Inactivation of Salmonella in liquid egg albumen by antimicrobial bottle coatings infused with allyl isothiocyanate, nisin and zinc oxide nanoparticles

Abstract

Aims: To develop an antimicrobial bottle coating effective at inhibiting the growth of Salmonella in liquid egg albumen (egg white) and reduce the risk of human Salmonellosis. Methods and Results: Four‐ounce glass jars were coated with a mixture of polylactic acid (PLA) polymer and antimicrobial compounds containing 100–500 μl allyl isothiocyanate (AIT), 250 mg nisin, 250 mg zinc oxide nanoparticles per jar or their combinations. The coated jars contained 100 ml of liquid egg white (LEW) inoculated with a three‐strain Salmonella enterica ssp. enterica cocktail at populations of 103 or 107 CFU ml−1 and stored at 10°C for 28 days. The PLA coating with 500 μl AIT completely inactivated 3 and 7 log CFU ml−1 of Salmonella after 7 and 21 days of storage, respectively. The PLA coating with 200 μl AIT in combination with 250 mg nisin reduced Salmonella populations to an undetectable level (<10 CFU ml−1) after 21 days of storage. Conclusions: PLA coatings containing AIT alone or in combination with nisin effectively inactivated salmonellae in LEW. Significance and Impact of the Study: This study demonstrated the commercial potential of applying the antimicrobial bottle coating method to liquid eggs and possibly other fluid food products.

The bactericidal effects of allyl isothiocyanate in water

“…Salmonella) [14,15], and that the MIC of AITC against E. coli ranges between 50 and 200 mg/l over 48 h [4,8].…”

Combination of pulsed electric field processing and antimicrobial bottle for extending microbiological shelf-life of pomegranate juice

“…In another study, the PLA coating with 500 μL AIT completely inactivated 3 and 7 log CFU/mL of Salmonella after 7 and 21 days of storage, respectively (Jin & Gurtler, 2011). However, to the best of the authors' knowledge, there are no reported studies combining PEF with antimicrobial bottles.…”

Effects of poly(ethylene oxide) and ZnO nanoparticles on the morphology, tensile and thermal properties of cellulose acetate nanocomposite fibrous film

“…The incorporation of metal nanoparticles (NPs) into polymer film matrices has been noted as the most promising method of achieving the latter goal. [1][2][3][4][5][6] The NPs offer an antimicrobial ability because of their extremely small particle sizes, which provide an extraordinarily high ratio of surface area to volume or surface area to weight as well as an innumerable number of active surfaces. Zinc oxide (ZnO) has been reported as an interesting potential substance for use in foods in antimicrobial applications.…”