Inactivation of Bacterial Spore, Endotoxin, Lipid A, Normal Prion and Abnormal Prion by Exposures to Several Sorts of Gases Plasma

Shintani, Hideharu

doi:10.4265/bio.21.1

Cited by 23 publications

(24 citation statements)

References 35 publications

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“…The outer membrane presents phospholipids, lipopolysaccharides, lipoproteins [30–31]. The phospholipids are easily peroxidised during oxidative stress, such as during plasma treatment, compromising the structural integrity of the membrane [7, 31–35]. Gram-negative and Gram-positive bacteria have also different structure and nature of peptidoglycans [30].…”

Section: Discussionmentioning

confidence: 99%

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Innovative non-thermal plasma disinfection process inside sealed bags: Assessment of bactericidal and sporicidal effectiveness in regard to current sterilization norms

et al. 2017

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In this work, we developed a device capable to generate a non-thermal plasma discharge inside a sealed bag. The aim of this study was to assess the effectiveness of the oxygen, nitrogen and argon plasma sterilization on Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis spores according to the NF EN 556 Norm. Moreover the bag integrity which is a critical key to maintain the sterile state of items after the end of the process was verified by Fourier Transform Infrared (FTIR) and X-ray Photoelectron Spectrometry (XPS) analyses. After plasma treatments, the bacterial counting showed a 6 log reduction of P. aeruginosa and S. aureus in 45 min and 120 min respectively whatever the gas used and a 4 log reduction of B. subtilis spores in 120 min with only oxygen plasma. These results were confirmed by Scanning Electron Microscopy (SEM) observations showing altered bacteria or spores and numerous debris. Taking into account the studied microorganisms, the oxygen plasma treatment showed the highest efficiency. FTIR and XPS analyses showed that this treatment induced no significant modification of the bags. To conclude this non-thermal plasma sterilization technique could be an opportunity to sterilize heat and chemical-sensitive medical devices and to preserve their sterile state after the end of the process.

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Section: Discussionmentioning

confidence: 99%

“…g . reactor set-up, operating pressure, power, nature and flow rate of the gas…) [6, 8, 26, 29,31, 33, 40, 41, 43–45]; thus it is likely that more than one mechanism be involved in plasma microorganism inactivation.…”

Section: Discussionmentioning

confidence: 99%

Innovative non-thermal plasma disinfection process inside sealed bags: Assessment of bactericidal and sporicidal effectiveness in regard to current sterilization norms

et al. 2017

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confidence: 99%

“…CAP produces neither high temperatures, as do dry and wet heat, nor long-lived toxic gases, as do formaldehyde and ethylene oxide. As a consequence, CAP treatments tend to only minimally alter materials' natural properties and are increasingly used for sterilization of heat-sensitive medical instruments, hard surfaces, novel polymer packaging materials in the food industry (7,8), and biological surfaces such as animal and human tissues (4,5).Spores produced by some Bacillus species are a major cause of food spoilage and foodborne illness, as well as a number of other serious human diseases (9, 10). These spores can remain dormant for many years and are extremely resistant to a variety of environmental stresses, including antibiotics, heat, desiccation, radiation, and many toxic chemicals (11-13).…”

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confidence: 99%

Use of Raman Spectroscopy and Phase-Contrast Microscopy To Characterize Cold Atmospheric Plasma Inactivation of Individual Bacterial Spores

Wang

Doona

Setlow

et al. 2016

Appl Environ Microbiol

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Raman spectroscopy and phase-contrast microscopy were used to examine calcium dipicolinate (CaDPA) levels and rates of nutrient and nonnutrient germination of multiple individual Bacillus subtilis spores treated with cold atmospheric plasma (CAP). Major results for this work include the following: (i) >5 logs of spores deposited on glass surfaces were inactivated by CAP treatment for 3 min, while deposited spores placed inside an impermeable plastic bag were inactivated only ϳ2 logs in 30 min; (ii) >80% of the spores treated for 1 to 3 min with CAP were nonculturable and retained CaDPA in their core, while >95% of spores treated with CAP for 5 to 10 min lost all CaDPA; (iii) Raman measurements of individual CAP-treated spores without CaDPA showed differences from spores that germinated with L-valine in terms of nucleic acids, lipids, and proteins; and (iv) 1 to 2 min of CAP treatment killed 99% of spores, but these spores still germinated with nutrients or exogenous CaDPA, albeit more slowly and to a lesser extent than untreated spores, while spores CAP treated for >3 min that retained CaDPA did not germinate via nutrients or CaDPA. However, even after 1 to 3 min of CAP treatment, spores germinated normally with dodecylamine. These results suggest that exposure to the present CAP configuration severely damages a spore's inner membrane and key germination proteins, such that the treated spores either lose CaDPA or can neither initiate nor complete germination with nutrients or CaDPA. Analysis of the various CAP components indicated that UV photons contributed minimally to spore inactivation, while charged particles and reactive oxygen species contributed significantly. IMPORTANCEMuch research has shown that cold atmospheric plasma (CAP) is a promising tool for the inactivation of spores in the medical and food industries. However, knowledge about the effects of plasma treatment on spore properties is limited, especially at the single-cell level. In this study, Raman spectroscopy and phase-contrast microscopy were used to analyze CaDPA levels and kinetics of nutrient-and non-nutrient-germinant-induced germination of multiple individual spores of Bacillus subtilis that were treated by a planar CAP device. The roles of different plasma species involved in spore inactivation were also investigated. The knowledge obtained in this study will aid in understanding the mechanism(s) of spore inactivation by CAP and potentially facilitate the development of more effective and efficient plasma sterilization techniques in various applications.A mong low-temperature and nontoxic methods for microorganism inactivation, the application of cold atmospheric plasma (CAP) created by electrical discharge in a gas has attracted significant interest (1-5). CAP is a weakly ionized gas with a roughly zero net electrical charge and usually operates under atmospheric conditions below 40°C, with variations depending on the type of feed gas and the equipment configuration (6). Compared to conventional sterilization methods, such as w...

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“…[56789101112] In dentistry, CAP has opened a new painless way to prepare tooth cavities prior to restoration. It can be an effective tool for the treatment of dental caries and for composite restorations due to its properties of deactivation of pathogenic bacteria and modification of noninflammatory tissue.…”

Section: Introductionmentioning

confidence: 99%

Nonthermal plasma in dentistry: An update

Ranjan

Krishnamraju

Shankar

et al. 2017

J Int Soc Prevent Communit Dent

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Inactivation of Bacterial Spore, Endotoxin, Lipid A, Normal Prion and Abnormal Prion by Exposures to Several Sorts of Gases Plasma

Cited by 23 publications

References 35 publications

Innovative non-thermal plasma disinfection process inside sealed bags: Assessment of bactericidal and sporicidal effectiveness in regard to current sterilization norms

Innovative non-thermal plasma disinfection process inside sealed bags: Assessment of bactericidal and sporicidal effectiveness in regard to current sterilization norms

Use of Raman Spectroscopy and Phase-Contrast Microscopy To Characterize Cold Atmospheric Plasma Inactivation of Individual Bacterial Spores

Nonthermal plasma in dentistry: An update

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