In vivo study on metal release from fixed orthodontic appliances and DNA damage in oral mucosa cells

Faccioni, Fiorenzo; Franceschetti, Paola; Cerpelloni, Marzia; Fracasso, Maria Enrica

doi:10.1016/j.ajodo.2003.09.010

Cited by 225 publications

(194 citation statements)

References 21 publications

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“…Faccioni et al presented 3 forms damaging the DNA structure (comets, apoptosis and viability), whereas other authors presented one in different units. 17,[19][20][21][22][23] The results presented by Angelieri et al and Heravi et al showed that the micronucleus frequencies were not significantly different before, during and after orthodontic treatment, and are in contrast with those of Natarajan et al, who found a significantly higher MN count in the test group at the day of debonding as compared to the control group without appliances. 20,22,23 Hafez et al 19 found that the cytotoxicity and genotoxicity of orthodontic appliances remained in the mouth for 6 months.…”

Section: Discussioncontrasting

confidence: 53%

“…Faccioni et al and Amini et alreport a double or triple increase in the level of nickel and cobalt ions in the cells of cheek mucosa epithelium. 17,18 Statistically significant differences were observed for cobalt (Co) 17 , nickel (Ni) 17,18 and chromium (Cr) 19 . In another study, the differences between cobalt and chromium were not statistically significant, although the p-level was low (0.09 and 0.10).…”

Section: Discussionmentioning

confidence: 99%

“…17 The fixed orthodontic appliances were in the upper and lower jaw (20 brackets, 4-8 bands each). The metal ions were released during the first 4 or 5 months of orthodontic therapy.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Trace metal ions release from fixed orthodontic appliances and DNA damage in oral mucosa cells by in vivo studies: A literature review

Downarowicz¹,

Mikulewicz²

2017

Adv Clin Exp Med

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An overview of professional literature referring to the release of metal ions from fixed orthodontic appliances and their influence on oral mucosa in conditions of in vivo are presented, along with a detailed analysis of the exposure of the cells of cheek mucosa epithelium to metal ions. Electronic databases (PubMed, Elsevier, Ebsco) were searched with no language restrictions. The relevant orthodontic journals and reference lists were checked for all eligible studies. A total of 38 scientific articles were retrieved in the initial search. However, only 7 articles met the inclusion criteria. Statistically significant differences in the levels of the amount of nickel ions, cobalt ions and chromium ions were observed in cells of cheek mucosa. The most biocompatible material used in the production of fixed orthodontic appliances is titanium, and the least biocompatible material is steel, which releases the largest amount of nickel and chromium. Metal ions are released from fixed orthodontic appliances only in the first phase of treatment. It is recommended to conduct further, long-term research on a larger number of patients to define the influence of using fixed orthodontic appliances and biological effect they might have on tissues.

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Section: Discussioncontrasting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

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Trace metal ions release from fixed orthodontic appliances and DNA damage in oral mucosa cells by in vivo studies: A literature review

Downarowicz¹,

Mikulewicz²

2017

Adv Clin Exp Med

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“…[28][29][30][31][32] In this study, buccal mucosa cells were evaluated only 30 days after removal of the brackets, because Jones et al 22 recommend that if a lesion persists for longer than 14 days after removal of the causative factors, a biopsy should be performed immediately. Therefore, within 30 days, cells should have returned to their initial size.…”

