Incidence of Fig Leaf Mottle-associated Virus and Fig Mosaic Virus in Eastern 
 Province of Saudi Arabia

Alhudaib, Khalid

doi:10.3923/ijv.2012.128.132

Cited by 14 publications

(17 citation statements)

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“…In the present study, symptomatology identification of the FMV causes symptoms on leaves was performed. Our results were confirmed by other results obtained earlier by several investigators (Kitajima et al, 2003;Elbeaino et al, 2007;Alhudaib, 2012). Most of nursery fig trees were brought from Lebanon, Jordan and Syria.…”

Section: Analysis Of Amplified Productssupporting

confidence: 93%

“…Amplification confirms the presence of FMV infection in this plant. Like the present observations, Alhudaib (2012) and Walia et al (2014) also detected specific sequence of FMV in RNA extracts of infected plants by PCR. No specific product occurred for healthy material and no bands were found when the PCR assay was attempted without the initial RT step.…”

Section: Rt-pcr Detectionsupporting

confidence: 88%

“…It is a member of family Moraceae (Watson and Dallwitz, 2004;Alhudaib, 2012). (Blodgett and Gomec, 1967).…”

Section: Introductionmentioning

confidence: 99%

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Partial Nucleotide Sequence of the Family Bunyaviridae Associated with a Mosaic-Diseased Fig. in North Egypt

Sallam¹,

Farag²,

Elbeshe³

et al. 2015

International J. of Virology

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Four different patterns of chlorotic blotches, mosaic, yellowish mottling and deformation were observed and collected in four fig-growing areas of North Egypt from cv. Sultany farms. Fig mosaic virus like symptoms were successful purified through transmission an eriophyid mite, Aceria ficus Cotte to healthy potted rooted cuttings grown under glasshouse conditions. Young leaves appearing typical symptoms caused by FMV. Electron microscope observations of thin-sectioned tissues from symptomatic leaves showed Double Membrane Bodies (DMBs) characteristic for FMV particles compared with symptomless leaves. Viral particles belong to family of Bunyaviridae. The RT-PCR performed on total RNA extracted from leaf tissue infected with the studied virus confirmed the specificity of the primers of RNA-dependent RNA polymerase (RdRp) gene for RNA-dependent RNA polymerase. The size of the PCR amplified fragment was 302 bp. The sequence and phylogenetic analysis indicated that the Egyptian FMV isolate was closely related to other FMV isolates, especially the Italian and Arkansas ones with 80%similarity. The genetic relationship between the RdRp gene for the Egyptian isolate of FMV with other FMV sequences on the Genbank was studied.

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Section: Analysis Of Amplified Productssupporting

confidence: 93%

Section: Rt-pcr Detectionsupporting

confidence: 88%

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Partial Nucleotide Sequence of the Family Bunyaviridae Associated with a Mosaic-Diseased Fig. in North Egypt

Sallam¹,

Farag²,

Elbeshe³

et al. 2015

International J. of Virology

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“…Also, large quasi-spherical double membrane-bound particles DMPs of 100~150 nm in diameter were showed in purified extracted from tissues having yellow mosaic and malformation ( Figure 2D). This result is supported by (Appiano et al, 1995;Elbeaino et al, 2009c;Alhudaib, 2012), they observed similar results for the infection with FMV results in distinct double-membrane bodies or particles, called DMBs or DMPs, 90-200 nm in diameter in the cytosol of infected parenchyma cells. Comparable large quasi-spherical DMBs of 100-150 nm in diameter were located in the cytoplasm of parenchyma cells.…”

Section: Field Survey and Rt-pcr Detectionsupporting

confidence: 69%

Serological and Molecular Detection of Viruses Infecting Fig to Identify the Virus-Free Plants

Soliman¹

2018

J microb biotech food sci

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Fig (Ficus carica L.) is one of the old and the most common fruit tree grown in all the Mediterranean countries, which is among those exposed to diseases and pathogens. A recent investigation indicated that fig mosaic disease was the main pathogenic agent. In this study, field surveys were conducted in different areas of Egypt. A total of 300 leaf samples were collected randomly from symptomatic and symptomless fig trees of six cultivars, and tested by RT-PCR for the presence of Fig mosaic virus (FMV), Fig leaf mottle-associated virus 1 (FLMaV-1), Fig leaf mottle-associated virus 2 (FMMaV-2), Fig mild mottle-associated virus (FMMaV) and Fig latent virus 1 (FLV-1), using specific sets of primers. About 84% of the samples tested were found to be infected by at least one virus. FLMaV-1 was the prevailing virus with a (78.3%) incidence followed by FMV (73.7%), FLMaV-2 (50%), FLV-1(40.3%) and FMMaV (35%). The highest infection rate was observed in Sultani, Asuani and Adasi-Ahmer (100%), followed by Kahramani (80%), Kadota (64%) and Black Mission (60%).Ultra thin section for infected fig plants showed a large double-membrane particles (DMPs) 100-150 nm in diameter were located in the cytoplasm of parenchyma cells. Fig mosaic virus-specific antiserum raised in rabbits, proved useful for its detection in fig tissues. The plantlets from meristem tip tissue culture were transplanted successfully. FMV could be detected through DAS-ELISA in the virus-free fig plantlets.

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“…In Iran, based on a 2012 survey, approximately 14.7% of the tested fig trees were infected with fig cryptic virus (FCV; family Partitiviridae , genus Alphacryptovirus ), FFkaV and FMV ( Ale-Agha and Rakhshandehroo, 2014 ). In Saudi Arabia, Alhudaib (2012) noted that FMD is caused by FLMaV-1 and FMV. Conversely, in Egypt, Elbeshehy and Elbeaino (2011) detected four viruses: FLMaV-1 (predominant), FMV, FLMaV-2 and FMMaV.…”

mentioning

confidence: 99%

Viruses Associated with Fig Mosaic Disease in Different Fig Varieties in Montenegro

Latinović

Radišek

Bajčeta³

et al. 2019

Plant Pathol J

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Incidence of Fig Leaf Mottle-associated Virus and Fig Mosaic Virus in Eastern Province of Saudi Arabia

Cited by 14 publications

References 0 publications

Partial Nucleotide Sequence of the Family Bunyaviridae Associated with a Mosaic-Diseased Fig. in North Egypt

Partial Nucleotide Sequence of the Family Bunyaviridae Associated with a Mosaic-Diseased Fig. in North Egypt

Serological and Molecular Detection of Viruses Infecting Fig to Identify the Virus-Free Plants

Viruses Associated with Fig Mosaic Disease in Different Fig Varieties in Montenegro

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