Inactivation of viruses by coherent excitations with a low power visible femtosecond laser

Tsen, Kong-Thon; Tsen, Shaw Wei D; Chang, Chih Long; Hung, Chien Fu; Wu, T. C.; Kiang, Juliann G.

doi:10.1186/1743-422x-4-50

Cited by 47 publications

(45 citation statements)

References 8 publications

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“…Subsequent theoretical studies [2–4] led to the successful detection of the normal oscillations for low-frequency vibrational modes of bacteriophage M13 in water by Raman spectroscopy [5]. It was also demonstrated that by using a visible femtosecond laser, it is feasible to inactivate viruses such as the bacteriophage M13 through impulsive stimulated Raman scattering [6]. …”

Section: Introductionmentioning

confidence: 99%

Deformation of biological cells in the acoustic field of an oscillating bubble

Zinin

Allen

2009

Phys. Rev. E

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In this work we develop a theoretical framework of the interaction of microbubbles with bacteria in the ultrasound field using a shell model of the bacteria, following an approach developed previously [P. V. Zinin et al., Phys. Rev. E 72, 61907 (2005)]. Within the shell model, the motion of the cell in an ultrasonic field is determined by the motion of three components: the internal viscous fluid, a thin elastic shell, and the surrounding viscous fluid. Several conclusions can be drawn from the modeling of sound interaction with a biological cell: (a) the characteristics of a cell's oscillations in an ultrasonic field are determined both by the elastic properties of the shell the viscosities of all components of the system, (b) for dipole quadrupole oscillations the cell's shell deforms due to a change in the shell area this oscillation depends on the surface area modulus K A , (c) the relative change in the area has a maximum at frequency , where a is the cell's radius and ρ is its density. It was predicted that deformation of the cell wall at the frequency f K is high enough to rupture small bacteria such as E. coli in which the quality factor of natural vibrations is less than 1 (Q < 1). For bacteria with high value quality factors (Q > 1), the area deformation has a strong peak near a resonance frequency f K ; however, the value of the deformation near the resonance frequency is not high enough to produce sufficient mechanical effect. The theoretical framework developed in this work can be extended for describing the deformation of a biological cell under any arbitrary, external periodic force including radiation forces unduced by acoustical (acoustical levitation) or optical waves (optical tweezers).

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Section: Introductionmentioning

confidence: 99%

Deformation of biological cells in the acoustic field of an oscillating bubble

Zinin

Allen

2009

Phys. Rev. E

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“…The basic idea is that the entire energy of a pulse (which could be quite small) is delivered in a very short time, so if the average power is 10 mW and the pulse duration is 100 fs then the peak power is 100 GW which is sufficient to produce efficient 2-photon absorption. Recently, a series of studies have demonstrated the efficacy of a visible femtosecond laser or a near-infrared subpicosecond fiber laser on elimination of several kinds of viral species, including M13 bacteriophage , tobacco mosaic virus , HPV, and human immunodeficiency virus (HIV) [251–258]…”

Section: Other Light-based Anti-infectivesmentioning

confidence: 99%

Light based anti-infectives: ultraviolet C irradiation, photodynamic therapy, blue light, and beyond

Yin

Dai

Avci

et al. 2013

Current Opinion in Pharmacology

227

168

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Owing to the worldwide increase in antibiotic resistance, researchers are investigating alternative anti-infective strategies to which it is supposed microorganisms will be unable to develop resistance. Prominent among these strategies, is a group of approaches which rely on light to deliver the killing blow. As is well known, ultraviolet light, particularly UVC (200–280nm), is germicidal, but it has not been much developed as an anti-infective approach until recently, when it was realized that the possible adverse effects to host tissue were relatively minor compared to its high activity in killing pathogens. Photodynamic therapy is the combination of non-toxic photosensitizing dyes with harmless visible light that together produce abundant destructive reactive oxygen species (ROS). Certain cationic dyes or photosensitizers have good specificity for binding to microbial cells while sparing host mammalian cells and can be used for treating many localized infections, both superficial and even deep-seated by using fiber optic delivered light. Many microbial cells are highly sensitive to killing by blue light (400–470 nm) due to accumulation of naturally occurring photosensitizers such as porphyrins and flavins. Near infrared light has also been shown to have antimicrobial effects against certain species. Clinical applications of these technologies include skin, dental, wound, stomach, nasal, toenail and other infections which are amenable to effective light delivery.

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“…Now there are reports in the literature claiming novel biological effects of femtosecond pulse laser exposures in biological materials. Specifically, Tsen, et al, have reported measuring significant differences in inactivating exposures for bacteriophage M13, Tobacco Mosaic Virus, human papillomavirus, human immunodeficiency virus, Escherichia coli (E. coli), and a tissue culture line of human T-lymphocytes when irradiated with 80 fs pulses of 425 nm or 850 nm laser radiation [1][2][3] . The mechanism of interaction between the biomolecules and the ultrashort laser pulses is postulated to be impulsive stimulated Raman scattering (ISRS) 4 .…”

Section: Introductionmentioning

confidence: 98%

No effect of femtosecond laser pulses on DNA, protein, M13, or E. coli

Wigle

Holwitt

Noojin³

et al. 2011

SPIE Proceedings

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We were unable to reproduce published inactivation results, or show any interaction, between 90 femtosecond (fs) pulses of 850 nm or 425 nm laser radiation and buffer/water, DNA, protein, M13 bacteriophage or E. coli. Using agarose electrophoresis and polyacrylamide gel electrophoresis, we examined purified plasmid DNA (pUC19), bovine serum albumin, and DNA and coat proteins extracted from M13 following exposures to irradiances of up to 120 MW/cm 2 . We measured M13 viability using an assay for plaque-forming ability in soft agar after exposure to the same irradiances used for the protein and DNA experiments. Exposures of up 1 GW/cm 2 at 850 nm had no effect on the viability of E. coli as measured by a colony forming assay in soft agar. Peroxynitrite, known to be toxic, to cause single strand breaks in DNA, and fragment proteins in vitro gave positive results in all assays.

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Inactivation of viruses by coherent excitations with a low power visible femtosecond laser

Cited by 47 publications

References 8 publications

Deformation of biological cells in the acoustic field of an oscillating bubble

Deformation of biological cells in the acoustic field of an oscillating bubble

Light based anti-infectives: ultraviolet C irradiation, photodynamic therapy, blue light, and beyond

No effect of femtosecond laser pulses on DNA, protein, M13, or E. coli

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