Incidence of spontaneous abortion among pregnancies produced by assisted reproductive technology

Wang, Jim X.; Norman, Robert J.; Wilcox, Allen J.

doi:10.1093/humrep/deh078

Cited by 130 publications

(82 citation statements)

References 40 publications

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“…This assumption is corroborated by recent reports that show ART to be correlated with an increased frequency of congenital diseases associated with imprinting defects such as Beckwith-Wiedeman syndrome (BWS) and Angelman syndrome (AS) [1,3,9,24,25]. The increased frequency of miscarriages in ART could also be partly explained by these DNA methylation aberrations [5,15,46]. Considering the severity of the possible consequences to children born by ART, it is essential to assure that cryopreservation does not alter the DNA methylation patterns.…”

Section: Introductionmentioning

confidence: 72%

Routine cryopreservation of spermatozoa is safe — Evidence from the DNA methylation pattern of nine spermatozoa genes

Kläver¹,

Bleiziffer²,

Redmαnn³

et al. 2012

J Assist Reprod Genet

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Purpose Assess short-and mid-term impact of cryopreservation on DNA methylation status of different genes in spermatozoa. Methods Semen samples from 10 healthy normozoospermic men were collected at the Department of Clinical Andrology of the Centre of Reproductive Medicine and Andrology (Muenster, Germany). Each was divided into four equal aliquots: 1) untreated, 2) diluted in cryoprotectant, 3) short term (2 days) cryopreserved and 4) mid term (4 weeks) cryopreserved. Samples were "swim-up" purified prior to analysis. DNA fragmentation was measured using comet assay and Flow cytometric evaluation with Acridine Orange (FCEAO). The degree of methylation of nine genes was determined by bisulfite pyrosequencing of genomic DNA. Result(s) Analysis of three maternally imprinted genes (LIT1, SNRPN, MEST), two paternally imprinted genes (MEG3, H19), two repetitive elements (ALU, LINE1), one spermatogenesis-specific gene (VASA) and one gene associated with male infertility (MTHFR) in semen samples demonstrated no alteration in methylation pattern regardless of duration of cryopreservation. Conclusion(s) The lack of any changes in the sub-fraction of the genome examined in our study, implies that sperm DNA methylation is unaffected by cryopreservation and suggests that this daily clinical routine is safe in terms of DNA methylation.

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Section: Introductionmentioning

confidence: 72%

Routine cryopreservation of spermatozoa is safe — Evidence from the DNA methylation pattern of nine spermatozoa genes

Kläver¹,

Bleiziffer²,

Redmαnn³

et al. 2012

J Assist Reprod Genet

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“…Genetic defects, especially chromosomal abnormalities, are the most common causes of spontaneous miscarriage during the first trimester. Chromosomal abnormalities occur in approximately 60% of such cases [2][3][4]. The majority of firsttrimester miscarriages exhibit aneuploidies.…”

Section: Introductionmentioning

confidence: 99%

Comparison of chromosomal abnormality rates in ICSI for non-male factor and spontaneous conception

Bingöl

Abıke

Gedikbaşı³

et al. 2011

J Assist Reprod Genet

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Objective To compare cytogenetic data of first-trimester missed abortions in intracytoplasmic sperm injection (ICSI) for non-male factor-mediated and spontaneous pregnancies. Methods Using karyotype analysis, we conducted a retrospective cohort trial of missed abortions following ICSI for non-male factor and spontaneous pregnancies. Patients experienced missed abortions during the first 12 weeks of pregnancy. Dilation and curettage procedure was performed followed by cytogenetic evaluations. Two patient groups were created: ICSI (n=71) and spontaneous pregnancies (n= 81). At least 20 GTG-banded metaphases were analyzed in each case for cytogenetic analyses. Statistical analyses were performed using NCSS 2007 Statistical Program software. The significance level and confidence interval for all analyses were set to p<0.05 and a 95% confidence interval, respectively. Results A total of 49.3% (75/152) of the miscarriages were cytogenetically abnormal among the patients. We detected cytogenetically abnormalities in 47.9% (34/71) of the ICSI group and 50.6% (41/81) of the control group, which were not statistically significant differences (p=NS). The sex chromosome abnormalities were similar between the ICSI and control groups (p=NS). The most prevalent abnormalStatement for authors submitting original research: The responsible authors at the institute where the work has been carried out have approved the enclosed manuscript "Comparison of Chromosomal Abnormality Rates in ICSI for Non-Male Factor and Spontaneous Conception". Authors certify that none of the materials in this manuscript has been published previously in any form and that none of this material is currently under consideration for publication elsewhere. All authors have participated sufficiently in the intellectual content, the analysis of data and the writing the manuscript to take public responsibility for it.Capsule The chromosomal anomalies and aneuploidy rates following ICSI for non-male factor were similar to those following natural conception. Assist Reprod Genet (2012) 29:25-30 DOI 10.1007 ities that were observed in the ICSI and control groups with first-trimester pregnancy loss were trisomy (n=42; 27.6%), Turner syndrome (45, X0, n=13; 8.6%), triploidy (n=13; 8.6%), 48 chromosomes (n=5; 3.3%), and mixed chromosomal abnormalities (n=3; 1.2%). In addition, the karyotypes were similar between the ICSI and control groups (p=NS). We observed increases in fetal aneuploidy rates with increased maternal age (<30 years=23.9% vs. 31-34 years=37.0% vs. 35-39 years=82.9% vs. >39 years= 90.9%). However, the observed increases in fetal aneuploidy rates were not statistically significant (p=NS). Conclusion The aneuploidy rates and sex chromosome anomalies following ICSI for non-male factor were similar to those following natural conception.

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“…Approximately one in four women will have an early pregnancy failure during her lifetime [4]. Miscarriages in pregnancies achieved by assisted reproductive technology (ART) procedures may be slightly more frequent than in general population and occur in 16% -30% of patients with confirmed pregnancy [1,[5][6][7]. The major factor affecting this percentage is the mother's age.…”

Section: Introductionmentioning

confidence: 99%

“…The major factor affecting this percentage is the mother's age. Women ≤ 35 years old have a probability rate of 28.5%, compared to 38.6% in women ≥ 35 years old [1,5,7]. Additional risk factors include smoking and poor quality embryos, which double and triple the risk of a miscarriage respectively [6].…”

Section: Introductionmentioning

confidence: 99%

“…Success in either treatment is defined by abstaining from repeated surgical intervention or surgical treatment for patients who initial used the medical approach within the 30 days after initial treatment [4]. Previous studies have projected the overall expectant management of EPL to have a success rate of 25% (4,7). Surgically, dilatation and curettage (D & C) has been a commonly accepted treatment for first trimester pregnancy loss; however this procedure contains an inherent risk of complications not to be underestimated.…”

Section: Introductionmentioning

confidence: 99%

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Medical versus surgical treatment for early pregnancy loss in infertile patients: Which approach facilitates more rapid return to subsequent treatment cycle?

Vallejo¹,

Cotton²,

Lee³

et al. 2012

OJOG

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Incidence of spontaneous abortion among pregnancies produced by assisted reproductive technology

Cited by 130 publications

References 40 publications

Routine cryopreservation of spermatozoa is safe — Evidence from the DNA methylation pattern of nine spermatozoa genes

Routine cryopreservation of spermatozoa is safe — Evidence from the DNA methylation pattern of nine spermatozoa genes

Comparison of chromosomal abnormality rates in ICSI for non-male factor and spontaneous conception

Medical versus surgical treatment for early pregnancy loss in infertile patients: Which approach facilitates more rapid return to subsequent treatment cycle?

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