Incidence of Apoptotic Cells After Vitrification in Canine Ovarian Tissues

Hariya, Madoka; Suzuki, Hiroshi

doi:10.1274/jmor.33.69

Cited by 8 publications

(8 citation statements)

References 40 publications

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“…In the TUNEL analysis, DNA fragmentation in the Fr and Vi groups was not significantly different. From this parameter, a previous study which used dog ovaries did not find significant difference between follicular DNA fragmentation in the fresh and vitrified groups [24]. Conversely, another study obtained significant difference in follicular apoptosis between the vitrification group compared to the fresh group [21].…”

Section: Discussionmentioning

confidence: 64%

Primordial follicle survival of goat ovarian tissue after vitrification and transplantation on chorioallanthoic membrane

Widad

Nurdiati

Ayuandari

et al. 2020

Middle East Fertil Soc J

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Background Ovarian tissue vitrification is an alternative fertility preservation procedure for young female patients prior to gonadotoxic treatment. Primordial follicle loss might be a potential issue for vitrification and transplantation procedures. This study aimed to evaluate primordial follicle density and deoxyribonucleic acid (DNA) fragmentation in each stage of the preservation procedure of goat ovarian tissue. Follicle density and DNA fragmentation were examined microscopically after staining with hematoxylin eosin and TUNEL assay, respectively. Both parameters were compared between fresh, fresh-transplanted, vitrification, and vitrification-transplanted groups. Results A significant decrease was observed in the primordial follicle proportion after vitrification and transplantation compared to the primordial follicle proportion in the fresh group (88.09% vs 52.42%, p < 0.05, 95% CI 11.54, 66.94). There was no significant difference in DNA fragmentations of primordial follicles between each group (p > 0.05). Conclusions The vitrification and transplantation process of goat ovarian strips could cause the primordial follicles loss and DNA damage of the follicles. However, primordial follicles loss and DNA damage were not significantly different in each procedure.

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Section: Discussionmentioning

confidence: 64%

Primordial follicle survival of goat ovarian tissue after vitrification and transplantation on chorioallanthoic membrane

Widad

Nurdiati

Ayuandari

et al. 2020

Middle East Fertil Soc J

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“…Also, it is known that the permeability of cryoprotective agents, such as EG and DMSO, interacts with ovarian cells and can cause excessive cell dehydration and consequently death (Morais et al, 2019). Some protocols use the cryotube throughout the procedure (Ishijima et al, 2006(Ishijima et al, , 2009Hariya & Suzuki, 2016) or just in the storage of the material in LN (Ackermann et al, 2016;Fujihara et al, 2019;Jivago et al, 2018), which represent a variety of protocols. Jivago et al, (2018), using the SSV technique and cryotubes for storage, showed a significant reduction in the normality of primordial follicles, primary and secondary (transitional follicles were not analysed).…”

Section: Discussionmentioning

confidence: 99%

Vitrification of canine ovarian tissue using the Ovarian Tissue Cryosystem (OTC) device

Brandão

Alves²,

Brito

et al. 2021

Reprod Domestic Animals

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“…In dogs, successful cryopreservation of ovarian tissue began with xenografting in immunodeficient mice [ 93 ]. One of the most recognized obstacles after ovarian tissue transplantation is ischemic injury [ 94 , 95 , 96 ]. Delayed revascularization and ischemia appear to be responsible for follicular loss and apoptosis [ 96 , 97 , 98 ].…”

Section: Cryopreservation Of Gonadal Tissuesmentioning

confidence: 99%

Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs

2022

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Assisted reproductive techniques (ARTs), such as artificial insemination, in vitro fertilization, and cryopreservation of gametes/zygotes, have been developed to improve breeding and reproduction of livestock and for the treatment of human infertility. Their widespread use has contributed to improvements in human health and welfare. However, in dogs, only artificial insemination using frozen semen is readily available as an ART to improve breeding and control genetic diversity. A recent priority in sperm cryopreservation is the development of alternatives to egg yolk, which is widely used as a component of the sperm extender. Egg yolk can vary in composition among batches and is prone to contamination by animal pathogens. The latter can be a problem for international exchange of cryopreserved semen. Low-density lipoprotein and skim milk are promising candidates for use as extenders, to ensure fertility after artificial insemination. Although not tested for its effects on fertility following artificial insemination, polyvinyl alcohol may also be a useful alternative to egg yolk as an extender. The development of cryopreservation techniques for canine embryos lags behind that for other mammals, including humans. However, given the success of non-surgical embryo transfer in 2011, studies have sought to refine this approach for practical use. Research on sperm cryopreservation has yielded satisfactory results. However, investigation of other approaches, such as cryopreservation of oocytes and gonadal tissues, remains insufficient. Techniques for the efficient induction of estrus may aid in the development of successful canine ARTs.

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Incidence of Apoptotic Cells After Vitrification in Canine Ovarian Tissues

Cited by 8 publications

References 40 publications

Primordial follicle survival of goat ovarian tissue after vitrification and transplantation on chorioallanthoic membrane

Primordial follicle survival of goat ovarian tissue after vitrification and transplantation on chorioallanthoic membrane

Vitrification of canine ovarian tissue using the Ovarian Tissue Cryosystem (OTC) device

Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs

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