Influence of supplement administration of omega‐3 on the subcutaneous tissue response of endodontic sealers in Wistar rats

Abstract

Aim Natural substances such as omega‐3 have been used in the medical field due to their numerous properties and, in particular, modulating effect on the systemic and local inflammatory processes. Thus, this study evaluated the influence of omega‐3 supplementation on the subcutaneous tissue response of endodontic sealers in Wistar Rats. Methodology Polyethylene tubes were implanted in the subcutaneous tissue of 48 animals (one empty for control and three filled with Sealapex, AH Plus or Endofill). The animals were treated with omega‐3 (TO) or water (TW). Treatments started 15 days before implantation until euthanasia. After 5, 15 and 30 days (n = 8), animals were euthanized and polyethylene tubes and surrounding tissue were removed and processed for histological analysis. The inflammatory reaction was analysed by Haematoxylin and Eosin stain and immunolabelling for IL‐6 and TNF‐α. The collagen maturity was analysed by picrosirius red stain and calcium deposition by von Kossa stain and polarized light. Results were statistically analysed (p < .05). Results Amongst TW sealer groups, Endofill evoked a more intense inflammatory infiltrate compared with AH Plus and control in the 30‐day period (p = .009). However, in TO sealer groups, there was no difference amongst the sealers and control in all periods (p > .05). Comparing each sealer as a function of the supplementation with water or omega‐3, there are differences for Endofill (p = .001) and Sealapex (p = .005) in the 30‐day period, presenting lower inflammatory infiltrate in the animals treated with omega‐3. A higher percentage of immature fibres was observed at 15 and 30 days in the TO group, compared with the TW group (p < .05). The deposition of calcium particles was observed only by Sealapex in all periods, despite the supplementation procedure. Conclusions Omega‐3 supplementation influence the tissue reactions of endodontic sealers, modulating inflammation, the immunolabelling of IL‐6 and TNF‐α, the repair process and it does not interfere with calcium deposition.

Cytotoxicity, biocompatibility and calcium deposition capacity of 45S5 bioglass experimental paste and Bio-C Temp: in vitro and in vivo study using Wistar rats

“…The number of animals was established following the protocols of previous studies, using an alpha error of 0.05% and 95% power to recognize a signi cant difference of 1 in the median scores, a minimum of seven animals per group was considered necessary. Considering that complications could occur during the study, one more animal was added for each period of analysis (7 and 30 days) [20,23].…”

Influence of melatonin supplementation on tissue response of endodontic sealers in Wistar rats

“…Considering three observation times, 48 animals were necessary in this study [4,27,29], They were caged in a controlled temperature and light cycle environment (22 ± 1 ° C, 70% humidity at 12 hours light and 12 hours dark), received solid diet and ad libitum water, except for the rst 24 hours after the intervention. The experimental procedures were approved and performed according to the guidelines of the ethical committee (process no.…”

“…The implanted tubes were removed with the surrounding tissue and xed in 1% formalin solution at a pH of 7.0. The specimens were imbibed in para n and sectioned into 5-μm cuts for staining with hematoxylin-eosin, picrosirius red (PSR), immunohistochemistry for IL-6 and TNF-α and 10-μm cuts for staining with von Kossa and not stained for polarized light technique [27,34].…”

“…Considering that endodontic sealers can develop local in ammatory reactions on connective tissues and that melatonin is a potential regulator of the in ammatory process, the present study evaluated the in uence of melatonin supplementation on tissue's response to endodontic sealers by a consolidated method of exploring the biomaterials response, the implantation of polyethylene tubes in subcutaneous tissues of Wistar rats [27]. Null hypothesis: Melatonin would not interfere with the tissue's response to endodontic sealers.…”