Decellularized human placenta chorion matrix as a favorable source of small-diameter vascular grafts

Schneider, Karl H.; Aigner, Petra; Holnthoner, Wolfgang; Monforte, Xavier; Nürnberger, Sylvia; Rünzler, Dominik; Redl, Heinz; Teuschl, Andreas

doi:10.1016/j.actbio.2015.09.038

Cited by 87 publications

(94 citation statements)

References 58 publications

(58 reference statements)

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“…However, there is evidence that excessive residual SDS in ECM can be cytotoxic and inhibit cell infiltration. [32][33][34] Thus, it is necessary to ensure that the decellularized scaffold is noncytotoxic after washing. The CCK-8 test measured cell proliferation and survival in D-ovary leaching solution compared with that in the standard medium.…”

Section: Discussionmentioning

confidence: 99%

Xenogeneic Decellularized Scaffold: A Novel Platform for Ovary Regeneration

Liu

Lin

Zhuo

et al. 2017

Tissue Engineering Part C: Methods

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Women younger than 40 years may face early menopause because of premature ovarian failure (POF). The cause of POF can be idiopathic or iatrogenic, especially the cancer-induced oophorectomy and chemo-or radiation therapy. The current treatments, including hormone replacement therapy (HRT) and cryopreservation techniques, have increased risk of ovarian cancer and may reintroduce malignant cells after autografting. Decellularization technique has been regarded as a novel regenerative medicine strategy for organ replacement, wherein the living cells of an organ are removed, leaving the extracellular matrix (ECM) for cellular seeding. This study aimed to produce a xenogeneic decellularized ovary (D-ovary) scaffold as a platform for ovary regeneration and transplantation. We have developed a novel decellularization protocol for porcine ovary by treatment with physical, chemical, and enzymatic methods. Using hematoxylin and eosin (H&E) staining, DAPI staining, scanning electron microscopy (SEM), and quantitative analysis, this approach proved effective in removing cellular components and preserving ECM. Furthermore, the results of biological safety evaluation demonstrated that the D-ovary tissues were noncytotoxic for rat ovarian cells in vitro and caused only a minimal immunogenic response in vivo. In addition, the D-ovary tissues successfully supported rat granulosa cell penetration ex vivo and showed an improvement in estradiol (E2) hormone secretion.

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Section: Discussionmentioning

confidence: 99%

Xenogeneic Decellularized Scaffold: A Novel Platform for Ovary Regeneration

Liu

Lin

Zhuo

et al. 2017

Tissue Engineering Part C: Methods

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“…Common physical treatments involve sonication, pressure, shear forces, freezing and thawing 73 . Hypotonic and hypertonic solutions have also been found to be effective at mediating the cell lysing process 71 . Azhim and coworkers have performed studies on decellularization of porcine aorta via sonication at the luminal side while immersed in 2% SDS (sodium dodecyl sulfate) solution 74 .…”

Section: Decellularization Strategiesmentioning

confidence: 99%

“…The most popular zwitterionic detergent that has been employed to decellularize arteries is 3-[(3 cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS). Both Triton X-100 and CHAPS elute the glycosaminoglycans present in biological tissues 71 . Finally, EDTA and EGTA have also been included in decellularization protocols to facilitate cell removal by chelating calcium and magnesium and disrupt cell-cell and cell-matrix adhesion.…”

Section: Decellularization Strategiesmentioning

confidence: 99%

“…Another approach utilized a perfusion-driven process that could successfully decellularize tissues using low concentrations of chemicals and short exposure times 71 . Vessels harvested from the placenta were attached to a perfusion capillary and assembled to a recirculation system with a peristaltic pump, which allowed for application of reverse osmotic pressure for cell lysis.…”

Section: Decellularization Strategiesmentioning

confidence: 99%

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Cell-free vascular grafts: Recent developments and clinical potential

Row

Santandreu

Swartz³

et al. 2017

Technology

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Recent advances in vascular tissue engineering have led to the development of cell-free grafts that are available off-the-shelf for on demand surgery. Challenges associated with cell-based technologies including cell sourcing, cell expansion and long-term bioreactor culture motivated the development of completely cell-free vascular grafts. These are based on decellularized arteries, decellularized cultured cell-based tissue engineered grafts or biomaterials functionalized with biological signals that promote in situ tissue regeneration. Clinical trials undertaken to demonstrate the applicability of these grafts are also discussed. This comprehensive review summarizes recent developments in vascular graft technologies, with potential applications in coronary artery bypass procedures, lower extremity bypass, vascular injury and trauma, congenital heart diseases and dialysis access shunts, to name a few.

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“…Scaffolds of decellularized human placenta have been used in tissue engineering for skin wound healing [Choi et al, 2013], vascular grafts with a small diameter [Schneider et al, 2016], adipose tissue regeneration [Flynn et al, 2006], liver diseases [Jung et al, 2011], and cartilage degradation . Recently, Barreto et al [2018] characterized bovine placentas as a tool for use as a biological scaffold, with possibilities of recellularization due to preservation of vascular structures in the decellularization process.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Canine Placenta Decellularization: An Alternative Source of Biological Scaffolds for Regenerative Medicine

Matias

Rigoglio

Carreira

et al. 2018

Cells Tissues Organs

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Due to the scarcity of tissues and organs for transplantation, the demand for bioengineered tissues is increasing with the advancement of technologies and new treatments in human and animal regenerative medicine. Thus, decellularized placental extracellular matrix (ECM) has emerged as a new tool for the production of biological scaffolds for subsequent recellularization and implantation for recovery of injured areas or even for replacement of organ and tissue fractions. To be classified as an ideal biological scaffold, the ECM must be acellular and preserve its proteins and physical features to be useful for cellular adhesion. In this context, we developed a process of decellularization of canine placentas with 35 and 40 days of gestation using dodecyl sulfate sodium under immersion and agitation in sterile conditions. Before use of this scaffold in recellularization processes, the decellularization efficiency needs to be confirmed by the absence of cellular content and an irrelevant amount of reminiscent DNA. Both vasculature architecture and ECM proteins, such as collagen types I, III, and IV, laminin, and fibronectin, were preserved with our method. In this way, we established a new biological scaffold model that could be used for recellularization in regenerative medicine of tissues.

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Decellularized human placenta chorion matrix as a favorable source of small-diameter vascular grafts

Cited by 87 publications

References 58 publications

Xenogeneic Decellularized Scaffold: A Novel Platform for Ovary Regeneration

Xenogeneic Decellularized Scaffold: A Novel Platform for Ovary Regeneration

Cell-free vascular grafts: Recent developments and clinical potential

Optimization of Canine Placenta Decellularization: An Alternative Source of Biological Scaffolds for Regenerative Medicine

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