Incidence of antibiotic resistance genotypes of Vibrio species recovered from selected freshwaters in Southwest Nigeria

Adesiyan, Ibukun M.; Bisi-Johnson, Mary A.; Okoh, Anthony I.

doi:10.1038/s41598-022-23479-0

Cited by 14 publications

(6 citation statements)

References 72 publications

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“…Similarly, a strong relationship between the tetracycline resistance phenotypes of V. parahaemolyticus isolates and the presence of ARG was also determined, as tetA was detected in 23 of 27 isolates that exhibited resistance to tetracycline. The overall level was found to be much higher than reported in other studies [ 45 , 51 ] in which the detection rate of tetA was 28% to 30% of tetracycline-resistant isolates. Meanwhile, tetA was undetected in all V. parahaemolyticus strains that exhibited resistance to tetracycline [ 5 , 52 ].…”

Section: Discussioncontrasting

confidence: 74%

Prevalence, multidrug resistance, and biofilm formation of Vibrio parahaemolyticus isolated from fish mariculture environments in Cat Ba Island, Vietnam

Nguyen,

Truong,

Thi

et al. 2024

PHRP

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Objectives: Vibrio parahaemolyticus is a major foodborne pathogen in aquatic animals and a threat to human health worldwide. This study investigated the prevalence, antimicrobial resistance, antimicrobial resistance genes (ARGs), and biofilm formation of V. parahaemolyticus strains isolated from fish mariculture environments in Cat Ba Island, Vietnam.Methods: In total, 150 rearing water samples were collected from 10 fish mariculture farms in winter and summer. A polymerase chain reaction assay was used to identify V. parahaemolyticus, its virulence factors, and ARGs. The antimicrobial resistance patterns and biofilm formation ability of V. parahaemolyticus strains were investigated using the disk diffusion test and a microtiter plate-based crystal violet method, respectively.Results: Thirty-seven V. parahaemolyticus isolates were recovered from 150 samples. The frequencies of the tdh and trh genes among V. parahaemolyticus isolates were 8.1% and 21.6%, respectively. More than 90% of isolates were susceptible to ceftazidime, cefotaxime, and chloramphenicol, but over 72% were resistant to ampicillin, tetracycline, and erythromycin. Furthermore, 67.57% of isolates exhibited multidrug resistance. The presence of ARGs related to gentamicin (aac(3)-IV), tetracycline (tetA) and ciprofloxacin (qnrA) in V. parahaemolyticus isolates was identified. Conversely, no ARGs related to ampicillin or erythromycin resistance were detected. Biofilm formation capacity was detected in significantly more multidrug-resistant isolates (64.9%) than non-multidrug-resistant isolates (18.9%).Conclusion: Mariculture environments are a potential source of antibiotic-resistant V. parahaemolyticus and a hotspot for virulence genes and ARGs diffusing to aquatic environments. Thus, the prevention of antibiotic-resistant foodborne vibriosis in aquatic animals and humans requires continuous monitoring.

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Section: Discussioncontrasting

confidence: 74%

Prevalence, multidrug resistance, and biofilm formation of Vibrio parahaemolyticus isolated from fish mariculture environments in Cat Ba Island, Vietnam

Nguyen,

Truong,

Thi

et al. 2024

PHRP

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“…Unlike the 31.2% MDR rate observed in this study, some other studies had recently reported 100% MDR rates in Vibrio isolates (Gxalo et al, 2021;Omoruyi & Ojubiaja 2022). These studies also differed from this (Odjadjare & Igbinosa, 2017;Adesiyan et al, 2022;Oko et al, 2022).…”

Section: Discussionmentioning

confidence: 75%

“…Other studies simply tested against ampicillin and reported rates ranging from 76.92% to 94.1% (Islam et al, 2021;Gxalo et al, 2021). A 2022 study however had a much lower rate of amoxicillin resistance (45%) reported (Adesiyan et al, 2022). Low rates of resistance were noted against gentamicin, ofloxacin and ciprofloxacin.…”

Section: Discussionmentioning

confidence: 99%

Potentially pathogenic multidrug-resistant <i>Vibrio</i> sp. isolated from dumpsites in Rivers State, Nigeria

