In Vivo Study of the Action of a Topical Anti-Inflammatory Drug In Rat Teeth Submitted To Dental Bleaching

Abstract

Bleaching gel containing hydrogen peroxide (H2O2) cause damages in pulp tissue. This study investigated the action of a topical anti-inflammatory, the Otosporin®, in rats’ bleached teeth with the null hypothesis of which the Otosporin® is no able to minimize the pulp inflammation that bleaching gel generates. The rat’s molars were divided into groups: BLE: bleached (35% H2O2 concentration /single application of 30 min); BLE-O: bleached followed by Otosporin® (10 min); and control: placebo gel. In the second day after dental bleaching, the rats were killed, and the jaws were processed for hematoxylin-eosin and immunohistochemistry analysis for tumor necrosis factor alpha (TNF-α), interleukin (IL)-6 and IL-17. The data collected were subjected to Kruskal-Wallis and Dunn statistical tests with at a 5% level of significance (p<0.05). The BLE group had moderate to strong inflammation in the occlusal third of the coronary pulp, with necrotic areas; and BLE-O, mild inflammation (p<0.05). There was a significant difference in the occlusal and middle thirds of the coronary pulp between the BLE with BLE-O and control groups (p<0.05). There was no difference in the cervical third (p>0.05). The BLE group had a high immunoexpression of TNF-α than BLE-O and control groups (p<0.05), with moderate and mild immunoexpression, respectively. Regarding IL-6 and IL-17, the BLE group had higher immunoexpression than control (p<0.05); the BLE-O was similar to the control (p>0.05). The topical anti-inflammatory Otosporin® can reduce pulp inflammation after dental bleaching in the rat teeth.

Effect of dental bleaching on the microhardness and surface roughness of sealed composite resins

“…The process of dental bleaching has been a subject of discussion in dentistry because it is a conservative technique that is effective for whitening natural teeth [1][2][3], but conflicting results have been documented regarding its effects on restorative materials [4]. The bleaching agents in current use are peroxides in gel form, with concentrations ranging from 4% to 22% and from 25% to 40% for at-home and in-office bleaching techniques, respectively [5,6].…”

TRPA1 triggers hyperalgesia and inflammation after tooth bleaching

“…As mentioned above, ATP acts as a neurotransmitter to stimulate neurons and produce pain. Tooth bleaching produced inflammation, causing pulpitis in some teeth 29 . Based on the mechanism underlying inflammation, the inflammatory response and complications of pulpitis can be attenuated.…”

Influence of supplement administration of omega‐3 on the subcutaneous tissue response of endodontic sealers in Wistar rats

“…To evaluate the inflammatory infiltrate that was graded in scores from 1 to 4 (1 – no or few inflammatory cells and no reaction; 2 – less than 25 cells and little reaction; 3 – between 25 and 125 cells and moderate reaction; 4 – 125 or more cells and severe reaction). The scores for the immunolabelling of IL‐6 and TNF‐α were graded also from 1 to 4 (1 – absence of immunostaining in the region of interest; 2 – low immunolabelling pattern, about one‐fourth of labelling of cells and poor labelling in extracellular matrix; 3 – moderate immunolabelling pattern, about half of the immunolabelled cells and moderate labelling in the extracellular matrix and 4 – high immunolabelling pattern, about three‐fourths of immunolabelled cells and high labelling in the extracellular matrix; Benetti, Briso, et al, 2018a).…”