Incidence and Characterization of Staphylococcus aureus from the Tongues of Children

Miyake, Yoichiro; Iwai, Tsuyako; Sugai, Motoyuki; Miura, Kazuo; Suginaka, Hidekazu; Nagasaka, N

doi:10.1177/00220345910700070501

Cited by 59 publications

(49 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…5 Of concern was the small but significant number of MRSA isolates recovered from oral specimens. This may reflect the increasing reservoir of community MRSA, for example, 17% of nursing homes in one locality.…”

Section: Discussionmentioning

confidence: 99%

“…1 Some oral infections are caused at least in part by S. aureus, for example, angular cheilitis, 2 parotitis 3 and staphylococcal mucositis. 4 Furthermore there is now a growing body of evidence to suggest that staphylococci can be frequently isolated from the oral cavity of particular patient groups such as children, 5 the elderly 4 and some groups with systemic disease, such as the terminally ill, 6 rheumatoid arthritis 7 and patients with haematological malignancies. 8 The aim of this study was to identify the rate of S. aureus isolation from specimens submitted to a regional diagnostic oral microbiology laboratory over the period 1998-2000.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Staphylococcus aureus in the oral cavity: a three-year retrospective analysis of clinical laboratory data

Smith¹,

et al. 2003

View full text Add to dashboard Cite

Objective A retrospective analysis of laboratory data to investigate the isolation of Staphylococcus aureus from the oral cavity and facial area in specimens submitted to a regional diagnostic oral microbiology laboratory. Methods A hand search of laboratory records for a three-year period (1998)(1999)(2000) was performed for specimens submitted to the regional diagnostic oral microbiology laboratory based at Glasgow Dental Hospital and School. Data were collected from forms where S. aureus was isolated. These data included demographics, referral source, specimen type, methicillin susceptibility and clinical details. Results For the period 1998-2000, there were 5,005 specimens submitted to the laboratory. S. aureus was isolated from 1,017 specimens, of which 967 (95%) were sensitive to methicillin (MSSA) and 50 (5%) were resistant to methicillin (MRSA). The 1,017 specimens were provided from 615 patients. MRSA was isolated from 37 (6%) of patients. There was an increasing incidence of S. aureus with age, particularly in the >70 years age group. The most common specimen from which MSSA was isolated was an oral rinse (38%) whilst for MRSA isolates this was a tongue swab (28%). The clinical condition most commonly reported for MSSA isolates was angular cheilitis (22%). Erythema, swelling, pain or burning of the oral mucosa was the clinical condition most commonly reported for MRSA isolates (16%). Patients from whom the MSSA isolates were recovered were most commonly (55%) seen in the oral medicine clinic at the dental hospital, whilst patients with MRSA were more commonly seen in primary care settings such as nursing homes, hospices and general dental practice (51%). Conclusion In line with more recent surveys, this retrospective study suggests that S. aureus may be a more frequent isolate from the oral cavity than hitherto suspected. A small proportion of the S. aureus isolates were MRSA. There were insufficient data available to

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Staphylococcus aureus in the oral cavity: a three-year retrospective analysis of clinical laboratory data

Smith¹,

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Other authors have described the oral carriage of meticillin-resistant S. aureus (MRSA) (30,39). In addition, Miyake et al (22) found that 19% of the S.aureus isolates from oral samples produced exfoliative toxin and 40% of them enterotoxins.…”

Section: Figurementioning

confidence: 99%

Prevalence of "non-oral" pathogenic bacteria in subgingival biofilm of subjects with chronic periodontitis

Souto¹,

Andrade²,

Uzeda³

et al. 2006

Braz. J. Microbiol.

