Vitrification of canine ovarian tissue using the Ovarian Tissue Cryosystem (OTC) device

Brandão, Fabiana Aparecida Santilli; Alves, Kele Amaral; Brito, Danielle Cristina Calado de; Pereira, Leda Maria Costa; Morais, Glayciane Bezerra de; Ñaupas, Lucy Vanessa Sulca; Souza, Samara Silva de; Alves, Bênner Geraldo; Rodrigues, Ana Paula Ribeiro; Teixeira, Dárcio Ítalo Alves

doi:10.1111/rda.13979

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…In human, many studies on fertility preservation has been reported with a purpose to evade infertility caused from some kinds of malignant diseases (Donnez and Dolmans, 2017;Forman, 2018) and to restore from chemical therapies (Nichols-Burns et al, 2014). In animals, many studies has also been reported a purpose with preservation of genetic resource, preservation of fertility, and preliminary study for human in many species including pig (Borges et al, 2009) sheep (Fathi et al, 2011), goat (Rodrigues et al, 2004), cow (Kagawa et al, 2009), dog (Brandão et al, 2021), mouse (Salehnia et al, 2002;Terraciano et al, 2020), and non-human pri-…”

Section: Introductionmentioning

confidence: 99%

Fertility preservation in pig using ovarian tissues by vitrification method

Hwang

2022

J Anim Reprod Biotechnol

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Cryopreservation of porcine ovarian tissue by vitrification method is a promising approach to preserve genetic materials for future use. However, information is not enough and technology still remains in a challenge stage in pig. Therefore, the objective of present study was to determine possibility of vitrification method to cryopreserve porcine ovarian tissue and to confirm an occurrence of cryoinjuries. Briefly, cryoinjuries and apoptosis patterns in vitrified-warmed ovarian tissue were examined by histological evaluation and TUNEL assay respectively. In results, a damaged morphology of oocytes was detected among groups and the rate was significantly (p < 0.05) lower in vitrification group (25.8%) than freezing control group (67.7%), while fresh control group (6.6%) showed significantly (p < 0.05) lower than both groups. In addition, cryoinjury that form a wave pattern of tissues around follicles was found in the frozen control group, but not in the fresh control group as well as in the vitrification group. Apoptotic cells in follicle was observed only in freezing control group while no apoptotic cell was found in both fresh control and vitrification. Similarly, apoptotic patterns of tissues not in follicle were comparable between fresh control and vitrification groups while freezing control group showed increased tendency. Conclusively, it was confirmed that vitrification method has a prevention effect against cryoinjury and this method could be an alternative approach for cryopreservation of genetic material in pigs. Further study is needed to examine the viability of oocytes derived from vitrified-warmed ovarian tissue.

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Section: Introductionmentioning

confidence: 99%

Fertility preservation in pig using ovarian tissues by vitrification method

Hwang

2022

J Anim Reprod Biotechnol

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Effect of Different Vitrification Techniques on Viability and Apoptotic Index of Domestic Cat Testicular Tissue Cells

Carvalho,

Soares,

Leão

et al. 2023

Animals

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Vitrification is essential for successful tissue cryopreservation and biobanking in wild cats. This study aimed to compare different methods of vitrification (Ovarian Tissue Cryosystem—OTC, Straws—STW, and Solid Surface vitrification—SSV) for testicular fragment vitrification in tom cats. Testicular fragments were recovered from five adult tom cats and subjected to equilibrium vitrification using different cryovials and methods under the same conditions of vitrification solutions and cryoprotectants. The efficiencies of the methods were evaluated using histological analysis of spermatogonia and Sertoli cell nuclei, seminiferous tubular basement membrane detachment, and the gonadal epithelium shrinkage score scale. Cell viability was assessed using Hoechst PI and Terminal deoxynucleotidyl transferase nick end labeling (TUNEL) assay. The results showed that OTC is an effective vitrification method for maintaining the distinction between spermatogonia and Sertoli cells. OTC was similar to the control for basal membrane detachment parameters (p = 0.05). Epithelial shrinkage was low in the SSV group, which showed the highest percentage of viable cells among the vitrified groups (p = 0.0023). The OTC and SSV vitrification methods were statistically similar in terms of the percentage of TUNEL-positive cells (p = 0.05). Therefore, OTC and SSV provide favorable conditions for maintaining viable cat testicular tissue cells after vitrification.

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Recent Advancements in Vitrification Cryodevices for Gamete and Gonadal Tissue

et al. 2022

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Cryopreservation of gametes and gonadal tissue is nowadays primarily accomplished through vitrification. Variables such as cooling rate, viscosity and volume of vitrification solution are critical in gamete vitrification. In addition, sample size and stepwise exposure are also crucial for gonadal tissue vitrification. Recently a class of cryodevices has been developed to reduce the volume of vitrification solution so as to achieve higher cooling rates. Vitrification devices are classified as "open" or "closed" depending on whether the medium comes into direct contact with liquid nitrogen during the process. Examples of the open cryodevices for gamete vitrification are Cryotop, Cryolock, open pulled straw (OPS), etc., and closed devices are Vitrisafe, CryoTip, and high security vitrification kit. Similarly, for tissue vitrification open cryodevices used are needles, cryovials and closed devices used are Cryotissue, ovarian tissue cryosystem, etc. Among all the gamete cryodevices, Cryotop is unique and the best-selling micro-volume storage device. Use of this device has resulted in the highest number of babies born after embryo or oocyte vitrification. Another novel device, Kitasato vitrification system, is a vitrification solution absorber, which is similar to Cryotop but differs in one way, as it possesses a porous membrane that absorbs extra solution from the gamete. This review provides an update on the recent use of cryodevices for gamete and gonadal tissue vitrification.

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Vitrification of canine ovarian tissue using the Ovarian Tissue Cryosystem (OTC) device

Cited by 3 publications

References 24 publications

Fertility preservation in pig using ovarian tissues by vitrification method

Fertility preservation in pig using ovarian tissues by vitrification method

Effect of Different Vitrification Techniques on Viability and Apoptotic Index of Domestic Cat Testicular Tissue Cells

Recent Advancements in Vitrification Cryodevices for Gamete and Gonadal Tissue

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