Inactivation of Microorganisms in Model Biofilms by an Atmospheric Pressure Pulsed Non-thermal Plasma

Akishev, Yu. S.; Трушкин, Н. И.; Грушин, М. Е.; Petryakov, A. V.; Karalnik, Vladimir; Kobzev, E. N.; Kholodenko, V. P.; Чугунов, В. А.; Kireev, G. V.; Rakitsky, Yu.; Irkhina, I. A.

doi:10.1007/978-94-007-2852-3_12

Cited by 7 publications

(6 citation statements)

References 16 publications

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“…Plasmas generated in air and in contact with water are of the great interest in the plasma decontamination and plasma medicine community because they produce large quantities of reactive oxygen species (ROS) and reactive nitrogen species (RNS) that seem to be the most efficient biocidal agents in bio‐decontamination by plasma . Biological and therapeutic effects of air spark‐like discharges on tissues have been demonstrated .…”

Section: Introductionmentioning

confidence: 99%

Formation of ROS and RNS in Water Electro-Sprayed through Transient Spark Discharge in Air and their Bactericidal Effects

Machala

Tarabová

Hensel

et al. 2013

Plasma Process. Polym.

309

267

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Chemical and bactericidal effects induced by plasma in water upon electro‐spraying through DC‐driven positive transient spark discharge in air were investigated. Inactivation of E. coli was determined in dependence on pH (controlled by buffers) and correlated with chemical changes induced in water. Productions of hydrogen peroxide, nitrites, nitrates, peroxynitrites, and pH changes were determined, and the extent of oxidative stress induced in bacteria was evaluated. The degree of inactivation and oxidative damage of bacteria increased with the increasing acidity of the solution. Acidified nitrites interacting with hydrogen peroxide were determined as the most important bactericidal ROS/RNS agents in plasma‐treated water. A possible role of peroxynitrites, ozone, and metal nanoparticles is discussed.

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Section: Introductionmentioning

confidence: 99%

Formation of ROS and RNS in Water Electro-Sprayed through Transient Spark Discharge in Air and their Bactericidal Effects

Machala

Tarabová

Hensel

et al. 2013

Plasma Process. Polym.

309

267

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“…Undesirable effects of chemical washing include byproduct formation, safety concerns and negative effect on fresh produce quality. Akishev et al (2012) studied inactivation of atmospheric pressure NTP against two model biofilms generated by E. coli and Bacillus subtilis monocultures. The biofilms were grown on agar and on the surface of inert carriers, mild steel and polypropylene coupons.…”

Section: Biofilm-associated Foodborne Pathogensmentioning

confidence: 99%

“…Akishev et al . () studied inactivation of atmospheric pressure NTP against two model biofilms generated by E. coli and Bacillus subtilis monocultures. The biofilms were grown on agar and on the surface of inert carriers, mild steel and polypropylene coupons.…”

Section: Inactivation Of Biofilm‐associated Bacteria By Ntpsmentioning

confidence: 99%

Potential applications of nonthermal plasmas against biofilm-associated micro-organisms in vitro

Puligundla

Mok

2017

J Appl Microbiol

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Summary Biofilms as complex microbial communities attached to surfaces pose several challenges in different sectors, ranging from food and healthcare to desalination and power generation. The biofilm mode of growth allows microorganisms to survive in hostile environments and biofilm cells exhibit distinct physiology and behaviour in comparison with their planktonic counterparts. They are ubiquitous, resilient and difficult to eradicate due to their resistant phenotype. Several chemical‐based cleaning and disinfection regimens are conventionally used against biofilm‐dwelling micro‐organisms in vitro. Although such approaches are generally considered to be effective, they may contribute to the dissemination of antimicrobial resistance and environmental pollution. Consequently, advanced green technologies for biofilm control are constantly emerging. Disinfection using nonthermal plasmas (NTPs) is one of the novel strategies having a great potential for control of biofilms of a broad spectrum of micro‐organisms. This review discusses several aspects related to the inactivation of biofilm‐associated bacteria and fungi by different types of NTPs under in vitro conditions. A brief introduction summarizes prevailing methods in biofilm inactivation, followed by introduction to gas discharge plasmas, active plasma species and their inactivating mechanism. Subsequently, significance and aspects of NTP inactivation of biofilm‐associated bacteria, especially those of medical importance, including opportunistic pathogens, oral pathogenic bacteria, foodborne pathogens and implant bacteria, are discussed. The remainder of the review discusses majorly about the synergistic effect of NTPs and their activity against biofilm‐associated fungi, especially Candida species.

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“…The method of cold sterilization is widely used in processing medical instruments and materials [14,15]. The use of cold plasma in the food industry is introduced to a lower degree; however, the effectiveness of the bactericidal effect of LTAP on a wide range of causative agents of food spoilage has been studied and presented in literature quite extensive [16,17,18].…”

Section: Introductionmentioning

confidence: 99%

“…Helium and argon plasmas have the effective antimicrobial activity. The peculiarity of inert gas ionization is their easy flammability [18].…”

Section: Introductionmentioning

confidence: 99%

Assessment of quality and safety of pork treated with low-temperature atmospheric-pressure plasma

Moskalenko¹,

Kudryashov

Tikhonov³

et al. 2021

Teor. prakt. pererab. mâsa (Print)

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It is known that processing methods ensuring partial or full microbial inactivation are quite limited. Therefore, it is of great interest to develop technique and technologies allowing the effective action on microorganisms without a significant influence on product properties. The use of cold plasma can be one of the promising methods of meat product treatment by cold sterilization. The present work examines a possibility of chilled meat treatment with low-temperature atmospheric-pressure plasma to increase its stability to microbial spoilage and extend shelf life. To obtain low temperature plasma, the equipment developed by the designing department “Plasmamed” was used. Chilled meat was treated with low-temperature atmospheric-pressure argon plasma for 5, 10, 20 and 30 min. Samples were stored at a temperature of 2–4 °C for 10 days. Organoleptic indices, moisture weight fraction, changes in pH and water activity were analyzed before treatment and during storage. Sanitary microbiological analyses were carried out by the following indicators: quantity of mesophilic aerobic and facultative anaerobic microorganisms (QMAFAnM), the presence and quantity of coliforms, Salmonella, Escherichia coli, Listeria monocytogenes, Proteus. It was shown that meat cold treatment with argon plasma inhibited the development of mesophilic microorganisms. The colony forming units detected in the samples after ten days of storage were determined by the duration of exposure to plasma. It was proved that meat treatment for 15 and 30 min had the bactericidal effect and facilitated an improvement in meat color during storage. The organoleptic indices of the samples treated with plasma corresponded to the requirements of standards and approved consumer characteristics.

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Inactivation of Microorganisms in Model Biofilms by an Atmospheric Pressure Pulsed Non-thermal Plasma

Cited by 7 publications

References 16 publications

Formation of ROS and RNS in Water Electro-Sprayed through Transient Spark Discharge in Air and their Bactericidal Effects

Formation of ROS and RNS in Water Electro-Sprayed through Transient Spark Discharge in Air and their Bactericidal Effects

Potential applications of nonthermal plasmas against biofilm-associated micro-organisms in vitro

Assessment of quality and safety of pork treated with low-temperature atmospheric-pressure plasma

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