Specific antibodies induced by inactivated parapoxvirus ovis potently enhance oxidative burst in canine blood polymorphonuclear leukocytes and monocytes

Schütze, Nicole; Raue, R.; Büttner, Mathias; Köhler, Gabriele; McInnes, Colin J.; Alber, Gottfried

doi:10.1016/j.vetmic.2009.07.027

Cited by 14 publications

(13 citation statements)

References 31 publications

(21 reference statements)

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“…Cytokines TNF-α, IL-1β, and IL-8 mediate the initial response of the innate immune system to a challenge, infection, or injury (31-33). TNF and IL-1β activate endothelial cells, attracting polymorphonuclear cells and monocytes to the site of inflammation and enhancing their movement through the blood.…”

Section: Discussionmentioning

confidence: 99%

Inactivated Parapoxvirus ovis induces a transient increase in the expression of proinflammatory, Th1-related, and autoregulatory cytokines in mice

Anziliero

Weiblen

Kreutz

et al. 2014

Braz J Med Biol Res

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The immunostimulatory properties of inactivated Parapoxvirus ovis (iPPVO) have long been investigated in different animal species and experimental settings. In this study, we investigated the effects of iPPVO on cytokine expression in mice after intraperitoneal inoculation. Spleen and sera collected from iPPVO-treated mice at intervals after inoculation were submitted to cytokine mRNA determination by real-time PCR (qPCR), serum protein concentration by ELISA, and interferon (IFN)-α/β activity by bioassay. The spleen of iPPVO-treated animals showed a significant increase in mRNA expression of all cytokines assayed, with different kinetics and magnitude. Proinflammatory cytokines interleukin (IL)-1β, tumor necrosis factor-alpha (TNF-α), and IL-8 mRNA peaked at 24 hours postinoculation (hpi; 5.4-fold increase) and 48 hpi (3- and 10-fold increases), respectively. A 15-fold increase in IFN-γ and 6-fold IL-12 mRNA increase were detected at 48 and 24 hpi, respectively. Increased expression of autoregulatory cytokines (Th2), mainly IL-10 and IL-4, could be detected at later times (72 and 96 hpi) with peaks of 4.7- and 4.9-fold increases, respectively. IFN-I antiviral activity against encephalomyocarditis virus was demonstrated in sera of treated animals between 6 and 12 hpi, with a >90% reduction in the number of plaques. Measurement of serum proteins by ELISA revealed increased levels of IL-1, TNF-α, IL-12, IFN-γ, and IL-10, with kinetics similar to those observed by qPCR, especially for IL-12 and IFN-γ. These data demonstrate that iPPVO induced a transient and complex cytokine response, initially represented by Th1-related cytokines followed by autoregulatory and Th2 cytokines.

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Section: Discussionmentioning

confidence: 99%

Inactivated Parapoxvirus ovis induces a transient increase in the expression of proinflammatory, Th1-related, and autoregulatory cytokines in mice

Anziliero

Weiblen

Kreutz

et al. 2014

Braz J Med Biol Res

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“…After stimulation with iPPVO, the phagocytosis of fluorescein-isothiocyanate-labeled Listeria monocytogenes increased in canine blood, demonstrating that iPPVO stimulates PMNL functions in canine blood leukocytes. Since then, iPPVO stimulation has been shown to enhance the phagocytic activity of canine neutrophils and monocytes in a dosedependent manner (Schutze et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Effects of inactivated Parapoxvirus ovis on polymorphonuclear leukocyte function and myeloperoxidase activity in horses

Ülgen¹,

Yarami̇s²,

Rayaman³

et al. 2014

Vet. Med. - Czech

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ABSTRACT:Immunomodulatory products have been used for years in veterinary medicine. Inactivated Parapoxvirus ovis (iPPVO) is currently used in equine medicine as an immunomodulator to improve the immune system and as a prophylactic treatment to prevent or treat infectious diseases. This study was designed to determine the effects of iPPVO on polymorphonuclear leukocyte (PMNL) function (phagocytosis and intracellular killing activity) and the myeloperoxidase (MPO) activity of PMNLs in horses. Twenty-four healthy English thoroughbred horses with an average age of 11 years were included in the study. Venous blood samples (10 ml) were taken before (agentfree controls) and after the administration of iPPVO (2 ml i.m. injection on Days 1, 3, and 5). PMNLs (1 × 10 7 cells/ml) were isolated from venous blood containing EDTA (0.1 g/ml) with Ficoll-Hypaque gradient centrifugation. Cellular phagocytosis and intracellular killing activities were assayed using a modification of Alexander's method before and after treatment with iPPVO. MPO activity was also measured. The administration of iPPVO significantly increased the phagocytic, intracellular killing, and MPO activities of equine PMNLs (P = 0.0058, P = 0.0050, and P = 0.0070, respectively). This study demonstrates a strong correlation between MPO activity and PMNL function. The administration of iPPVO to horses has a supportive effect on their cellular immunity and an immunomodulatory effect against equine viral infections.

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“…Als oxidativer Burst wird die Produktion von bakteriziden reaktiven Sauerstoffradikalen durch Monozyten/Makrophagen und neutrophile Granulozyten bezeichnet (30). In In-vitro-Studien ließ sich die Induktion dieser frühen Mechanismen in kaninen Leukozyten nachweisen (32,33). Nach Stimulation durch PIND-ORF wurde mittels Durchflusszytometrie eine Erhöhung der Phagozytoserate von kaninen Monozyten und neutrophilen Granulozyten festgestellt.…”

Section: Wirkungsweiseunclassified

Einsatz von Paramunitätsinducern in der Kleintiermedizin

Proksch¹,

Hartmann

2016

Tierarztl Prax Ausg K

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Paramunity inducers have been used to treat small animals for decades. Paramunity inducers are based on attenuated and inactivated poxviruses (avipox virus and parapox virus). Their applications include both therapeutic and prophylactic use in various diseases. Despite their wide and variable use, only a very small number of placebo-controlled studies has been published. Positive effects in preventing kitten mortality and in treating feline stomatitis have been reported, however, no statistically significant effect of their therapeutic use in canine parvovirus infection, feline leukemia infection virus infection or canine papillomavirus infection could be demonstrated. For these infectious diseases, paramunity inducers do not appear to be effective.

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Specific antibodies induced by inactivated parapoxvirus ovis potently enhance oxidative burst in canine blood polymorphonuclear leukocytes and monocytes

Cited by 14 publications

References 31 publications

Inactivated Parapoxvirus ovis induces a transient increase in the expression of proinflammatory, Th1-related, and autoregulatory cytokines in mice

Inactivated Parapoxvirus ovis induces a transient increase in the expression of proinflammatory, Th1-related, and autoregulatory cytokines in mice

Effects of inactivated Parapoxvirus ovis on polymorphonuclear leukocyte function and myeloperoxidase activity in horses

Einsatz von Paramunitätsinducern in der Kleintiermedizin

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