Inactivated parapoxvirus ovis activates canine blood phagocytes and T lymphocytes

Abstract

Please cite this article as: Schütze, N., Raue, R., Büttner, M., Alber, G., Inactivated parapoxvirus ovis activates canine blood phagocytes and T lymphocytes, Veterinary Microbiology (2008Microbiology ( ), doi:10.1016Microbiology ( /j.vetmic.2009 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Enhanced burst rates by canine monocytes stimulated with iPPVO were observed and the 30 MHC-II expression on canine CD14+ monocytes was elevated following stimulation with iPPVO compared to the stabiliser control. Canine CD4+ T cells were activated for oligoclonal proliferation in response to iPPVO. This study shows that iPPVO is able to stimulate both phagocytotic and T-cell-dependent immune mechanisms in canine blood leukocytes. 35 keywords: cell activation, phagocytosis, oxidative burst, monocyte/macrophages, virus

Effects of inactivated Parapoxvirus ovis on polymorphonuclear leukocyte function and myeloperoxidase activity in horses

“…These results are consistent with the results of Schutze et al (2009), who investigated the immunostimulatory capacity of iPPVO on the innate immune system in vitro by evaluating the induction of the oxidative burst and the modulation of phagocytosis by canine blood leukocytes (PMNLs and monocytes) in dogs. After stimulation with iPPVO, the phagocytosis of fluorescein-isothiocyanate-labeled Listeria monocytogenes increased in canine blood, demonstrating that iPPVO stimulates PMNL functions in canine blood leukocytes.…”

“…However, in vitro experiments have shown that iPPVO stimulates both phagocytic and T-cell-dependent immune mechanisms directed against bacteria in canine blood leukocytes (Schutze et al 2009). In one study of horses, two commercial immunostimulants, iPPVO and inactivated Propionibacterium acnes, significantly enhanced the functions of phagocytic cells after ex vivo exposure to virulent Rhodococcus equi (Ryan et al 2010).…”

“…Inactivated P. ovis shows strong immunomodulatory activity in several species and is used in veterinary medicine as an immunostimulatory biological agent for the prevention and/or treatment of infectious diseases (Schutze et al 2009). PPVO stimulation has been shown to induce strong antiviral activity in laboratory animal models both in vitro and in vivo.…”

Specific antibodies induced by inactivated parapoxvirus ovis potently enhance oxidative burst in canine blood polymorphonuclear leukocytes and monocytes

“…In contrast, stimulation with PMA did not show any differences between sera from distinct time points. Interestingly, in the presence of immune serum not only monocytes but also PMN 295 responded to iPPVO stimulation with oxidative burst, whereas samples supplemented with serum before treatment showed iPPVO-induced burst activity exclusively in monocytes as recently described (Schütze et al, 2009). Consistent with the time course of the antibody levels (see Fig.…”

“…Moreover, it is of interest to explore if iPPVO-specific antibodies are able to 80 contribute to the known immunostimulatory properties of iPPVO. We have recently shown in vitro stimulation of canine phagocytes by iPPVO (Schütze et al, 2009). Inactivated PPVO potently enhanced phagocytosis of Listeria by monocytes and PMN.…”