Inactivating SARS-CoV-2 Using 275nm UV-C LEDs through a Spherical Irradiation Box: Design, Characterization and Validation

Trivellin, Nicola; Buffolo, Matteo; Onelia, Francesco; Pizzolato, Alberto; Barbato, Marco; Orlandi, Viviana Teresa; Vecchio, Claudia Del; Dughiero, Fabrizio; Zanoni, Enrico; Meneghesso, Gaudenzio; Crisanti, Andrea; Meneghini, Matteo

doi:10.20944/preprints202102.0588.v1

2021

DOI: 10.20944/preprints202102.0588.v1

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Inactivating SARS-CoV-2 Using 275nm UV-C LEDs through a Spherical Irradiation Box: Design, Characterization and Validation

Nicola Trivellin¹,

Matteo Buffolo²,

Francesco Onelia³

et al.

Abstract: We report on the design, characterization and validation of a spherical irradiation system for inactivating SARS-CoV-2, based on UV-C 275 nm LEDs. The system is designed to maximize ir-radiation intensity and uniformity, and can be used for irradiating a volume of 18 liters. To this aim: (i) several commercially available LEDs have been acquired and analyzed; (ii) a complete optical study has been carried out in order to optimize the efficacy of the system. (iii) The re-sulting prototype has been characterized… Show more

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Cited by 7 publications

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The Effectiveness of Far-Ultraviolet (UVC) Light Prototype Devices with Different Wavelengths on Disinfecting SARS-CoV-2

et al. 2021

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Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has emerged as a serious threat to human health worldwide. The inactivation of SARS-CoV-2 on object surfaces and in the indoor air might help to halt the COVID-19 pandemic. Far-ultraviolet light (UVC) disinfection has been proven to be highly effective against viruses and bacteria. To understand the wavelength and duration of UVC radiation required for SARS-CoV-2 inactivation, we examined the efficacy of UVC light prototype devices with the wavelengths of 275, 254, and 222 nm. The disinfection effectiveness was determined by cell-based assays including the median tissue culture infectious dose (TCID50) and an immunofluorescent assay on African green monkey kidney epithelial Vero E6 cells. Among the three prototypes, the UVC LED (275 nm) had the best virucidal activity with a log-reduction value (LRV) >6 after 10 s of exposure. The mercury lamp (254 nm) reached similar virucidal activity after 20 s of exposure. However, the excimer lamp (222 nm) showed limited anti-SARS-CoV-2 activity with a LRV < 2 after 40 s of exposure. Overall, in comparison, the UVC LED (275 nm) exhibited superior SARS-CoV-2 disinfection activity than the mercury lamp (254 nm) and the excimer lamp (222 nm).

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The Effectiveness of Far-Ultraviolet (UVC) Light Prototype Devices with Different Wavelengths on Disinfecting SARS-CoV-2

et al. 2021

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Use of Eco-Friendly UV-C LEDs for Indoor Environment Sanitization: A Narrative Review

et al. 2022

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Background: The current COVID-19 pandemic has demonstrated the enormous importance of maintaining good hygienic conditions in everyday indoor environments for the prevention of infectious diseases. This includes sanitization methods capable of significantly reducing the microbial load in the air and on surfaces. However, in line with the ecological transition, alternative systems for environmental sanitization with reduced environmental impact are urgently needed. The photocatalytic reaction using UV-C light-emitting diode (UV-C LED) lamps with short wavelengths, especially in the range of 200–280 nanometers (nm), can significantly reduce the microbial load, safeguarding the environment thanks to reduced energy consumption. The objective of this review is to describe the latest innovations in the use of UV-C LED technology in the sanitization of indoor environments, reporting the fundamental principles on which its activity relies. Methods: Two databases (PubMed, Web of Science), were searched, following PRISMA guidelines. Results: A total of 1348 publications were identified, of which 379 were assessed in detail and, of these, 16 were included in the review. Conclusions: This literature review highlighted that UV-C LEDs irradiation represents a valid, eco-sustainable sanitization method that could be exploited as an alternative to chemical compounds to contain indoor microbiological pollution in living and working environments.

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Surface Inactivation of Human Coronavirus by MACOMA™ UVA-TiO2 Coupled Photocatalytic Disinfection System

et al. 2022

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There is an immense healthcare challenge and financial pressure due to the COVID-19 pandemic caused by a newly identified human coronavirus, SARS-CoV-2. Effective COVID-19 prevention efforts in healthcare, home, and community settings highlight the need for rapid, efficient, and no-contact SARS-CoV-2 inactivation strategies. Here, we examined the photocatalytic and virucidal activity of the MACOMA™ TiO2 photocatalytic film activated by an UVA-LED-12V-367 nm (MA-717836-1) lamp against the HCoV-OC43, a member of the beta coronaviruses family, like SARS-CoV-2, using quantitative RT-qPCR and virus infectivity assays. The UVA radiation-responsive TiO2 film accelerated virus inactivation (decreased viral titer) compared to the uncoated glass surface when placed at a vertical distance of 1.2 feet (~14 inches) from virus samples for 10, 30, and 60 min. UVA-LED exposure for both 10 and 30 min effectively reduced the viral RNA copies and the infectious virus in samples on TiO2-coated surfaces compared to the control surfaces. Importantly, a 60 min exposure of samples on the TiO2 completely eliminated HCoV-OC43. These results confirmed that the MACOMA™ UVA/TiO2-based disinfection system provides a rapid and complete surface inactivation of tested human coronavirus in a human-safe manner and has great potential for limiting the virus spread in poorly ventilated as well as high-traffic public places.

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Inactivating SARS-CoV-2 Using 275nm UV-C LEDs through a Spherical Irradiation Box: Design, Characterization and Validation

Cited by 7 publications

References 14 publications

The Effectiveness of Far-Ultraviolet (UVC) Light Prototype Devices with Different Wavelengths on Disinfecting SARS-CoV-2

The Effectiveness of Far-Ultraviolet (UVC) Light Prototype Devices with Different Wavelengths on Disinfecting SARS-CoV-2

Use of Eco-Friendly UV-C LEDs for Indoor Environment Sanitization: A Narrative Review

Surface Inactivation of Human Coronavirus by MACOMA™ UVA-TiO2 Coupled Photocatalytic Disinfection System

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