Inclusion of different exogenous fibrolytic enzymes to dry jowar fodder and their effect on in vitro total gas production

Abstract

Aim: Our objective was to estimate in-vitro gas production from dry jowar fodder added with different concentrations of exogenous fibrolytic enzymes (EFEs) like neutral cellulase and fungal xylanase. Materials and Methods:34 different samples of dry jowar fodder were prepared according to different concentrations of neutral cellulase, fungal xylanase and neutral cellulase + fungal xylanase (1:1). Sample not containing any enzymes was considered as the control group. These 34 samples were subjected to further in vitro gas production analysis.Results: Statistically, significantly higher (P<0.05) potential gas production was recorded for 0.7 % at 6 hr period, 0.7 % at 12 hr period, 0.7 %, 0.8 % at 18 hr period and 0.7 %, 0.8 % at 24 hr period in the samples treated with neutral cellulase. Significantly higher potential gas production was recorded for 0.5 %, 0.8 % at 6 hr period, 0.5 %, 0.6 %, 0.8 % at 12 hr period, 0.8 % at 18 hr period and 0.5 %, 0.6 %, 0.8 % at 24 hr period in the samples treated with fungal xylanase. Significantly higher potential gas production was recorded for 0.6 %, 0.6 %, 0.8 % at 6 hr period, 0.6 %, 0.8 % at 12 hr period, 0.6 %, 0.8 % at 18 hr period and 0.6 %, 0.8 % at 24 hr period in the samples treated with mixture of neutral cellulase + fungal xylanase (1:1). Conclusion:Addition of neutral cellulase and fungal xylanase into the samples of dry jowar fodder increased in vitro total potential gas production. EFEs increase substrate degradation and there by improve the nutritive value of dry jowar fodder.

In vitro evaluation of total mixed ration supplemented with exogenous fibrolytic enzymes for crossbred cows

“…Supplementation of higher levels (0.9% and 1%) of fibrolytic enzymes had no further beneficial effect on in vitro gas production [24]. Similarly, significant effect of EFE supplementation in roughage diet [18], maize stover and sugarcane bagasse [25], and sorghum straw [21] was observed for in vitro gas production.…”

“…The higher (p≤0.05) in vitro gas production observed as 96.33 ml and 96.00 ml when dry sorghum supplemented with EFE (1:1 mixture of neutral cellulase-3000 units/g and fungal xylanase-200000 units/g) at 0.6 and 0.8%, respectively, than dry sorghum supplemented without or with lower levels of fibrolytic enzymes (0, 0.01, 0.1, 0.2, 0.3, and 0.5%). Supplementation of higher levels (0.9% and 1%) of fibrolytic enzymes had no further beneficial effect on in vitro gas production [ 24 ]. Similarly, significant effect of EFE supplementation in roughage diet [ 18 ], maize stover and sugarcane bagasse [ 25 ], and sorghum straw [ 21 ] was observed for in vitro gas production.…”