Inactivation and fragmentation of lectin from Bothrops leucurus snake venom by gamma irradiation

Nunes, Erika S.; Souza, Marco Antônio Andrade de; Vaz, Antônio Fernando de Melo; Coelho, Luana Cassandra Breitenbach Barroso; Aguiar, Jaciana dos Santos; Silva, T.G.; Guarnieri, M.C.; Melo, Ana Maria Mendonça de Albuquerque; Oliva, Maria Luiza Vilela; Correia, Maria T.S.

doi:10.1016/j.radphyschem.2011.12.048

Cited by 6 publications

(1 citation statement)

References 18 publications

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“…From a therapeutic point of view, the ability to inhibit tumoral growth is one the most relevant biological activities of venom lectins. Snake venom lectins exhibit several antitumoral effects, e.g., BlL from B. leucurus venom induced apoptosis in tumor cells through mitochondrial depolarization and permeability alterations (Nunes et al 2012, Aranda-Souza et al 2014, as did BjcuL from B. jararacussu (Damasio at al. 2014).…”

Section: An Acad Brasmentioning

confidence: 99%

Preliminary report on the hemagglutinating activity of the Scorpaena plumieri fish venom

Fiorotti

Soares

Borges

et al. 2022

An. Acad. Bras. Ciênc.

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The scorpionfi sh Scorpaena plumieri is one of the most venomous fi sh species in the Brazilian coast. Amongst many biological activities, the S. plumieri fi sh venom (SpV) promotes hemagglutination. Although this activity appears to be associated to the presence of C-type lectins in the venom, it has not yet been chemically or functionally characterized. In the present work we sought to advance the characterization of the hemagglutinating activity associated to this venom. By fractionating SpV through saline precipitation followed by size exclusion chromatography we obtained two purifi ed fractions -HF1 and HF3 -with Ca 2+ -dependent agglutinating activity against rabbit erythrocytes, which remained stable upon storage at 4 and -80 o C. HF1 and HF3 were bacteriostatic against Gram-positive bacteria (Staphylococcus aureus), displaying minimum inhibitory concentration (MIC) of 50 and 200 μg/mL, respectively. In addition, a resazurin-based viability assay revealed that both fractions, at doses up to 370 μg/ mL, were cytotoxic against tumor and non-tumor cell lines. Finally, a tendency towards edema formation could be detected when the fractions -particularly HF1 -were injected into mice footpads. We believe our data contribute to a better understanding of the biological properties of the so often neglected fi sh venoms.

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Section: An Acad Brasmentioning

confidence: 99%