In vivo visualization of hemodialysis-induced alterations in leukocyte–endothelial interactions

Teijlingen, Marco E. van; Borgdorff, Piet; Wijhe, Michiel H. van; Lambalgen, Ton A. van; Wee, Piet M. ter; Tangelder, G. J.

doi:10.1046/j.1523-1755.2000.00121.x

Cited by 8 publications

(11 citation statements)

References 39 publications

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“…On the other hand, rabbits transport most of the cholesterol in LDL, as do humans, making it a more suitable model for cardiovascular research [14]. Furthermore, the rabbit has a larger blood volume (∼56 mL/kg BW) [15,] thereby making it more suitable for repeated blood sampling and research on extracorporeal devices (e.g., hemodialysis or peritoneal dialysis) [16,17]. To the best of our knowledge, only a limited number of studies implemented 5/6 subtotal nephrectomy to induce CKD in rabbits [18,19,20,21,22,23,24,25,26,27].…”

Section: Introductionmentioning

confidence: 99%

Adenine Rich Diet Is Not a Surrogate of 5/6 Nephrectomy in Rabbits

Florens

Lemoine

Pelletier

et al. 2017

Nephron

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Background: Animal models are important tools needed to understand the mechanisms underlying the progression of renal disease and to implement new therapeutic approaches. A non-surgical model of chronic kidney disease (CKD) developed by chemical nephrectomy using an adenine-enriched diet has been shown to be a robust model to induce kidney failure in mice and rats. The purpose of this study was to implement an adenine diet to induce CKD in rabbits. Methods: Male New Zealand rabbits were fed for 4 weeks with a diet containing 0.75% (w/w) adenine, and renal function was assessed by measuring plasma urea and creatinine concentrations. The glomerular filtration rate (GFR) was measured using the plasmatic clearance of Iohexol. Kidney histology was performed with haematoxylin erythrosine saffron and Sirius red staining. Results: In contrast to what was observed in rodents, adenine diet failed to induce kidney failure in rabbits as is evident in the plasma concentrations of creatinine and urea and the direct measurement of GFR or histopathological studies. Conclusion: Adenine diet is not a surrogate of subtotal nephrectomy to induce kidney failure in rabbits. Several interspecies differences in metabolism and renal physiology could account for this observation.

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Section: Introductionmentioning

confidence: 99%

Adenine Rich Diet Is Not a Surrogate of 5/6 Nephrectomy in Rabbits

Florens

Lemoine

Pelletier

et al. 2017

Nephron

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“…Activation of these systems in turn activates cellular defense mechanisms 5. Many cell types are activated; some of the most important ones are neutrophils and platelets 3, 6. During cardiopulmonary bypass there is a systemic inflammatory response that in part is caused by the biomaterial contact.…”

Section: Introductionmentioning

confidence: 99%

Optimal heparin surface concentration and antithrombin binding capacity as evaluated with human non‐anticoagulated blood in vitro

Andersson

Sánchez

Ekdahl

et al. 2003

J Biomedical Materials Res

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Contact between blood and a biomaterial surface takes place in many applications and is known to activate the coagulation and complement systems. Heparin surface coatings have been shown to reduce blood activation upon contact with artificial surfaces. To establish the optimal heparin surface concentration, blood was incubated in a tubing loop model at 37 degrees C. The tubing was coated with different surface concentrations of heparin and rotated at three different velocities. We demonstrate that the blood compatibility of a surface with regard to coagulation, complement, and platelet activation can be improved by increasing the heparin surface concentration in the 6-12 pmol antithrombin/cm2 concentration interval. The binding of factor H is not influenced by the increased heparin surface concentration, suggesting that this factor is not the primary regulator of complement on heparin surfaces. In addition, the heparin coating has no effect on the complement activation that occurs on gas surfaces in extracorporeal circuits.

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“…In fact, the quantitative expression of adhesion molecules on inflammatory cells does not always mirror the functional state of the cell [1]. Previous studies performed on endothelial cells in culture and in experimental animals suggest that dialysis with cuprophan membranes results in diminished leukocyte rolling and impaired endothelial adhesion [9, 11, 12, 13]. However, whether this also results in impaired transmigration properties is not known.…”

Section: Introductionmentioning

confidence: 99%

Impact of Hemodialysis Membrane and Permeability on Neutrophil Transmigration in vitro

Moshfegh¹,

Jacobson²,

Halldén³

et al. 2002

Nephron

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Background/Aim: The impact of dialysis membrane permeability on neutrophil transmigration properties in vitro was examined in the present study. This issue has not been fully scrutinized before. Methods: We studied the capacity of neutrophils collected from a group of dialysis patients randomly treated with cuprophan, low- or high-flux polysulfone, to transmigrate in vitro through a membrane covered with fibronectin (a main constituent of the endothelial basement membrane). The hemodialysis-induced quantitative changes in expression of adhesion molecules were examined in parallel. Results: At the end of dialysis, neutrophils collected from patients treated with high-flux polysulfone dialyzers had a significantly higher transmigration index than neutrophils from patients treated with low-flux polysulfone membrane (p < 0.01) or cuprophan membrane (p < 0.01), and approached the level of transmigration observed in neutrophils collected from healthy controls. In the groups treated with low-flux polysulfone and cuprophan dialyzers, the transmigration capacity was significantly lower (p < 0.02) compared to neutrophils from healthy subjects. We also noted that differences between low- and high-flux polysulfone dialysis, in the context of transmigration properties, were not mirrored by changes in adhesion phenotype, which strengthens the view that there is no strict relationship between these two features. Conclusion: The study demonstrates that high-flux polysulfone dialysis, as opposed to low-flux polysulfone and cuprophan treatment, improves the transmigration properties of circulating neutrophils, despite similar effects on adhesion molecule phenotypes. A plausible mechanism is that potentially toxic middle range molecules that inhibit neutrophil migration are more efficiently eliminated during high-flux polysulfone dialysis, but this explanation requires further support.

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In vivo visualization of hemodialysis-induced alterations in leukocyte–endothelial interactions

Cited by 8 publications

References 39 publications

Adenine Rich Diet Is Not a Surrogate of 5/6 Nephrectomy in Rabbits

Adenine Rich Diet Is Not a Surrogate of 5/6 Nephrectomy in Rabbits

Optimal heparin surface concentration and antithrombin binding capacity as evaluated with human non‐anticoagulated blood in vitro

Impact of Hemodialysis Membrane and Permeability on Neutrophil Transmigration in vitro

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