Incompatibility studies by high performance thin-layer chromatography: In case of curcumin

Abstract

C urcumin[1,7-bis (4-hydroxy-3-methoxy-phenyl) hepta-1, 6-diene-3, 5-dione] is one of the component present in the turmeric. Curcumin has been in use for its medicinal benefits since centuries and its therapeutic potential is continuously explored through various researchers throughout the world. To investigate the interaction of curcumin with commonly used excipients such as microcrystalline cellulose, starch, colloidal silica, talc, ascorbic acid, lactose, ethyl cellulose (EC), sodium carboxymethylcellulose (Na-CMC), hydroxyl propyl methyl cellulose and magnesium stearate. High performance thin-layer chromatography (HPTLC) is commonly used technique for the determination of phytoconstituents, but its application in incompatibility studies is still not investigated. Thus, we initiated our study with HPTLC followed by Fourier transform infrared and differential scanning calorimetry. Since interaction of curcumin with ascorbic acid, EC, Na-CMC and Mg-stearate confirmed by all three techniques these four excipients should be avoided during the formulation development of curcumin. The presented study also establishes HPTLC's usefulness in such interaction studies.

One-step, ultrasonication-mobilized, solvent-free extraction/synthesis of nanocurcumin from turmeric

“…Curcumin contains two carbonyl groups showing bands at around 1640 cm À1 . 45 curcumin extracted using the WBS 1-5 and PUS 1-10 experimental sets. As observed in the gure, all the major peaks characteristic of curcumin were obtained from the sonicated samples.…”

Comparison of PrestoBlue® and plating method to evaluate antimicrobial activity of ascorbic acid, boric acid and curcumin in an in vitro gastrointestinal model

“…During the oxidation of AA to dehydroascorbic, hydrogen peroxide is produced as a by‐product, which negatively affects the phenolic compounds and therefore are produced phenoxy radicals in CUR, which are less reactive (Shrivastava et al . ; Priyadarsini ; Samad et al . ).…”

The electrochemical characterization of curcumin and its selective detection in Curcuma using a graphene-modified electrode

“…10,11 In the literature, some methods for the determination of curcumin are reported, such as high-performance liquid chromatography (HPLC), [12][13][14] spectrouorimetry, 15,16 and highperformance thin-layer chromatography. 17 Besides, UV-vis spectrophotometric and chemiluminescent dynamic methods 18 were developed for investigating the antioxidant activity of curcumin. Comparing these, the electroanalytical technique has the advantages of simplicity and high sensitivity, and simultaneously can give some information about the reaction mechanism between medical and biological molecules.…”