain of avian sarcoma virus

Cited by 22 publications

References 21 publications

Detection of Hepatitis B Virus-specific DNA in the Genomes of Human Hepatocellular Carcinoma and Liver Cirrhosis Tissues

Detection of Hepatitis B Virus-specific DNA in the Genomes of Human Hepatocellular Carcinoma and Liver Cirrhosis Tissues

Retrovirus sequences in a leukemic gibbon and its contact: evidence for partial provirus in the nonleukemic gibbon.

On board a raft or boat in the retrovirus sea

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Incomplete viral genome in a non‐virogenic mouse tumour cell line (RVP3) transformed by prague strain of avian sarcoma virus

Cited by 22 publications

References 21 publications

Detection of Hepatitis B Virus-specific DNA in the Genomes of Human Hepatocellular Carcinoma and Liver Cirrhosis Tissues

Detection of Hepatitis B Virus-specific DNA in the Genomes of Human Hepatocellular Carcinoma and Liver Cirrhosis Tissues

Retrovirus sequences in a leukemic gibbon and its contact: evidence for partial provirus in the nonleukemic gibbon.

On board a raft or boat in the retrovirus sea

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Incomplete viral genome in a non‐virogenic mouse tumour cell line (RVP₃) transformed by prague strain of avian sarcoma virus