“…In addition, by using adjuvants that differently modulate the maturation of Th cells, these vaccines might induce both humoral and cellular-mediated immunity [14] , [15] , [16] . The humoral immune response of silver catfish to inoculated bacteria [17] or purified antigens mixed with different types of adjuvant, including β-glucan, Montanide, Freund's complete and incomplete adjuvant, ODNs CpGs [18] , and inactivated Parapoxvirus ovis [39] has also been investigated; antigen-specific antibodies peak from 3 to 4 weeks post vaccination. However, there is no data regarding the early events following vaccination and protection to challenge with A. hydrophila .…”