View full text Add to dashboard Cite

The oral cavity may act as a reservoir for several pathogens related to systemic infections. Therefore, the purpose of this study was to determine the prevalence and levels of pathogenic bacteria in the subgingival biofilm of chronic periodontitis lesions and healthy periodontal sites using the checkerboard DNA-DNA hybridization technique. 200 samples of subgingival biofilm from sites with periodontitis (probing pocket depth ≥ 4 mm and /or clinical attachment level ≥ 4 mm) and 200 samples from healthy sites of 14 patients with chronic periodontitis, as well as 200 samples from 3 periodontally healthy patients were obtained. The presence and levels of 11 pathogenic bacteria were determined using whole genomic DNA probes and the checkerboard method, computed for each site and then across sites within each subject group. Significance of differences in clinical and microbiological parameters among groups were examinated using the MannWhitney and Wilcoxon sign tests. The predominant species in all 600 samples included Corynebacterium diphtheriae, Enterococcus faecalis, Staphylococcus aureus, Acinetobacter baumannii and Escherichia coli. In general, most of the species were detected in greater prevalence and levels in sites with and without disease from patients with periodontitis in comparison to the periodontally healthy group. In particular, C. diphtheriae, E. coli, E. faecalis, Pseudomonas aeruginosa and S. aureus were significantly more prevalent and detected in higher counts in diseased sites of patients with periodontal disease compared to healthy subjects (p < 0.05). Clinical signs of disease presented a positive correlation with the species A. baumannii, Streptococcus pyogenes, E. coli, S. aureus and P. aeruginosa. In conclusion, "non-oral" pathogenic bacteria are detected in high prevalence and levels in periodontal sites of chronic periodontitis patients.

show abstract

“…Following work by Gordon et al (1966), studies have been conducted to analyse bacteria in the tongue biofilm, but most have targeted a limited number of bacterial species (Frisken et al, 1990;Miyake et al, 1991;van Winkelhoff et al, 1986). Comprehensive analyses of tongue biofilm microflora using culture methods or molecular biological methods have recently been reported (Hartley et al, 1996(Hartley et al, , 1999Kazor et al, 2003;Milnes et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Hydrogen sulfide-producing bacteria in tongue biofilm and their relationship with oral malodour

Washio

Sato

Koseki

et al. 2005

Journal of Medical Microbiology

157

135

View full text Add to dashboard Cite

The aims of this study were to identify hydrogen sulfide (H 2 S)-producing bacteria among tongue biofilm microflora and to investigate the relationship between bacterial flora and H 2 S levels in mouth air. Oral malodour levels in 10 subjects (age 21-56 years) were assessed by gas chromatography, and Breathtron and organoleptic scores. Based on these assessments, subjects were divided into two groups: an odour group and a no/low odour group. Tongue coatings were sampled and spread onto Fastidious Anaerobe Agar plates containing 0 . 05 % cysteine, 0 . 12 % glutathione and 0 . 02 % lead acetate, and were then incubated anaerobically at 37 8C for 2 weeks. Bacteria forming black or grey colonies were selected as H 2 S-producing phenotypes. The numbers of total bacteria (P , 0 . 005) and H 2 S-producing bacteria (P , 0 . 05) in the odour group were significantly larger than those in the no/low odour group. Bacteria forming black or grey colonies (126 isolates from the odour group; 242 isolates from the no/low odour group) were subcultured, confirmed as producing H 2 S and identified according to 16S rRNA gene sequencing. Species of Veillonella (38 . 1 % in odour group; 46 . 3 % in no/low odour group), Actinomyces (25 . 4 %; 17 . 7 %) and Prevotella (10 . 3 %; 7 . 8 %) were the predominant H 2 S-producing bacteria in both the odour and no/low odour groups. These results suggest that an increase in the number of H 2 S-producing bacteria in the tongue biofilm is responsible for oral malodour, although the bacterial composition of tongue biofilm was similar between the two groups.

show abstract

Incidence and Characterization of Staphylococcus aureus from the Tongues of Children

Cited by 59 publications

References 13 publications

Staphylococcus aureus in the oral cavity: a three-year retrospective analysis of clinical laboratory data

Staphylococcus aureus in the oral cavity: a three-year retrospective analysis of clinical laboratory data

Prevalence of "non-oral" pathogenic bacteria in subgingival biofilm of subjects with chronic periodontitis

Hydrogen sulfide-producing bacteria in tongue biofilm and their relationship with oral malodour

Contact Info

Product

Resources

About