Incidence and Characterization of Bean Common Mosaic Virus Isolates in Spanish Bean Fields

Sáiz, Margarita; Cde, Blas; Carazo, G.; Fresno, Juan Manuel Del; Romero, J.; Castro, S.

doi:10.1094/pd-79-0079

Cited by 21 publications

(18 citation statements)

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“…BnYDV was found as a new tentative species in south-eastern Spanish bean greenhouses (Segundo et al 2004a). Up to now, BCMV and BCMNV have been considered the predominant viruses in bean crops in Spain (Saiz et al 1995). Our data show that BCMV and BCMNV are uncommon in plastic greenhouses, probably because recent improvements in physical and chemical controls can eliminate the aphids that transmit those viruses, and commercial seeds are now certified as virus-free or virus-resistant.…”

Section: Discussionmentioning

confidence: 73%

“…Prior to our study, a preliminary study in Spanish bean fields identified several aphid transmitted viruses: Bean yellow mosaic virus (BYMV), Cucumber mosaic virus (CMV), Bean common mosaic virus (BCMV) and bean common mosaic necrotic virus (BCMNV; Saiz et al 1995). The first two were considered anecdotic; the latter two were considered to be the predominant viruses in Spain (Saiz et al 1995) and thus were considered common.…”

Section: Introductionmentioning

confidence: 99%

“…The first two were considered anecdotic; the latter two were considered to be the predominant viruses in Spain (Saiz et al 1995) and thus were considered common. In addition, two green bean viruses were reported in south-eastern Spain: Tomato spotted wilt virus (TSWV), detected by ELISA without any report on the plant symptoms (Lacasa et al 1991), and TYLCV , which produced a new disease called bean crumble leaf disease that caused severe economic losses in the green bean crops in 1998-1999.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence and incidence of viruses infecting green beans in south-eastern Spain

Segundo

Carmona

Sáez³

et al. 2008

Eur J Plant Pathol

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A study was conducted to determine the identity and prevalence of viruses in 455 greenhouses in the main Spanish green bean growing area. Directed surveys were conducted in 422 crops from 2000-2004 to collect samples from diseased plants displaying symptoms that could be attributed to viruses. The samples were analysed to detect any virus by means of dsRNA extraction, mechanical inoculation to test plants, as well as ELISA and/or RT-PCR tests to detect potyviruses, geminiviruses and viruses previously known to infect beans in Spain. Random surveys were conducted in the years 2002 and 2005 (in 21 and 12 greenhouses, respectively) to study the actual incidence of known viruses in the area. Symptoms were recorded in 23,108 plants from which 664 plants were collected and analysed by ELISA or RT-PCR. The results of the directed surveys showed that all the analyzed crops carried the cryptic virus Phaseolus vulgaris endornavirus (PVuV), whereas phytopathogenic viruses appeared in smaller percentages of the crops: Tomato yellow leaf curl virus (TYLCV) 20.4%, Southern bean mosaic virus (SBMV) 9.0%, Tomato spotted wilt virus (TSWV) 4.0%, and the new species Bean yellow disorder virus (BnYDV) that broke out in 2004 with occurrence values higher than 34.3% that year. From 2000-2004 an important decrease in TYLCV was observed, along with a slight increase in SBMV and a consistently low occurrence of TSWV. The results of the random surveys confirmed the increased occurrence of virus detected during the directed surveys, and furthermore demonstrated the percentage of incidence for each virus.

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Section: Discussionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Occurrence and incidence of viruses infecting green beans in south-eastern Spain

Segundo

Carmona

Sáez³

et al. 2008

Eur J Plant Pathol

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“…Both viruses are seedborne and transmitted by several aphid species in a non-persistent manner (Drijfhout, 1978). These viruses have been reported in common bean in many parts of the world including Europe (Drijfhout, 1978;Sáiz et al, 1995), U.S. (Kelly et al, 1983;Provvidenti et al, 1984), Canada (Tu, 1986), Mexico (Flores-Estévez et al, 2003) and Africa (Silbernagel et al, 1986;Spence & Walkey, 1995;Sengooba et al, 1997;Njau & Lyimo, 2000). BCMNV likely evolved more recently in the African continent (Spence & Walkey, 1995) and recombination has been observed between strains of BCMV and BCMNV (Silbernagel et al, 2001).…”

Section: Introductionmentioning

confidence: 93%

Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean

et al. 2005

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SummaryNecrotic strains of bean common mosaic potyviruses are becoming increasingly problematic in bean growing areas of Africa and Europe. Pyramiding epistatic resistance genes provides the most effective long-term strategy for disease control against all known strains of the virus. Indirect selection using tightly linked markers should facilitate the breeding of desired epistatic resistance gene combinations. In common bean, the most effective strategy for broad spectrum control of the bean common mosaic potyviruses is to combine I and bc-3 genes. We describe the use of near-isogenic lines and segregating populations from different gene pools combined with bulked segregant analysis to identify markers tightly linked with the recessive bc-3 gene that conditions resistance to all strains of bean common mosaic necrosis virus. We identified a RAPD marker, OG6 595 , linked at 3.7 cM from the bc-3, and the marker was used to confirm the location of bc-3 gene on bean linkage group B6. A codominant AFLP marker, E ACA M CGG -169/172 was identified and linked at 3.5 cM from the bc-3 and the AFLP and OG6 595 markers flanked the bc-3 gene. The AFLP marker was converted to the STS marker SE ACA M CGG -134/137 which showed co-segregation with the original AFLP marker. The 134 bp fragment associated with resistance was linked with the bc-3 gene present in a diverse group of bean genotypes except in two kidney bean lines. The OG6 595 marker mapped on B6 supported independence of bc-3 from the I gene located on B2, which provides the opportunity to readily combine both genes in a single bean cultivar for broad spectrum resistance to bean common mosaic potyviruses.

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“…BCMNV distribution is not as extensive as that of BCMV (Deligoz & Soken, 2011;Melgarejo et al, 2007;Petrovic et al, 2010;Sáiz et al, 1995). This is probably because BCMNV evolved from BCMV more recently (Gibbs, Ohshima, et al, 2008;Gibbs, Trueman, et al, 2008;Spence & Walkey, 1995).…”

Section: Geographic Distributionmentioning

confidence: 99%

Bean Common Mosaic Virus and Bean Common Mosaic Necrosis Virus

Worrall

Wamonje

Mukeshimana

et al. 2015

Advances in Virus Research

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Incidence and Characterization of Bean Common Mosaic Virus Isolates in Spanish Bean Fields

Cited by 21 publications

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Occurrence and incidence of viruses infecting green beans in south-eastern Spain

Occurrence and incidence of viruses infecting green beans in south-eastern Spain

Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean

Bean Common Mosaic Virus and Bean Common Mosaic Necrosis Virus

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