Partial Nucleotide Sequence of the Family Bunyaviridae Associated with a Mosaic-Diseased Fig. in North Egypt

Abstract

Four different patterns of chlorotic blotches, mosaic, yellowish mottling and deformation were observed and collected in four fig-growing areas of North Egypt from cv. Sultany farms. Fig mosaic virus like symptoms were successful purified through transmission an eriophyid mite, Aceria ficus Cotte to healthy potted rooted cuttings grown under glasshouse conditions. Young leaves appearing typical symptoms caused by FMV. Electron microscope observations of thin-sectioned tissues from symptomatic leaves showed Double Membrane Bodies (DMBs) characteristic for FMV particles compared with symptomless leaves. Viral particles belong to family of Bunyaviridae. The RT-PCR performed on total RNA extracted from leaf tissue infected with the studied virus confirmed the specificity of the primers of RNA-dependent RNA polymerase (RdRp) gene for RNA-dependent RNA polymerase. The size of the PCR amplified fragment was 302 bp. The sequence and phylogenetic analysis indicated that the Egyptian FMV isolate was closely related to other FMV isolates, especially the Italian and Arkansas ones with 80%similarity. The genetic relationship between the RdRp gene for the Egyptian isolate of FMV with other FMV sequences on the Genbank was studied.

Incidence, Molecular Detection, and Partial Nucleotide Sequencing of Some Viruses Causing Fig Mosaic Disease (FMD) on Fig Plants in Egypt

“…On the other hand, Ref. [ 3 ] stated that sequence and phylogenetic analysis revealed that the Egyptian FMV isolate was closely related to other FMV isolates, particularly the Italian and Arkansas isolates with 80% similarity; however, Ref. [ 2 ] reported that nucleotide analyses of the two viruses studied (FMV and FLMaV-2) revealed that the sequence of FMV amplicons generated from different infected plants had 100% identity between the two isolates and showed 86.8–90.2% identity with other isolates reported in the GenBank database for FMV strains, whereas the two FLMaV-2 strains were 92.2% identical and showed 86.8–94.4% identity with other strains reported in the GenBank database.…”

“…The common fig ( Ficus carica L., Moraceae ) was one of the first cultivated fruit crops in the Mediterranean region, which is now regarded as the world's leading fig-producing region [ 1 , 2 ], and it is very important in Egypt [ 3 ]. Fig fruits may be eaten fresh or dried and are known for their moderate laxative properties [ 4 , 5 ].…”