Incomplete cellular depopulation may explain the high failure rate of bovine ureteric grafts

Spark, J.I.; Yeluri, Sashidhar; Derham, Chris; Wong, Yew Toh; Leitch, Derek

doi:10.1002/bjs.6052

Cited by 54 publications

(31 citation statements)

References 17 publications

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“…Despite these obvious advantages, tissue engineering approaches using decellularized material are often limited due to batch-to-batch variations of the accessible allograft raw material [12]. Furthermore, the less favorable use of xenografts may elicit significant immune response in the recipient due to differences in the primary structure of the residual proteins in the ECM [13,14]. We hypothesize that the vasculature of the human placenta, in particular the placental chorionic plate, has great potential as a material source for human vascular allografts for a wide range of inner diameter sizes.…”

Section: Introductionmentioning

confidence: 98%

Decellularized human placenta chorion matrix as a favorable source of small-diameter vascular grafts

et al. 2016

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Section: Introductionmentioning

confidence: 98%

Decellularized human placenta chorion matrix as a favorable source of small-diameter vascular grafts

et al. 2016

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“…[63, 64] Strategies to stabilize ECM-based vascular grafts to prevent dilation include chemical crosslinking using carbodiimide chemistry to reduce ECM degradation[63] and perivascular polymer coating with electrospun PCL to prevent expansion. [65] However, stabilization of the ECM scaffold with either chemical crosslinking or polymer coating could also lead to increased stiffness and compliance mismatch between native arteries and vascular grafts and result in a severe inflammatory response and increased neointimal hyperplasia.…”

Section: Discussionmentioning

confidence: 99%

Vascular scaffolds with enhanced antioxidant activity inhibit graft calcification

et al. 2017

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There is a need for off-the-shelf, small-diameter vascular grafts that are safe and exhibit high long-term patency. Decellularized tissues can potentially be used as vascular grafts; however, thrombogenic and unpredictable remodeling properties such as intimal hyperplasia and calcification are concerns that hinder their clinical use. The objective of this study was to investigate the long-term function and remodeling of extracellular matrix (ECM)-based vascular grafts composited with antioxidant poly(1, 8-octamethylenecitrate-co-cysteine) (POCC) with or without immobilized heparin. Rat aortas were decellularized to create the following vascular grafts: 1) ECM hybridized with POCC (Poly-ECM), 2) Poly-ECM subsequently functionalized with heparin (Poly-ECM-Hep), and 3) non-modified vascular ECM. Grafts were evaluated as interposition grafts in the abdominal aorta of adult rats at three months. All grafts displayed antioxidant activity, were patent, and exhibited minimal intramural cell infiltration with varying degrees of calcification. Areas of calcification co-localized with osteochondrogenic differentiation of vascular smooth muscle cells, lipid peroxidation, oxidized DNA damage, and cell apoptosis, suggesting an important role for oxidative stress in the calcification of grafts. The extent of calcification within grafts was inversely proportional to their antioxidant activity: Poly-ECM-Hep>ECM>Poly-ECM. The incorporation of antioxidants into vascular grafts may be a viable strategy to inhibit degenerative changes.

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“…Beside the potential risk of viral transmission from animal tissue, the implantation of decellularized xenografts gave disappointing results. Indeed, the treatment of patients with decellularized bovine ureters resulted in high failure rate probably due to the presence of residual immunogenic contaminants, such as galactose-alpha-1,3-galactose (alpha gal) [17]. Thus, the use of homologous AMs seems to be a more suitable approach for vascular replacement.…”

Section: Introductionmentioning

confidence: 99%

Blood Vessel-Derived Acellular Matrix for Vascular Graft Application

Dall’Olmo

Zanusso

Liddo

et al. 2014

BioMed Research International

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To overcome the issues connected to the use of autologous vascular grafts and artificial materials for reconstruction of small diameter (<6 mm) blood vessels, this study aimed to develop acellular matrix- (AM-) based vascular grafts. Rat iliac arteries were decellularized by a detergent-enzymatic treatment, whereas endothelial cells (ECs) were obtained through enzymatic digestion of rat skin followed by immunomagnetic separation of CD31-positive cells. Sixteen female Lewis rats (8 weeks old) received only AM or previously in vitro reendothelialized AM as abdominal aorta interposition grafts (about 1 cm). The detergent-enzymatic treatment completely removed the cellular part of vessels and both MHC class I and class II antigens. One month after surgery, the luminal surface of implanted AMs was partially covered by ECs and several platelets adhered in the areas lacking cell coverage. Intimal hyperplasia, already detected after 1 month, increased at 3 months. On the contrary, all grafts composed by AM and ECs were completely covered at 1 month and their structure was similar to that of native vessels at 3 months. Taken together, our findings show that prostheses composed of AM preseeded with ECs could be a promising approach for the replacement of blood vessels.

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Incomplete cellular depopulation may explain the high failure rate of bovine ureteric grafts

Abstract: Graft failure with aneurysmal dilatation and thrombosis in complex arteriovenous conduits using bovine ureter may be due to residual xenoantigens.

Cited by 54 publications

References 17 publications

Decellularized human placenta chorion matrix as a favorable source of small-diameter vascular grafts

Decellularized human placenta chorion matrix as a favorable source of small-diameter vascular grafts

Vascular scaffolds with enhanced antioxidant activity inhibit graft calcification

Blood Vessel-Derived Acellular Matrix for Vascular Graft Application

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