Inclusion of Glutathione Peroxidase on Polypyrrole Modified Electrode, to Be Assayed as Glutathione Sensor

Abstract

In this work it was obtained a modified electrode by inclusion of the enzyme glutathione peroxidase (GPx) on a conductive polymer, polypyrrole (PPy), deposited on a graphite electrode (GC|PPy,GPx) for the amperometric detection of reduced glutathione (GSH) in presence of hydrogen peroxide. Also, working conditions were optimized in order to prepare the electrode GC|PPy,GPx, which were tested for different concentrations of monomer and enzyme, electrochemical type of perturbance (by fixed potential or cyclic voltammetry) deposition potential and time (PPy film thickness, GPx), etc. by testing the response of the electrodes versus GSH in presence of hydrogen peroxide at physiological pH and temperature. At optimal experimental conditions, the achieved electrode gives a characteristic response for the analyte, which means that this modified electrode can be proposed as a selective GSH sensor, since it was shown that the enzyme maintains its activity during experimental manipulations and responds in a highly reproducible mode, so its use is proposed as an amperometric biosensor which is highly selective and stable.

A PRELIMINARY STUDY ON ELECTROCATALYTIC REDUCTION OF CO2 USING FAC-ReI(CO)3(4,4′-DI-METHYL-2,2′-BIPYRIDYL)((E)-2-((3-AMINO-PYRIDIN-4-YLIMINO)-METHYL)-4,6-DI-TERT-BUTYLPHENOL))+ COMPLEX

“…This work is framed in the development of modulating the photophysical properties on the rhenium(I) tricarbonyl core with these kinds of ancillary ligands. [60][61] From the chemical point of view, the modified physicochemical and electrochemical properties modulated the ancillary ligand aimed to obtain catalytic applications of the new rhenium (I) complexes in the CO 2 reduction. [62][63] Supporting Information: Supplementary data associated with this article can be found, in the online version.…”

“…The Epc value for the reversible couple that was observed at -1.38 V vs SCE under inert atmosphere is now shifted to -1.30 V vs SCE (80 mV less negative) and the following reduction process is now an intense unsolved current discharge. [59][60]…”

DESIGN AND EVALUATION OF A Hg(II) SENSOR BASED ON THE RESPONSE OF A POLY(3,4-ETHYLENEDIOXYTHIOPHENE) MODIFIED ELECTRODE

“…sensors, batteries, fuel cells, photocells, light emitting diodes, etc. makes these materials one of the most studied topics at present [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] . Among these polymers polyaniline, polypyrrole, polythiophene and its derivatives stand out.…”