Incidence and Genetic Diversity of Grapevine Pinot gris Virus in California

Rwahnih, Maher Al; Diaz‐Lara, Alfredo; Arnold, K.; Cooper, Monica L.; Smith, Rhonda J.; Zhuang, George; Battany, Mark C.; Bettiga, Larry J.; Rowhani, Adib; Golino, Deborah A.

doi:10.5344/ajev.2020.20044

Cited by 7 publications

(6 citation statements)

References 33 publications

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“…Since the discovery of GPGV (Trichovirus) [16], the virus has been shown to be widespread in vineyards around the world, including in France [68], USA [69], Republic of Korea [70], Turkey [71], China [72], Canada [73], Germany [74], Pakistan [75], Spain [76], Brazil [77], Chile [78], Lebanon [79], Moldova [80], Georgia [81], Iran [82], Australia [83], Czech Republic [84], Algeria [85], England [86], Armenia [87], Bulgaria [88], Argentina [89], California [90], Japan [91], Belgium [92], Romania [93], Hungary [21], and Russia [47]. In 89% (34 out of 38) of the samples, we detected GPGV.…”

Section: Validation Of the Results Of Srna Hts By Rt-pcr And The Phylogenetic Analysis Of Detected Virusesmentioning

confidence: 99%

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

Navrotskaya

Porotikova

Yurchenko

et al. 2021

Viruses

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The use of high-throughput sequencing (HTS) technology has led to significant progress in the identification of many viruses and their genetic variants. In this study, we used the HTS platform to sequence small RNAs (sRNAs) of grapevine to study the virome. Isolation of RNA was performed using symptomatic grapevines collected from commercial vineyards in Krasnodar Krai in 2017–2018. To determine the viromes of vineyards, we used an integrated approach that included a bioinformatic analysis of the results of sRNA HTS and the molecular method RT-PCR, which made it possible to identify 13 viruses and 4 viroids. Grapevine leafroll-associated virus 4 (GLRaV-4), Grapevine Syrah Virus-1 (GSyV-1), Raspberry bushy dwarf virus (RBDV), Australian grapevine viroid (AGVd), and Grapevine yellow speckle viroid 2 (GYSVd-2) were identified for the first time in Russia. Out of 38 samples analyzed, 37 had mixed infections with 4–11 viruses, indicating a high viral load. Analysis of the obtained sequences of fragments of virus genomes made it possible to identify recombination events in GLRaV-1, GLRaV-2, GLRaV-3, GLRaV-4, GVT, GPGV, GRSPaV, GVA, and GFLV. The obtained results indicate a wide spread of the viruses and a high genetic diversity in the vineyards of Krasnodar Krai and emphasize the urgent need to develop and implement long-term strategies for the control of viral grapevine diseases.

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Section: Validation Of the Results Of Srna Hts By Rt-pcr And The Phylogenetic Analysis Of Detected Virusesmentioning

confidence: 99%

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

Navrotskaya

Porotikova

Yurchenko

et al. 2021

Viruses

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“…The absence of GRBV and GVT in the other 20 non-symptom-inducing isolates is also consistent with this assumption. Nevertheless, neither GRBV nor GVT seem necessary for symptom expression when we consider a study by Al Rwahnih et al in 2021 [ 28 ], who reported two asymptomatic vines harbouring both GPGV and GRBV, whereas all symptomatic vines were free of GVT. According to their study, GFLV was found as a co-infector with GPGV in all five symptomatic vines, whereas none of the asymptomatic vines were found infected with GFLV [ 25 ].…”

Section: Discussionmentioning

confidence: 96%

“…In addition to ubiquitous viruses and viroids such as GRSPaV, HSVd and GYSV-1, grapevines are regularly infected with GFkV, GLRaV-1, GLRaV-3 and GRBV [ 41 , 47 , 52 ]. Based on the few previous studies which tested for the presence of other viruses, symptom induction by GPGV did not co-segregate with any virus [ 28 , 35 , 36 ]. The only symptom-inducing isolate in this study, ON-10, co-infected the host with GPGV, GRBV, GVT, HSVd and GRSPaV.…”

