In vivo study of hot compressing molded 50:50 poly (DL‐lactide‐co‐glycolide) antibiotic beads in rabbits

Ueng, Steve Wen–Neng; Yuan, Li-Jen; Liao, Ning; Lin, Song–Shu; Liu, Shih‐Jung; Chan, Err–Cheng; Weng, Jui-Hsun

doi:10.1016/s0736-0266(01)00174-7

Cited by 26 publications

(19 citation statements)

References 37 publications

(45 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, there has not been an animal model specific for detecting the sequential changes in local antibiotic concentration. The animal model used in this study was designed by the authors specifically for the investigation of local antibiotic release profiles from antibiotic carriers [45]. In the rabbit model, a granulated bony cavity at the distal femur was created for the implantation of antibiotic beads.…”

Section: Discussionmentioning

confidence: 99%

“…I). Anirntrl mode/ A rabbit animal model was specifically designed by the authors for the investigation of local antibiotic release profiles from antibiotic carriers [45]. In this model, a bony cavity was surgically made at left and right distal femur of each animal.…”

Section: Prepcirution Of Untihiotic Hcritfsmentioning

confidence: 99%

“…Alginate is one of the most promising biodegradable materials [3,7,8,12,13,40,43,45,46]. It is a product from marine algae and is a linear polyuronate, copolymer of D-mannuronic acid and L-gulonic acid.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vivo study of biodegradable alginate antibiotic beads in rabbits

Ueng

Yuan

Liao

et al. 2004

Journal Orthopaedic Research

Self Cite

View full text Add to dashboard Cite

The authors investigated the lyophilized poly-L-lysine-coated alginate antibiotic delivery system in vivo for the treatment of musculoskeletal infections. The sodium alginate was mixed with vancomycin, coated with poly-L-lysine and lyophilized to form 3 mm in diameter biodegradable antibiotic beads. The antibiotic beads were implanted in the distal femoral cavities of rabbits for in vivo investigation. The local concentration of vancomycin was well above the minimal inhibitory Concentration of Stuph~d~vloco~us uureus for 21 days. The release was most marked during the first two days. The diameters of sample inhibition zone ranged from 8 to 16 mm, the relative activity of vancomycin ranged from 12.5'%1 to 100'%1. The blood level of vancomycin reached its peak (46.0 mgll) two days after implantation and fell to 3.2 mgll at two weeks. It was undetectable after three weeks. There was no increase in the concentration of blood urea nitrogen and serum creatiiiine after the implantation. Histological observations showed that bead materials were biodegradable, resorbed slowly and only cause mild host reaction. This study offers a biodegradable delivery system of antibiotics to treat musculoskeletal infections.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Prepcirution Of Untihiotic Hcritfsmentioning

confidence: 99%

See 1 more Smart Citation

In vivo study of biodegradable alginate antibiotic beads in rabbits

Ueng

Yuan

Liao

et al. 2004

Journal Orthopaedic Research

Self Cite

View full text Add to dashboard Cite

show abstract

“…[7][8][9][10][11][12][13][14][15] Alginate, a very promising biodegradable material, [16][17][18][19] has been utilized as a support matrix for entrapping and culturing malignant cells and chondrocytes. Furthermore, previous in vitro studies have identified a stabilizing effect of alginate beads on hydrophilic b-lactam antibiotics and epithelial proliferation growth factors.…”

Section: Introductionmentioning

confidence: 99%

Development of a biodegradable alginate carrier system for antibiotics and bone cells

Ueng

Lee

Lin

et al. 2006

Journal Orthopaedic Research

Self Cite

View full text Add to dashboard Cite

This study presents a novel biodegradable alginate delivery system for antibiotics and bone cells to treat infected bone defects. About 2 Â 10 7 New Zealand rabbit mesenchymal stem cells (MSCs) and 5 mL vancomycin solution (50 mg/mL) were added to 5 mL of 2.5% (w/v) sodium alginate solution to form biodegradable antibiotic and MSCs alginate beads 3 mm in diameter. The alginate beads were then cultured in an osteogenic medium for 14 days. The profiles of antibiotics released from the alginate beads were evaluated using the method of high performance liquid chromatography (HPLC). The expression of osteogenic genes, including Cbfa1 and osteopontin, in the alginate beads was determined by reverse transcription-polymerase chain reaction (RT-PCR) analysis. The alkaline phosphatase activity, calcium level, and mineral deposition of the cultured cells within the alginate beads were assessed. Analytical results demonstrated that the concentrations of vancomycin eluted from the alginate beads were, for 14 days, well above the minimal inhibitory concentration of Staphylococcus aureus. Osteopontin and mRNA of Cbfa1 were detected and increased alkaline phosphatase activity and calcium levels were noted, as was a substantial mineral deposition in cultured MSCs. The PKH 26-labeled MSCs and vancomycin alginate beads were implanted in rabbit bony cavities for in vivo analysis. Implanted PKH 26-labeled MSCs were identified in the newly formed bony trabeculae in all specimens at 2 and 4 months after implantation and there was abundant mineral deposition. The results of in vitro study demonstrated sustained elution of vancomycin from the alginate carrier for 14 days and good osteogenic differentiation of cultured MSCs in the alginate carrier matrix. The results of in vivo study demonstrated the implanted MSCs participating in new bone formation. Based on experimental evidence, development of a biodegradable alginate carrier system for antibiotics and bone cells is possible, providing a potential treatment procedure for infected bone defects. ß

show abstract

“…In general, this sustained release rate was due to the novel 3D hierarchically nanostructures with mesoporous structure. In clinical applications, the breakpoint sensitivity of vancomycin for Staphylococcus aureus was 5 µg/mL, respectively[21]. The vancomycin concentrations released from HAp microspheres during the research period was around 76-155µg/mL…”

mentioning

confidence: 97%