Contractile Ring-independent Localization of DdINCENP, a Protein Important for Spindle Stability and Cytokinesis

Chen, Qian; Li, Hui; Lozanne, Arturo De

doi:10.1091/mbc.e05-08-0704

Cited by 14 publications

(29 citation statements)

References 46 publications

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“…We showed previously that the distribution of DdINCENP at the cleavage furrow is modulated by myosin II, suggesting the ability of DdINCENP to interact with the actinmyosin cytoskeleton (Chen et al, 2006). Presumably, this ability is found at the amino terminus of DdINCENP be- cause this region was able to localize at the cleavage furrow ( Figure 5).…”

Section: Ddincenp 1-500 Interacts With the Actin Cytoskeletonmentioning

confidence: 79%

“…Instead of the broad cleavage furrow distribution observed in wild-type cells, DdINCENP localizes as a narrow band at the equatorial plane of myosin II null cells. These observations suggested that DdINCENP may be able to interact with the actomyosin cytoskeleton (Chen et al, 2006).…”

Section: Introductionmentioning

confidence: 90%

“…Green fluorescent protein (GFP)-DdINCENP and GFP-Kif12 were described previously (Lakshmikanth et al, 2004;Chen et al, 2006). Both plasmids carry a Geneticin (G418)-resistance cassette.…”

Section: Cell Culture Transformation and Constructsmentioning

confidence: 99%

“…Because both Kif12 and DdINCENP are found at the central spindle and cleavage furrow (Supplemental Movies 1 and 2) and because the loss of either protein leads to a similar late cytokinesis defect (Lakshmikanth et al, 2004;Chen et al, 2006), we explored the possibility of an interaction between these two proteins during cytokinesis. We first determined whether Kif12 was required for the localization of DdINCENP during mitosis or cytokinesis by comparing the localization of GFP-DdINCENP in wild-type and Kif12 null cells.…”

Section: The Mklp-related Protein Kif12 Localizes At the Cleavage Furmentioning

confidence: 99%

“…We showed previously that Dictyostelium INCENP (DdINCENP) localizes to the cortex of the cleavage furrow and that it is required for the abscission of the daughter cells (Chen et al, 2006). Importantly, the distribution of DdINCENP at the cleavage furrow is altered by the loss of myosin II.…”

Section: Introductionmentioning

confidence: 99%

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The Localization of Inner Centromeric Protein (INCENP) at the Cleavage Furrow Is Dependent on Kif12 and Involves Interactions of the N Terminus of INCENP with the Actin Cytoskeleton

Chen

Lakshmikanth

Spudich

et al. 2007

MBoC

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The inner centromeric protein (INCENP) and other chromosomal passenger proteins are known to localize on the cleavage furrow and to play a role in cytokinesis. However, it is not known how INCENP localizes on the furrow or whether this localization is separable from that at the midbody. Here, we show that the association of Dictyostelium INCENP (DdINCENP) with the cortex of the cleavage furrow involves interactions with the actin cytoskeleton and depends on the presence of the kinesin-6 -related protein Kif12. We found that Kif12 is found on the central spindle and the cleavage furrow during cytokinesis. Kif12 is not required for the redistribution of DdINCENP from centromeres to the central spindle. However, in the absence of Kif12, DdINCENP fails to localize on the cleavage furrow. Domain analysis indicates that the N terminus of DdINCENP is necessary and sufficient for furrow localization and that it binds directly to the actin cytoskeleton. Our data suggest that INCENP moves from the central spindle to the furrow of a dividing cell by a Kif12-dependent pathway. Once INCENP reaches the equatorial cortex, it associates with the actin cytoskeleton where it then concentrates toward the end of cytokinesis.

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Section: Ddincenp 1-500 Interacts With the Actin Cytoskeletonmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 90%

“…Green fluorescent protein (GFP)-DdINCENP and GFP-Kif12 were described previously (Lakshmikanth et al, 2004;Chen et al, 2006). Both plasmids carry a Geneticin (G418)-resistance cassette.…”

Section: Cell Culture Transformation and Constructsmentioning

confidence: 99%

Section: The Mklp-related Protein Kif12 Localizes At the Cleavage Furmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Localization of Inner Centromeric Protein (INCENP) at the Cleavage Furrow Is Dependent on Kif12 and Involves Interactions of the N Terminus of INCENP with the Actin Cytoskeleton

