Incidence of antibiotic-resistant Escherichia coli associated with frozen chicken carcasses and characterization of conjugative R plasmids derived from such strains

Caudry, S. D.; Stanisich, Vilmaa .

doi:10.1128/aac.16.6.701

Cited by 36 publications

(19 citation statements)

References 23 publications

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“…Such high rate of multidrug resistance may apparently be occurred due to indiscriminate usage of antimicrobial agents [1]. During slaughtering process multidrug resistant E. coli strains can be transferred often from the gut of poultry carcasses and contaminate its meat [8,24].…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial resistance pattern of Escherichia coli strains isolated from poultry farmers and poultry slaughterers in Morocco

Abdellah¹,

Fouzia²

2018

int. arab. j antimicrob. agents

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Aim: The objective of this study is to characterize the antibiotic resistance pattern of Escherichia coli isolates from the fecal samples of poultry workers (farmers and slaughterers), and to study the possible dissemination of resistant E. coli from poultry to humans. Methods:Sixty-four E. coli strains isolated from the fecal samples of poultry workers (33 from poultry farmers and 31 from poultry slaughterers) and 35 isolates from a control group workers were tested for antibiotic resistance by agar disk diffusion with 11 antimicrobial agents. Results:Resistance of E. coli isolated from poultry workers to tetracycline, ampicillin and norfloxacin were significantly (p < 0.05) higher than those isolated from the control group. All E. coli isolates were susceptible to cefotaxime, and most of them are susceptible to gentamicin, amikacin, cefoxitin and ertapenem. Multidrug resistance is alarmingly high in all groups, but was highest in poultry farmers isolates (84%) and poultry slaughterers isolates (80%). Approximately 25% of the E. coli isolates from poultry workers showed resistance to four or more antibiotics.

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Section: Discussionmentioning

confidence: 99%

“…During the slaughtering process of poultry birds, there can be occurring of fecal contamination through the guts of these birds with multiresistant bacteria especially E. coli [8,9]. The human intestinal tract is the ideal place where antibiotic resistant bacteria can transfer their resistance genes to the endogenous human flora [10].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance pattern of Escherichia coli strains isolated from poultry farmers and poultry slaughterers in Morocco

Abdellah¹,

Fouzia²

2018

int. arab. j antimicrob. agents

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“…At slaughter, resistant strains from the gut readily soil poultry carcasses and as a result poultry meats are often contaminated with multiresistant E. coli [8][9][10][11][12][13][14]; likewise eggs become contaminated during laying [15]. Hence, resistant faecal E. coli from poultry can infect humans both directly and via food.…”

Section: Introductionmentioning

confidence: 99%

Avian Colibacillosis and Salmonellosis: A Closer Look at Epidemiology, Pathogenesis, Diagnosis, Control and Public Health Concerns

Kabir

2010

IJERPH

352

272

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Avian colibacillosis and salmonellosis are considered to be the major bacterial diseases in the poultry industry world-wide. Colibacillosis and salmonellosis are the most common avian diseases that are communicable to humans. This article provides the vital information on the epidemiology, pathogenesis, diagnosis, control and public health concerns of avian colibacillosis and salmonellosis. A better understanding of the information addressed in this review article will assist the poultry researchers and the poultry industry in continuing to make progress in reducing and eliminating avian colibacillosis and salmonellosis from the poultry flocks, thereby reducing potential hazards to the public health posed by these bacterial diseases.

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“…Genes encoding such resistance are often found on large, transmissible R plasmids (20). Not surprisingly, multidrug-resistant APEC strains often carry conjugative plasmids (8). Interestingly, plasmids have been shown to be transferable from poultry to human isolates (23), suggesting that APEC strains and their plasmids might serve as reservoirs of resistance genes for bacteria that affect public health.…”

mentioning

confidence: 99%

DNA Sequence and Comparative Genomics of pAPEC-O2-R, an Avian Pathogenic Escherichia coli Transmissible R Plasmid

Johnson

Siek

Johnson

et al. 2005

Antimicrob Agents Chemother

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In this study, a 101-kb IncF plasmid from an avian pathogenic Escherichia coli (APEC) strain (APEC O2) was sequenced and analyzed, providing the first completed APEC plasmid sequence. This plasmid, pAPEC-O2-R, has functional transfer and antimicrobial resistance-encoding regions. The resistance-encoding region encodes resistance to eight groups of antimicrobial agents, including silver and other heavy metals, quaternary ammonium compounds, tetracycline, sulfonamides, aminoglycosides, trimethoprim, and beta-lactam antimicrobial agents. This region of the plasmid is unique among previously described IncF plasmids in that it possesses a class 1 integron that harbors three gene cassettes and a heavy metal resistance operon. This region spans 33 kb and is flanked by the RepFII plasmid replicon and an assortment of plasmid maintenance genes. pAPEC-O2-R also contains a 32-kb transfer region that is nearly identical to that found in the E. coli F plasmid, rendering it transferable by conjugation to plasmid-less strains of bacteria, including an APEC strain, a fecal E. coli strain from an apparently healthy bird, a Salmonella enterica serovar Typhimurium strain, and a uropathogenic E. coli strain from humans. Differences in the G؉C contents of individual open reading frames suggest that various regions of pAPEC-O2-R had dissimilar origins. The presence of pAPEC-O2-R-like plasmids that encode resistance to multiple antimicrobial agents and that are readily transmissible from APEC to other bacteria suggests the possibility that such plasmids may serve as a reservoir of resistance genes for other bacteria of animal and human health significance.Antimicrobial resistance among bacterial pathogens of food animals can complicate veterinary therapy. Resistant animal pathogens may also be a threat to human health if these resistant bacteria enter the food supply or otherwise serve as reservoirs of resistance genes for human pathogens. Transmissible R plasmids that encode multidrug resistance would seem a likely means by which animal pathogens could acquire resistance genes or transmit them to human pathogens. This study examines an R plasmid encoding multidrug resistance in an avian pathogenic Escherichia coli (APEC) isolate. APEC strains are important and prevalent bacterial pathogens of poultry (3) and are frequently found to be resistant to multiple antimicrobial agents (21, 37), including ampicillin, tetracycline, aminoglycosides, fluoroquinolones, quaternary ammonium compounds, and heavy metals (37). Genes encoding such resistance are often found on large, transmissible R plasmids (20). Not surprisingly, multidrug-resistant APEC strains often carry conjugative plasmids (8). Interestingly, plasmids have been shown to be transferable from poultry to human isolates (23), suggesting that APEC strains and their plasmids might serve as reservoirs of resistance genes for bacteria that affect public health. In the present study, the first complete sequence of a transmissible APEC R plasmid is presented and analyzed. Additionally, an...

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Incidence of antibiotic-resistant Escherichia coli associated with frozen chicken carcasses and characterization of conjugative R plasmids derived from such strains

Cited by 36 publications

References 23 publications

Antimicrobial resistance pattern of Escherichia coli strains isolated from poultry farmers and poultry slaughterers in Morocco

Antimicrobial resistance pattern of Escherichia coli strains isolated from poultry farmers and poultry slaughterers in Morocco

Avian Colibacillosis and Salmonellosis: A Closer Look at Epidemiology, Pathogenesis, Diagnosis, Control and Public Health Concerns

DNA Sequence and Comparative Genomics of pAPEC-O2-R, an Avian Pathogenic Escherichia coli Transmissible R Plasmid

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