Incenp and an Aurora-like kinase form a complex essential for chromosome segregation and efficient completion of cytokinesis

Kaitna, Susanne; Mendoza, Manuel; Jantsch, Verena; Glotzer, Michael

doi:10.1016/s0960-9822(00)00721-1

Cited by 288 publications

(302 citation statements)

References 35 publications

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“…The behavior of chromosomes in the DdINCENP null cells contrasted sharply with that of wild-type cells, which always displayed normal segregation of chromosomes (Graf et al, 2003; Figure 4; see Video 5). The mitotic and the cytokinetic defects displayed by the DdINCENP null cells were consistent with the observation in other organisms that INCENP plays important roles during mitosis (Kaitna et al, 2000;Adams et al, 2001b).…”

Section: Ddincenp Is Important For Mitosissupporting

confidence: 89%

“…It can stimulate the kinase activity of Aurora B and is also a substrate of Aurora B kinase (Kang et al, 2001;Honda et al, 2003). In both Drosophila and Caenorhabditis elegans, depletion of INCENP by RNAi causes failed chromosome congression at metaphase, mislocalization of Aurora B, and cytokinesis defects (Kaitna et al, 2000;Adams et al, 2001b). Also, in Xenopus mitotic egg extracts, immu-nodepletion of INCENP induced the formation of monopolar spindles (Sampath et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Two dominant-negative mutants of INCENP cause cytokinesis defects in mammalian cells (Eckley et al, 1997;Mackay et al, 1998). Additionally, in C. elegans embryos and Drosophila cultured cells, depletion of INCENP causes a cytokinesis defect (Kaitna et al, 2000;Giet and Glover, 2001). Some of the requirements for INCENP during cytokinesis may be mediated by its partner, Aurora B. Aurora B can phosphorylate MgcRacGAP, a RhoA GAP important for the completion of cytokinesis (Minoshima et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Contractile Ring-independent Localization of DdINCENP, a Protein Important for Spindle Stability and Cytokinesis

Chen

Lozanne

2006

MBoC

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Section: Ddincenp Is Important For Mitosissupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Contractile Ring-independent Localization of DdINCENP, a Protein Important for Spindle Stability and Cytokinesis

Chen

Lozanne

2006

MBoC

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“…lin through Aurora B phosphorylation (Kaitna et al 2000;Mishima et al 2004;Guse et al 2005). Finally, KIF20A is responsible for recruiting the CPC to the spindle midzone, thereby creating a pool of active Aurora B, which is essential for controlling the activity of KIF4A and KIF23 Fuller et al 2008).…”

mentioning

confidence: 99%

Cytokinesis in Animal Cells

D’Avino

Giansanti

Petronczki

2015

Cold Spring Harb Perspect Biol

177

226

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“…The Aurora kinases contain conserved C-terminal catalytic domains and divergent N-terminal domains and are classified into three families, A, B, and C (for review, see Nigg 2001). The Aurora B kinases interact with the conserved inner centromere protein (INCENP) (Kim et al 1999;Adams et al 2000Adams et al , 2001Kaitna et al 2000). Defects in INCENP localization disrupt Aurora B localization, suggesting that at least one function of the interaction may be to localize Aurora B to mitotic structures (Adams et al 2000).…”

mentioning

confidence: 99%

The budding yeast protein kinase Ipl1/Aurora allows the absence of tension to activate the spindle checkpoint

Biggins¹,

Murray²

2001

Genes Dev.

387

395

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The spindle checkpoint prevents cell cycle progression in cells that have mitotic spindle defects. Although several spindle defects activate the spindle checkpoint, the exact nature of the primary signal is unknown. We have found that the budding yeast member of the Aurora protein kinase family, Ipl1p, is required to maintain a subset of spindle checkpoint arrests. Ipl1p is required to maintain the spindle checkpoint that is induced by overexpression of the protein kinase Mps1. Inactivating Ipl1p allows cells overexpressing Mps1p to escape from mitosis and segregate their chromosomes normally. Therefore, the requirement for Ipl1p in the spindle checkpoint is not a consequence of kinetochore and/or spindle defects. The requirement for Ipl1p distinguishes two different activators of the spindle checkpoint: Ipl1p function is required for the delay triggered by chromosomes whose kinetochores are not under tension, but is not required for arrest induced by spindle depolymerization. Ipl1p localizes at or near kinetochores during mitosis, and we propose that Ipl1p is required to monitor tension at the kinetochore. The accurate propagation of genetic information depends on faithful chromosome segregation. Accurate chromosome segregation depends on the precise coordination of events in the chromosome cycle. When chromosomes replicate during S phase, linkage between the sister chromatids (cohesion) is established and must be maintained while chromosomes condense and align on the mitotic spindle. Chromosomes attach to the mitotic spindle by their kinetochores, specialized protein structures that are assembled on centromeric DNA sequences. Once all the chromosomes are correctly aligned on the mitotic spindle, sister chromatid cohesion must dissolve promptly at anaphase to allow the sister chromatids to segregate rapidly to opposite poles of the mitotic spindle. Defects in any of these steps can result in aneuploidy, a hallmark of tumor cells and some birth defects (Lengauer et al. 1997.The spindle checkpoint prevents cells from separating their sister chromatids until chromosome alignment is complete. The conserved components of the checkpoint include the Mad (Mad1-Mad3) proteins, Bub1 and Bub3, Mps1 (a protein kinase), and Cdc55 (Hoyt et al. 1991;Li and Murray 1991;Minshull et al. 1996;Weiss and Winey 1996;Wang and Burke 1997). A separate control, the Bub2-dependent checkpoint, monitors a second aspect of chromosome segregation, the delivery of DNA or a spindle pole body into the daughter cell (Alexandru et al. 1999;Fesquet et al. 1999;Fraschini et al. 1999;Li 1999). Spindle checkpoint defects are associated with genetic instability, and some human cancers contain mutant spindle checkpoint genes (Cahill et al. 1998;Takahashi et al. 1999) The spindle checkpoint monitors the interaction between kinetochores and microtubules. Spindle checkpoint proteins localize to kinetochores (Chen et al. 1996;Taylor and McKeon 1997;Bernard et al. 1998), and all known kinetochore and spindle defects that activate the checkpoint affect the i...

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Incenp and an Aurora-like kinase form a complex essential for chromosome segregation and efficient completion of cytokinesis

Abstract: ICP-1 and AIR-2 are part of a complex that is essential for chromosome segregation and for efficient completion of cytokinesis. We propose that this complex acts by promoting dissolution of sister chromatid cohesion and the assembly of the central spindle.

Cited by 288 publications

References 35 publications

Contractile Ring-independent Localization of DdINCENP, a Protein Important for Spindle Stability and Cytokinesis

Contractile Ring-independent Localization of DdINCENP, a Protein Important for Spindle Stability and Cytokinesis

Cytokinesis in Animal Cells

The budding yeast protein kinase Ipl1/Aurora allows the absence of tension to activate the spindle checkpoint

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