In vivo temporal evolution of ALA-induced normalized fluorescence at different anatomical locations of oral cavity: Application to improve cancer diagnostic contrast and potential

Mallia, Rupananda J.; Subhash, Narayanan; Stolzmann, Paul; Kumar, Rejnish; Thomas, Shiny Sara; Mathews, Anitha; Madhavan, Jayaprakash

doi:10.1016/j.pdpdt.2010.06.006

Cited by 12 publications

(13 citation statements)

References 41 publications

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“…The main clinical application of ALA-PDT has been in the treatment of nonmelanoma skin lesions, mainly for basal cell carcinoma using topical application, although treatment of highgrade dysplasia in Barrett's esophagus, cervical neoplasia, and cancer in the oral cavity, has also been investigated (75)(76)(77). ALA administration has also been applied in the fluorescence-guided resection of bladder cancer, and intraepithelial lesions of the cervix, lung, and brain cancer (6)(7)(8)(9)(10).…”

Section: Discussionmentioning

confidence: 99%

The Use of Dipeptide Derivatives of 5-Aminolaevulinic Acid Promotes Their Entry to Tumor Cells and Improves Tumor Selectivity of Photodynamic Therapy

Venosa

Vallecorsa

Giuntini

et al. 2015

Molecular Cancer Therapeutics

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The use of endogenous protoporphyrin IX generated after administration of 5-aminolaevulinic acid (ALA) has led to many applications in photodynamic therapy (PDT). However, the bioavailability of ALA is limited by its hydrophilic properties and limited cell uptake. A promising approach to optimize the efficacy of ALA-PDT is to deliver ALA in the form of prodrugs to mask its hydrophilic nature. The aim of this work was to evaluate the potential of two ALA dipeptide derivatives, N-acetyl terminated leucinyl-ALA methyl ester (Ac-Leu-ALA-Me) and phenylalanyl-ALA methyl ester (Ac-Phe-ALA-Me), for their use in PDT of cancer, by investigating the generation of protoporphyrin IX in an oncogenic cell line (PAM212-Ras), and in a subcutaneous tumor model. In our in vitro studies, both derivatives were more effective than ALA in PDT treatment, at inducing the same protoporphyrin IX levels but at 50-to 100-fold lower concentrations, with the phenylalanyl derivative being the most effective. The efficient release of ALA from Ac-Phe-ALA-Me appears to be consistent with the reported substrate and inhibitor preferences of acylpeptide hydrolase. In vivo studies revealed that topical application of the peptide prodrug Ac-Phe-ALA-Me gave greater selectivity than with ALA itself, and induced tumor photodamage, whereas systemic administration improved ALA-induced porphyrin generation in terms of equivalent doses administered, without induction of toxic effects. Our data support the possibility of using particularly Ac-Phe-ALA-Me both for topical treatment of basal cell carcinomas and for systemic administration. Further chemical fine-tuning of this prodrug template should yield additional compounds for enhanced ALA-PDT with potential for translation to the clinic. Mol Cancer Ther; 14(2); 440-51. Ó2014 AACR.

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Section: Discussionmentioning

confidence: 99%

The Use of Dipeptide Derivatives of 5-Aminolaevulinic Acid Promotes Their Entry to Tumor Cells and Improves Tumor Selectivity of Photodynamic Therapy

Venosa

Vallecorsa

Giuntini

et al. 2015

Molecular Cancer Therapeutics

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“…The collected fluorescence spectra differ depending on the excitation wavelength, oral site, biochemical composition and tissue architecture [28,29]. Distinct excitation wavelengths present different photon energies, resulting in distinct light-tissue interaction, and may excite different endogenous fluorophores [26,30].…”

Section: Discussionmentioning

confidence: 99%

Fluorescence spectroscopy for the detection of potentially malignant disorders and squamous cell carcinoma of the oral cavity

Francisco

Correr

Azevedo

et al. 2014

Photodiagnosis and Photodynamic Therapy

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“…Based on the ratio between these, a sensitivity of 82% and specificity of 100% were reported . Various other studies have provided further evidence in support of in vivo fluorescence spectroscopy for noninvasive oral cancer diagnosis (Supplementary reference 6 and 7) . A recent study by Nazeer Shaiju et al showed that autofluorescence spectroscopy can be used to identify oral cavity disorders caused by long‐term tobacco habits.…”

Section: Introductionmentioning

confidence: 95%

Recent advances in optical diagnosis of oral cancers: Review and future perspectives

Singh

Ibrahim

Byrne³

et al. 2015

Head & Neck

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Optical diagnosis techniques offer several advantages over traditional approaches, including objectivity, speed, and cost, and these label-free, noninvasive methods have the potential to change the future workflow of cancer management. The oral cavity is particularly accessible and, thus, such methods may serve as alternate/adjunct tools to traditional methods. Recently, in vivo human clinical studies have been initiated with a view to clinical translation of such technologies. A comprehensive review of optical methods in oral cancer diagnosis is presented. After an introduction to the epidemiology and etiological factors associated with oral cancers currently used, diagnostic methods and their limitations are presented. A thorough review of fluorescence, infrared absorption, and Raman spectroscopic methods in oral cancer diagnosis is presented. The applicability of minimally invasive methods based on serum/saliva is also discussed. The review concludes with a discussion on future demands and scope of developments from a clinical point of view. © 2015 Wiley Periodicals, Inc. Head Neck 38: E2403-E2411, 2016.

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In vivo temporal evolution of ALA-induced normalized fluorescence at different anatomical locations of oral cavity: Application to improve cancer diagnostic contrast and potential

Cited by 12 publications

References 41 publications

The Use of Dipeptide Derivatives of 5-Aminolaevulinic Acid Promotes Their Entry to Tumor Cells and Improves Tumor Selectivity of Photodynamic Therapy

The Use of Dipeptide Derivatives of 5-Aminolaevulinic Acid Promotes Their Entry to Tumor Cells and Improves Tumor Selectivity of Photodynamic Therapy

Fluorescence spectroscopy for the detection of potentially malignant disorders and squamous cell carcinoma of the oral cavity

Recent advances in optical diagnosis of oral cancers: Review and future perspectives

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