Section: Discussionmentioning

confidence: 99%

“…It was not until 2019 that GPGV’s causal role in GLMD was established for the first time [ 3 ]. GPGV was reported in different Vitis vinifera cultivars from various grape-growing regions such as Algeria [ 4 ], Armenia [ 5 ], Argentina [ 6 ], Australia [ 7 ], Belgium [ 8 ], Brazil [ 9 ], Bulgaria [ 10 ], Canada [ 11 ], Chile [ 12 ], China [ 13 ], France [ 14 ], Germany [ 15 ], Greece [ 16 ], Iran [ 17 ], Italy [ 16 ], Japan [ 18 ], Lebanon and the Middle East [ 19 ], Moldavia [ 20 ], Pakistan [ 21 ], Poland [ 22 ], Slovakia and the Czech Republic [ 23 ], Slovenia [ 24 ], South Korea [ 25 ], Spain [ 26 ], Turkey [ 27 ] and the USA [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic and Evolutionary Studies of Grapevine Pinot Gris Virus Isolates from Canada

Vemulapati

McFadden-Smith

et al. 2023

Viruses

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This study investigated the phylogenetic relationship of grapevine Pinot gris virus (GPGV) isolates from Canada with GPGV isolates reported worldwide. Full-length genomes of 25 GPGV isolates representing the main four grape-growing regions in Canada (British Columbia, Ontario, Nova Scotia and Quebec) were sequenced and compared to genomes of 43 GPGV isolates representing eight countries and three continents. Phylogenetic analysis based on full genome sequences revealed an unambiguous separation of North American GPGV isolates with isolates from Europe and Asia. Within the North American clade, GPGV isolates from the USA segregated into a distinct subclade, whereas the relationships amongst GPGV isolates from different regions of Canada were not clearly defined. The phylogenetic analysis of the overlapping regions of MP and CP genes involving 169 isolates from 14 countries resulted in two distinctive clades, which were seemingly independent of their country of origin. Clade 1 included the majority of asymptomatic isolates (81% asymptomatic), whereas clade 2 was predominantly formed of symptomatic isolates (78% symptomatic). This research is the first study focused on the genetic variability and origin of GPGV in Canada.

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“…Total nucleic acid (TNA) extracts were prepared from 22 grapevine accessions as previously described [ 24 ]. TNA was isolated from petioles of selected grapevines.…”

Section: Methodsmentioning

confidence: 99%

Virus and Virus-like Pathogens in the Grapevine Virus Collection of Croatian Autochthonous Grapevine Cultivars

Vončina

Diaz‐Lara

Preiner

et al. 2022

Plants

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Grapevine collections play an important role, especially in the study of viruses and virus-like pathogens. In 2009, after an initial ELISA screening for eight viruses (arabis mosaic virus, grapevine fanleaf virus, grapevine fleck virus, grapevine leafroll-associated viruses 1, 2, and 3, and grapevine viruses A and B), a collection of 368 grapevine accessions representing 14 different Croatian autochthonous cultivars and containing single or mixed infection of viruses was established to further characterize the viral pathogens. Subsequently, Western blot, RT-PCR, cloning, and sequencing revealed that grapevine rupestris stem pitting-associated virus was frequently found in accessions of the collection, with isolates showing substantial genetic diversity in the helicase and coat protein regions. High-throughput sequencing of 22 grapevine accessions provides additional insight into the viruses and viroids present in the collection and confirms the fact that Croatian autochthonous grapevine cultivars have high infection rates and high virome diversity. The recent spread of “flavescence dorée” phytoplasma in Europe has not spared the collection. After the first symptoms observed in 2020 and 2021, the presence of phytoplasma was confirmed by LAMP in six grapevine accessions and some of them were lost. Single or multiple viruses and viroids, as well as own rooted grapevines in the collection, make the plants susceptible to various abiotic factors, which, together with the recent occurrence of “flavescence dorée”, makes the maintenance of the collection a challenge. Future efforts will be directed towards renewing the collection, as 56% of the original collection has been lost in the last 13 years.

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Incidence and Genetic Diversity of Grapevine Pinot gris Virus in California

Cited by 7 publications

References 33 publications

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

High-Throughput Sequencing of Small RNAs for Diagnostics of Grapevine Viruses and Viroids in Russia

Phylogenetic and Evolutionary Studies of Grapevine Pinot Gris Virus Isolates from Canada

Virus and Virus-like Pathogens in the Grapevine Virus Collection of Croatian Autochthonous Grapevine Cultivars

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