Inactivation and mechanisms of chlorine dioxide on Nosema bombycis

Wang, Zhengyong; Fu-pin, Liao; Lin, Jianrong; Li, Wenchu; Zhong, Yang; Tan, Pei-chan; Ziran, Huang

doi:10.1016/j.jip.2009.11.007

Cited by 26 publications

(21 citation statements)

References 10 publications

(9 reference statements)

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“…In addition, HClO as a strong oxidizing agent could oxidize dehydrogenase and some other enzymes in respiration system of bacterial. The disinfection mechanism of HClO in SAEW was similar to the other strong oxidization disinfectant, such as ozone, hydrogen peroxide, chlorine dioxide and et al (Khadre & Yousef, 2001;Linley, Denyer, McDonnel, Simons, & Maillard, 2012;Uhm, Hong, Lee, & Park, 2009;Wang et al, 2010).…”

Section: Changes In Ttc-dehydrogenase Relative Activitymentioning

confidence: 86%

Disinfection efficacy and mechanism of slightly acidic electrolyzed water on Staphylococcus aureus in pure culture

Ding

Xuan

et al. 2016

Food Control

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Section: Changes In Ttc-dehydrogenase Relative Activitymentioning

confidence: 86%

Disinfection efficacy and mechanism of slightly acidic electrolyzed water on Staphylococcus aureus in pure culture

Ding

Xuan

et al. 2016

Food Control

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“…It can cause pebrine disease by vertical and horizontal transmission [ 2 , 3 ]. To some extent, chemical sanitizers such as lime powder, bleaching powder and ClO 2 can protect silkworms from this disease [ 4 ]; however, these methods polluted the environment and significantly increased the production costs of sericulture.…”

Section: Introductionmentioning

confidence: 99%

Engineered resistance to Nosema bombycis by in vitro expression of a single-chain antibody in Sf9-III cells

et al. 2018

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Nosema bombycis is a destructive, obligate intracellular parasite of the Bombyx mori. In this study, a single-chain variable fragment (scFv) dependent technology is developed for the purpose of inhibiting parasite proliferation in insect cells. The scFv-G4, which we prepared from a mouse G4 monoclonal antibody, can target the N. bombycis spore wall protein 12 (NbSWP12). Indirect immunofluorescence assays showed that NbSWP12 located mainly on the outside of the N. bombycis cytoskeleton, although some of it co-localized with β-tubulin in the meront-stage of parasites. When meront division began, NbSWP12 became concentrated at both ends of each meront. Western blotting showed that scFv-G4 could express in Sf9-III cells and recognized native NbSWP12. The transgenic Sf9-III cell line showed better resistance than the controls when challenged with N. bombycis, indicating that NbSWP12 is a promising target in this parasite and this scFv dependent strategy could be a solution for construction of N. bombycis-resistant Bombyx mori.

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“…ClO 2 is widely recognized as an excellent disinfectant for environmental sanitation, food safety, and agricultural production. Although there have been many reports on its sterilization mechanism, 10–18,33,34 we were able to successfully capture the real-time effects of aqueous ClO 2 at the individual Nb spore level using optical trapping and Raman spectroscopy. These data thus provide new insight into the inactivation mechanism of ClO 2 from the perspective of single-cell effects and molecular spectroscopy.…”

Section: Discussionmentioning

confidence: 99%

Laser Tweezers Raman Spectroscopy (LTRS) to Detect Effects of Chlorine Dioxide on IndividualNosema bombycisSpores

Zhang

Miao

Huang³

et al. 2019

Appl Spectrosc

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The microsporidium Nosema bombycis (Nb) causes pebrine, a fatal disease in sericulture. Nb is effectively killed by chlorine dioxide (ClO2); however, the precise killing mechanism remains unclear. We used laser tweezers Raman spectroscopy (LTRS) to monitor the action of ClO2 on individual Nb spores in real time. Raman peaks of ClO2 appeared in Nb spores, corresponding to decreased peaks of trehalose that gradually disappeared. A peak (1658 cm–1) corresponding to the protein α-helix significantly weakened while that (1668 cm–1) corresponding to irregular protein structures was enhanced; their intensities were negatively correlated in a certain time range and dependent on ClO2 concentration. The intensities of peaks at 782 cm–1 (nucleic acids) and 1004 cm–1 (phenylalanine of protein) did not change evidently even under extremely high ClO2 concentrations. Thus, ClO2 rapidly permeates the Nb spore wall, changing the protein secondary structure to lose biological function and destroy permeability, causing trehalose to leak out. These effects are ClO2 concentration-dependent, but no other obvious changes to biomacromolecules were detected. Single-cell analysis using LTRS is an effective method to monitor the action of chemical sporicides on microbes in real time, providing insight into the heterogeneity of cell stress resistance.

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Inactivation and mechanisms of chlorine dioxide on Nosema bombycis

Cited by 26 publications

References 10 publications

Disinfection efficacy and mechanism of slightly acidic electrolyzed water on Staphylococcus aureus in pure culture

Disinfection efficacy and mechanism of slightly acidic electrolyzed water on Staphylococcus aureus in pure culture

Engineered resistance to Nosema bombycis by in vitro expression of a single-chain antibody in Sf9-III cells

Laser Tweezers Raman Spectroscopy (LTRS) to Detect Effects of Chlorine Dioxide on IndividualNosema bombycisSpores

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