Incidence, Aggressiveness and<i>In Planta</i>Interactions of<i>Botrytis cinerea</i>and other Filamentous Fungi Quiescent in Grape Berries and Dormant Buds in Central Washington State

Dugan, Frank M.; Lupien, S. L.; Grove, Gary G.

doi:10.1046/j.1439-0434.2002.00774.x

Cited by 39 publications

(35 citation statements)

References 34 publications

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“…As mentioned, we have isolated other fungal genera that have been reported to control grapevine pathogens, such as Alternaria (A. alternata, A. tenuissima and A. arborescens). Taxa from this cosmopolitan group have been previously characterized as endophytes of grapevine (Polizzotto et al 2008), and reported to inhibit etiological agents of grapevine diseases like Plasmopara viticola (Musetti et al 2006) or Botrytis cinerea (Dugan et al 2002). Furthermore, other "classical" fungal antagonists like those from the genus Trichoderma were also isolated in our study with a total of 44 isolates, including T. aureoviride and T. harzianum.…”

Section: Discussionsupporting

confidence: 60%

The endophytic mycota associated with Vitis vinifera in central Spain

García¹,

Tello²

2010

Fungal Diversity

174

120

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This study investigates the diversity of fungal endophytes associated with several varieties of grapevines with differing modes of cultivation in the Madrid region (central Spain). Our goal is to screen for and identify new fungal biocontrol agents against vine diseases, especially those associated with young plants produced in nurseries. A total of 500 fungal strains representing 68 taxa from six locations were isolated and characterised. Differences regarding cultivar and plant part processed were analysed in terms of composition and relative abundance of species. Some of the more frequently isolated strains represented were Acremonium, Alternaria, Aureobasidium, Botryotinia, Cladosporium, Epicoccum, Fusarium, Gibberella, Nectria, Penicillium, Phoma and Trichoderma species. Botryosphaeria species and Phomopsis viticola were also frequently isolated and may be vine pathogens. Several species of Acremonium, Phoma (P. glomerata) and Chaetomium showed promising antagonistic activity at the laboratory scale.

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Section: Discussionsupporting

confidence: 60%

The endophytic mycota associated with Vitis vinifera in central Spain

García¹,

Tello²

2010

Fungal Diversity

174

120

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“…They showed varying effectiveness at inhibiting the pathogen. Only A. alternata, a known grapevine endophyte (12,18,25), was completely effective in inhibiting P. viticola sporulation on grapevine leaf disks in these trials. For this reason, we conducted further studies on the interaction between the pathogen and A. alternata.…”

Section: Discussionmentioning

confidence: 84%

“…alternata is a cosmopolitan species and has often been reported to inhabit the structures of substomatic chambers (18,39). It is well known that the P. viticola mycelium develops first in the substomatal air space, which subsequently becomes almost completely invaded by the growing hyphae (22).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Sporulation and Ultrastructural Alterations of Grapevine Downy Mildew by the Endophytic Fungus Alternaria alternata

Musetti¹,

Vecchione²,

Stringher³

et al. 2006

Phytopathology®

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One hundred twenty-six endophytic microorganisms isolated from grapevine leaves showing anomalous symptoms of downy mildew were tested on grapevine leaf disks as biocontrol agents against Plasmopara viticola. Among the 126 microorganisms, only five fungal isolates completely inhibited the sporulation of P. viticola; all of them were identified as Alternaria alternata. Ultrastructural analyses were carried out by transmission electron microscopy to observe cellular interactions between P. viticola and A. alternata in the grapevine leaf tissue. Cytological observations indicated that, even without close contact with A. alternata, the P. viticola mycelium showed severe ultrastructural alterations, such as the presence of enlarged vacuoles or vacuoles containing electron-dense precipitates. Haustoria appeared necrotic and irregularly shaped or were enclosed in callose-like substances. Therefore, a toxic action of A. alternata against P. viticola was hypothesized. To examine the production of toxic low-molecular-weight metabolites by A. alternata, we analyzed the fungal liquid culture by thin layer chromatography and proton magnetic resonance spectroscopy. The main low-molecular-weight metabolites produced by the endophyte were three diketopiperazines: cyclo(l-phenylalanine-trans-4-hydroxy-l-proline), cyclo(l-leucine-trans-4-hydroxy-l-proline), and cyclo(l-alanine-trans-4-hydroxy-l-proline). When applied at different concentrations to both grapevine leaf disks and greenhouse plants, a mixture of the three diketopiperazines was very efficacious in limiting P. viticola sporulation.

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“…Alternaria species are usually the principal fungal component of endophytic communities in phyllospheres, mostly due to their particular life style, producing highly melanised hyphae capable to resist and grow under intense UV radiations. Alternaria species (37 % of total isolates; 50 % in biological and 50 % in conventional), have shown antagonistic effects against Botrytis cinerea and grapevine downy mildew caused by Plasmopara viticola (Dugan et al 2002;Musetti et al 2006). The genus Epicoccum comprises 29 % of total isolates obtained in this study (average > 61 % in conventional mode).…”

Section: Discussionmentioning

confidence: 99%

Fungal endophytic communities associated to the phyllosphere of grapevine cultivars under different types of management

et al. 2016

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a b s t r a c tFungal endophytes present in different asymptomatic grapevine plants (Vitis vinifera L.) located in different vineyards within Alentejo, a highly important viticulture region in Portugal, were identified in this study. Sampled grapevine plants included the three most representative cultivars in the region, Syrah, Cabernet Sauvignon, and Aragonez, growing under two different modes of management, conventional and biological. Sixteen fungal taxa were identified through sequencing of the internal transcribed spacer region. Total number of endophytic fungi isolated showed significant differences both in management mode and in cultivars, with higher numbers in grapevines under conventional mode and from Syrah cultivar. The composition of fungal endophytic communities did not show significant differences among cultivars, but differences were observed between fungal communities isolated from grapevines under biological or conventional modes. The most fungal taxa isolated from grapevines cultivated under biological mode were Alternaria alternata, Cladosporium sp., and Nigrospora oryzae, and under conventional mode Botrytis cinerea, Epicoccum nigrum, and Epicoccum sp. These differences suggest that the different products used in grapevine production have impacts in fungal endophytic composition. Further investigation of the identified fungi with respect to their antagonistic characteristics and potential use in plant protection to ensure food safety is now in course.

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Incidence, Aggressiveness andIn PlantaInteractions ofBotrytis cinereaand other Filamentous Fungi Quiescent in Grape Berries and Dormant Buds in Central Washington State

Cited by 39 publications

References 34 publications

The endophytic mycota associated with Vitis vinifera in central Spain

The endophytic mycota associated with Vitis vinifera in central Spain

Inhibition of Sporulation and Ultrastructural Alterations of Grapevine Downy Mildew by the Endophytic Fungus Alternaria alternata

Fungal endophytic communities associated to the phyllosphere of grapevine cultivars under different types of management

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