Section: Discussionmentioning

confidence: 99%

Metal and Ceramic Bracket Effects on Human Buccal Mucosa Epithelial Cells

Pereira

Tanaka

Lima

et al. 2009

The Angle Orthodontist

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Objective: To test the null hypothesis that metal and ceramic brackets have no effect on the epithelial cells of the buccal mucosa. Materials and Methods: Two metal and two ceramic brackets were bonded in 21 individuals of both sexes. With the use of liquid-based exfoliative cytology, morphometric and morphologic changes in buccal mucosa cells adjacent to these brackets were determined and were compared at three time points: baseline (T0), 60 days after placement (T1), and 30 days after removal of the brackets (T2). Results: A decrease in nuclear area and an increase in cytoplasmic area occurred in the buccal mucosa cells adjacent to the brackets at T1 (P Ͻ .01). At T2, this altered morphometry persisted only in cells adjacent to the metal brackets, although to a lesser degree than at T1 (P Ͻ .01). A greater decrease in nuclear area was noted in cells adjacent to the metal brackets than in those next to the ceramic brackets (P Ͻ .01). At T0, the proportions of surface and subsurface cells were similar, but at T1, a predominance of surface cells was observed (P Ͻ .05). At all time points, smears of cells appeared normal or normal with some inflammatory changes. Conclusion:The hypothesis is rejected. Placement of metal and ceramic brackets in the buccal cavity induces cellular alterations. These alterations do not suggest malignancy. (Angle Orthod. 2008;00:373-379.)

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“…When viewed under a microscope, a cell has the appearance of a comet, with head (the nuclear region) and tail containing DNA fragments or strands migrating in the direction of the anode. In particular, this assay seems important for the investigation of suspected genotoxins in vitro 21 and in vivo 5 . As a result and because of inappropriate evidence, the purpose of this study was to investigate, by the single cell gel (comet) assay in vitro, whether sodium fluoride (NaF) induces DNA breakage in Chinese hamster ovary (CHO) cells or not.…”

Section: Introductionmentioning

confidence: 99%

Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro

et al. 2004

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Fluoride has been widely used in dentistry because it is a specific and effective caries prophylactic agent. However, excess fluoride may represent a hazard to human health, especially by causing injury to genetic material. Genotoxicity tests represent an important part of cancer research to assess the risk of potential carcinogens. In the current study, the potential DNA damage associated with exposure to fluoride was assessed by the single cell gel (comet) assay in vitro. Chinese hamster ovary cells were exposed to sodium fluoride (NaF) at final concentration ranging from 7 to 100 µg/ml for 3 h, at 37°C. The results pointed out that NaF in all concentrations tested did not contribute to DNA damage as depicted by the mean tail moment and tail intensity. These findings are clinically important since they represent an important contribution to a correct evaluation of the potential health risk associated with the exposure to dental agents. DESCRIPTORS: Sodium fluoride; Comet assay; Mutagenicity tests. RESUMO:O flúor tem sido amplamente usado na Odontologia, pois é um agente profilático efetivo e específico contra a cárie dentária. Entretanto, o flúor em excesso pode representar perigos à saúde humana, especialmente por causar agressão ao material genético. Testes de genotoxicidade representam uma importante parte da pesquisa do câncer para a avaliação de risco de possíveis carcinógenos. Neste presente estudo, danos ao DNA associados à exposição ao flúor foram avaliados pelo teste de células individualizadas em gel de agarose (teste do cometa) in vitro. Células de ovário de hamster chinês foram expostas ao fluoreto de sódio (NaF) nas concentrações finais de 7 a 100 µg/ml, durante 3 h, a 37°C. Os resultados mostraram que o NaF não contribuiu para os danos no DNA em todas as concentrações testadas, conforme demonstrado pelas médias do momento da cauda e da intensidade da cauda dos cometas. Esses achados são clinicamente importantes, uma vez que representam uma importante contribuição para a correta avaliação do potencial risco à saúde associada à exposição aos agentes odontológicos. DESCRITORES: Fluoreto de sódio; Ensaio em cometa; Testes de mutagenicidade.

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In vivo study on metal release from fixed orthodontic appliances and DNA damage in oral mucosa cells

Cited by 225 publications

References 21 publications

Trace metal ions release from fixed orthodontic appliances and DNA damage in oral mucosa cells by in vivo studies: A literature review

Trace metal ions release from fixed orthodontic appliances and DNA damage in oral mucosa cells by in vivo studies: A literature review

Metal and Ceramic Bracket Effects on Human Buccal Mucosa Epithelial Cells

Fluoride does not induce DNA breakage in Chinese hamster ovary cells in vitro

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