Chen

Lakshmikanth

Spudich

et al. 2007

MBoC

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Microtubule Organization inDictyostelium

Gräf

2009

Encyclopedia of Life Sciences

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Dictyostelium amoebae contain a radial array of microtubules emanating from a single microtubule‐organizing centre called centrosome that is bound to the cytosolic face of the nucleus. Their centrosome contains no centrioles but consists of a layered core surrounded by a corona harbouring microtubule nucleation centres. It duplicates in prophase of a closed mitosis and organizes a central spindle that drives centrosome separation and chromosome segregation. Though Dictyostelium microtubules are quite dynamic during mitosis, their length appears to be very stable during interphase. Microtubules are associated with a couple of conserved proteins ( microtubule‐associated protein , MAPs ), which are involved in centrosome biogenesis and the crosstalk of microtubule tips with the actin cell cortex. The latter becomes evident in cytokinesis, when centrosomes with their attached microtubules participate in the positioning of cleavage furrows. Key concepts: Dictyostelium amoebae contain a nucleus‐associated centrosome that serves as the only microtubule‐organizing centre. The Dictyostelium centrosome contains no centrioles, but consists of a three‐layered core structure surrounded by a microtubule‐nucleating corona. If compared to the three major plaques of the yeast spindle pole body, the entire core structure of the Dictyostelium centrosome appears equivalent to the central plaque, whereas the corona plays a similar role as the inner and outer plaques. Dictyostelium centrosomes duplicate at the onset of mitosis. Dictyostelium amoebae show a closed‐type of mitosis with a persisting nuclear envelope. Dictyostelium microtubules are quite dynamic during mitosis but show only little growth and shrinkage during interphase. Microtubule plus ends influence actin dynamics at the cell cortex. Dictyostelium amoebae are a useful model to study the role of the centrosome and microtubules in cell dynamics and disease.

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Microtubule Organisation inDictyostelium

Gräf

2015

Encyclopedia of Life Sciences

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Dictyostelium amoebae contain a radial array of microtubules emanating from a single microtubule‐organising centre called centrosome that is bound to the cytosolic face of the nucleus. Their centrosome contains no centrioles but consists of a layered core surrounded by a corona harbouring microtubule nucleation centres. It duplicates in prophase of a closed mitosis and organises a central spindle that drives centrosome separation and chromosome segregation. Dictyostelium microtubules exhibit a differential dynamic behaviour during interphase. Growth and shrinkage is observed only in the periphery but not in the pericentrosomal region. During mitosis, when centrosomes possess no corona, microtubules behave quite dynamically in formation of a central spindle and astral microtubules. Microtubules are associated with a couple of conserved proteins ( MAPs ), which are involved in centrosome biogenesis and the cross talk of microtubule tips with the actin cell cortex. The latter becomes evident in cytokinesis, when centrosomes with their attached microtubules participate in the positioning of cleavage furrows. Key Concepts Dictyostelium amoebae contain a nucleus‐associated centrosome that serves as the only microtubule‐organising centre. The Dictyostelium centrosome contains no centrioles, but consists of a three‐layered core structure surrounded by a microtubule‐nucleating corona. If compared to the three major plaques of the yeast spindle pole body, the entire core structure of the Dictyostelium centrosome appears equivalent to the central plaque, while the corona plays a similar role as the inner and outer plaques. Dictyostelium centrosomes duplicate at the onset of mitosis. Dictyostelium amoebae show a closed type of mitosis with a persisting nuclear envelope. Dictyostelium microtubules are quite dynamic during mitosis but show growth and shrinkage only in the periphery during interphase. Microtubule plus ends regulate actin dynamics at the cell cortex. Dictyostelium amoebae are a useful model to study the role of the centrosome and microtubules in cell dynamics and disease.

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Contractile Ring-independent Localization of DdINCENP, a Protein Important for Spindle Stability and Cytokinesis

Cited by 14 publications

References 46 publications

The Localization of Inner Centromeric Protein (INCENP) at the Cleavage Furrow Is Dependent on Kif12 and Involves Interactions of the N Terminus of INCENP with the Actin Cytoskeleton

The Localization of Inner Centromeric Protein (INCENP) at the Cleavage Furrow Is Dependent on Kif12 and Involves Interactions of the N Terminus of INCENP with the Actin Cytoskeleton

Microtubule Organization inDictyostelium

Microtubule Organisation inDictyostelium

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