Inability of Monoclonal Anti‐light Chain Antibody to Detect Clonal B‐Cells in a Patient with Follicular Lymphoma by Multicolor Flow Cytometry

Velzen, Jeroen F. van; Blink, Dorine van den; Wiegers, Ingrid E.H.; Bloem, Andries C.

doi:10.1002/jcla.21716

Cited by 1 publication

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“…If surface light chains are not detected with polyclonal antibody reagents, cytoplasmic light chain study is quite useful to further assess the light chain expression patterns. Our observation is consistent with published literature indicating that polyclonal antibodies are more likely to detect light chain expression than monoclonal antibodies (Horna et al, 2011; van Velzen et al, 2014).…”

Section: Discussionsupporting

confidence: 93%

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The patterns and diagnostic significance of the lack of surface immunoglobulin light chain on mature B cells in clinical samples for lymphoma workup

Huang

Fennell

Chen

et al. 2022

Cytometry Part B Clinical

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Background Surface immunoglobulin (sIg) light chains are not always detected on mature B cells. This may present as a challenge for clonality determination in clinical flow cytometry. Methods To explore the mechanism and diagnostic significance of sIg negative mature B cells, we retrospectively studied 14 cases of sIg negative reactive B‐cell lymphocytosis and 89 cases of sIg negative mature B‐cell lymphomas. The expression patterns of sIg and cytoplasmic immunoglobulin (cIg) light chains were studied by flow cytometry using both monoclonal and polyclonal antibodies. Results These 14 cases of sIg negative reactive B‐cell lymphocytosis were proven to be polytypic based on cytoplasmic light chain studies. In 89 cases of sIg negative mature B‐cell lymphomas, we described four distinct patterns of abnormal light chain expression including partial or complete loss of sIg or cIg, suggesting different underlying mechanisms. Conclusions This study represents the first reported series of body or cystic fluids where reactive B cells do not have detectable sIg, arguing strongly against making a diagnosis of B‐cell lymphoma based on lack of sIg in mature B cells. Since the lack of sIg does not always predict clonal/neoplastic mature B‐cell proliferation, further cIg evaluation should be performed when sIg expression is not detected in mature B cells. The lack of both sIg and cIg in mature B cells may serve as a reliable surrogate clonality/neoplastic marker.

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Section: Discussionsupporting

confidence: 93%

“…By contrast, B cells showed a polytypic pattern of cytoplasmic light chains were identified as nongerminal center B cells (CD20+, CD10À, gray, Figure 4c). light chain expression than monoclonal antibodies (Horna et al, 2011;van Velzen et al, 2014).…”

Section: Surface Light Chain Negative Mature Neoplastic B Cellsmentioning

confidence: 92%

The patterns and diagnostic significance of the lack of surface immunoglobulin light chain on mature B cells in clinical samples for lymphoma workup

Huang

Fennell

Chen

et al. 2022

Cytometry Part B Clinical

View full text Add to dashboard Cite

show abstract

Inability of Monoclonal Anti‐light Chain Antibody to Detect Clonal B‐Cells in a Patient with Follicular Lymphoma by Multicolor Flow Cytometry

Abstract: These data illustrate when antibodies against SIg light chains are employed for B-cell clonality assessment, polyclonal antibodies are preferred over monoclonal antibodies.

Cited by 1 publication

References 2 publications

The patterns and diagnostic significance of the lack of surface immunoglobulin light chain on mature B cells in clinical samples for lymphoma workup

The patterns and diagnostic significance of the lack of surface immunoglobulin light chain on mature B cells in clinical samples for lymphoma workup

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