Otokunefor,

Nwokolo,

Abara

et al. 2024

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Vibrio sp. is associated with several disease conditions. An assessment of the pathogenic potential of such strains is key in assessing the risk these organisms could pose to human health. This study therefore was aimed at analyzing the pathogenic potential of MDR Vibrio sp. isolated from select dumpsites in Port Harcourt, Rivers State. Two dumpsites in Port Harcourt, Rivers State were assessed for the presence of Vibrio sp. using standard microbiological techniques involving a pre-enrichment using alkaline peptone water, culture on Thiosulfate-citrate-bile salts-sucrose and biochemical characterisation. Following antimicrobial susceptibility testing using the standard Kirby Bauer disc diffusion method, pathogenic potential was assessed based on haemolytic activity and biofilm forming potential. Results showed that the highest rates of resistance were against cefuroxime (68.8%) while the lowest resistance rates (3.2%) were against the quinolone antibiotics (ofloxacin and ciprofloxacin). In total, 38 antibiograms were observed among the 93 isolates with AUG-CAZ-CRX-CXM noted as the most commonly occurring antibiogram. Only 31.2% (29/93) of isolates were multidrug resistant. A total of 47.3% of the test isolates exhibited pathogenic potential (either haemolytic ability or biofilm forming potential), with more (37.6%) exhibiting biofilm forming potential. A co-occurrence of both pathogenic characteristics was observed in only 6 (13.6%) isolates. Of the 93 isolates, a co-occurrence of MDR and pathogenic potential was observed in 16.1% of isolates (15/93). This study shows a moderate association between potentially pathogenic multidrug resistance Vibrio sp. and the sampled dumpsites indicating a low potential public health risk because of the non-pathogenic nature of strains isolated.

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“… 45/300 (15) bla NDM-1 NA [ 121 ] NA Vegetables, borehole and well water E. coli 8/100 (8) NA NA [ 122 ] NA Freshwater from river Vibrio spp. 28/315 (8.9) NA NA [ 123 ] NA Fish and Fish Storage Water Vibrio spp. 113/128 (88.3) NA NA [ 124 ] NA Rectal swab samples of poultry, beef, cattle, poultry and beef vendor table E. coli 78/138 (56.5) NA NA [ 125 ] Reunion island 2016–2017 Pig E. coli, K. pneumoniae 2/181 (1.1)* NA NA [ 126 ] Senegal 2010–2011 Human head lice A. baumannii 3** bla OXA23 -like NA [ 127 ] 2016 Fecal samples of Wild Chimpanzees, Termites K. pneumoniae 23** bla OXA-48 , bla KPC-2 ...…”

Section: Resultsmentioning

confidence: 99%

Carbapenem Resistance in Animal-Environment-Food from Africa: A Systematic Review, Recommendations and Perspectives

Dossouvi,

Ametepe

2024

IDR

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Background The World Health Organization (WHO) has classified carbapenem-resistant Enterobacteriaceae, Pseudomonas aeruginosa ( P. aeruginosa ), and Acinetobacter baumannii ( A. baumannii ) as high-priority pathogens, and carbapenem-resistant bacteria (CRB) have been reported to spread between humans, animals, and the environment. Objective This study aimed to conduct a systematic review of carbapenem resistance in animals, foods, and the environment on the African continent and to provide recommendations and perspectives for better prevention and control of carbapenem resistance in Africa. Results A total of 137 research articles collected from 2009 to 2023 were selected for this review, including articles reporting carbapenem-resistant bacteria in animals (81/137; 59.1%), the environment (66/137; 48.2%), and foods (26/137; 19%). Carbapenem-resistant bacterial species belonged to 31 genera and 17 families, including mainly Escherichia spp. (68/127; 53.5%); Klebsiella spp. (45/127; 35.4%); Pseudomonas spp. (20/127; 15.7%), Enterobacter spp. (19/127; 15%) and Acinetobacter spp. (15/127; 11.8%). The prevalence of CRBs by country ranged from 1.1% to 48.5%, and the pooled prevalence of CRBs isolated from animal-environment-food in Africa was 19.1% (2804/14,684; Standard Deviation = 15). Twenty carbapenemase families belonging to A, B, C, and D Ambler classes were reported, including mainly carbapenemase genes from bla OXA (44/84; 52.4%), bla NDM (34/84; 40.5%), bla SHV (23/84; 27.4%), bla KPC (22/84; 26.2%), bla VIM (19/84; 22.6%), and bla IMP (12/84; 14.3%) families. The reported mobile genetic elements (MGE) carrying carbapenemase-encoding genes included plasmids (16/19; 84.2%), integrons (3/19; 15.8%), transposons (3/19; 15.8%), and insertion sequences (2/19; 10.5%). bla OXA-48 was often carried by (60kb-65kb) IncL/M-type pOXA-48 plasmids, while bla NDM-5 was often carried by (45–50kb) IncX-type plasmids. Moreover, 25 articles investigated and reported virulent and hypervirulent CRBs that carried multiple virulence factors. Conclusion Animal-environment-food ecosystems would constitute reservoirs of CRBs involved in human infections. The One Health approach and constant collaboration between governments are necessary to drastically reduce the mortality rates linked to antimicrobial resistance.

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Incidence of antibiotic resistance genotypes of Vibrio species recovered from selected freshwaters in Southwest Nigeria

Cited by 14 publications

References 72 publications

Prevalence, multidrug resistance, and biofilm formation of Vibrio parahaemolyticus isolated from fish mariculture environments in Cat Ba Island, Vietnam

Prevalence, multidrug resistance, and biofilm formation of Vibrio parahaemolyticus isolated from fish mariculture environments in Cat Ba Island, Vietnam

Potentially pathogenic multidrug-resistant <i>Vibrio</i> sp. isolated from dumpsites in Rivers State, Nigeria

Carbapenem Resistance in Animal-Environment-Food from Africa: A Systematic Review, Recommendations and Perspectives

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