In vivo study of the effectiveness of ozonized olive oil gel on inhibiting enamel demineralization during orthodontic treatment

Abstract

The present study assessed the effect of ozonized olive oil gel during orthodontic treatment to inhibit demineralization around orthodontic brackets.Material and Methods: Material and Methods: Material and Methods: Material and Methods: Fourteen patient, aged 15-18 years, scheduled to have premolars extraction as a part of orthodontic treatment participated in the present study. The patients were randomly divided into 3 groups; control group (16 upper and lower premolars) and two experimental groups (20 upper and lower premolars for each). In the Control group no treatment was applied on the premolars. In experimental groups, after etching and bonding, T-loops were formed with 0.014 inch stainless steel wire and engaged on the experimental teeth. In experimental group II, ozonized olive oil gel was applied sparingly to the tooth surface with concentration of 20:25 µgm/ml. Participants were instructed to apply the ozonized olive oil gel to the buccal surface of the premolars three times daily for 8 weeks after regular oral hygiene procedures. After 8 weeks, the brackets were deboned and the premolars extracted carefully. The premolars of each group were divided into 2 equal numbers; 28 premolars prepared for elemental analyses (calcium and phosphorus) followed by scanning electron microscope investigation, and 28 premolars examined with polarized light. ANOVA test was applied followed by post hoc LSD test to compare between each 2 groups.

Better With Ozone, or Not? An in-Vivo Study of Ozone Therapy as a Pre-Treatment Before Fissure Sealant Application.

“…According to the study report, ozone application improved remineralization for all groups; however, based on DIAGNOdent scores, the increases in remineralization observed with Fuji Triage and Aegis FS following ozone treatment were significantly higher (p<0.05) than those observed with Helioseal following ozone treatment. 15 These findings were similar with the recent study, though statistically significant results were not detected in.…”

“…[10][11][12] A number of in-vivo studies have also been conducted to investigate the antimicrobial effects of ozone therapy and the effects of pre-treatment with ozone gas on the success of resin-based materials. [13][14][15][16][17] Clinpro™ is a resin-based fluoride-releasing sealant that includes inorganic filler components that is frequently used in clinical practice and that has been featured in many recent studies. 18 Teethmate F-1 is an unfilled, low-viscosity, resinbased material capable of diffusing through deep pits and fissures.…”

Ozonated Oils as Antimicrobial Systems in Topical Applications. Their Characterization, Current Applications, and Advances in Improved Delivery Techniques

“…Moreover, a gel preparation of ozonated olive oil has been added to a standard oral hygiene regimen and was found to reduce enamel demineralization around orthodontic brackets during orthodontic treatment [69]. Another gel preparation, O-zone gel, was compared with two chlorhexidine digluconate (CHX) based agents.…”

Ozone Therapy - A Boon to Dental Sciences

“…So, proper enamel prophylaxis is required in orthodontics, prior to bracket placement. Ghobashy et al 32 evalauted the effects of ozonized olive oil gel in reducing enamel demineralization around orthodontic brackets. The use of ozonized olive oil gel along with standard prophylaxis showed significantly less decalcification of teeth among orthodontics patients.…”